Mycobacterium paratuberculosis as a cause of Crohn’s disease

McNees, Adrienne L.; Markesich, Diane C.; Zayyani, Najah R; Graham, David Y.

doi:10.1586/17474124.2015.1093931

Cited by 122 publications

(96 citation statements)

References 130 publications

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“…; McNees et al . ). The economic damage of paratuberculosis is a serious problem not only in cow milk production but also in sheep and goat holdings (Ayele et al .…”

Section: Introductionmentioning

confidence: 97%

Development and validation of a loop‐mediated isothermal amplification assay—a rapid and sensitive detection tool forMycobacterium aviumsubsp.paratuberculosisin small ruminants

et al. 2019

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Aims The aim of this study was to design an assay for the identification of Mycobacterium avium subsp. paratuberculosis (MAP) to be used in faeces and milk samples of small ruminants with a loop‐mediated isothermal amplification (LAMP) system, as a time‐saving and user‐friendly method in contrast to real‐time PCR. Methods and Results For the detection of MAP in milk and faeces of small ruminants, we developed a set of primers, specific for the target gene ISMap02. The analytical sensitivity of LAMP, when targeting ISMap02, showed a DNA detection limit of 10 fg μl−1. After performing spiking experiments with two MAP reference strains, DSM 44133 and ATCC 19698T, the limit of detection, using the LAMP protocol described herein were 3·8 MAP CFU per ml milk and 12·5 MAP CFU per gram faeces. All LAMP results during the establishment of the assay were compared to those of the real‐time PCR results. An internal amplification control was incorporated into the assay to exclude false‐negative results produced and had no significant negative impact on the analytical sensitivity. Validation of the assay was confirmed by testing field samples of faeces and revising the results with real‐time PCR. Conclusion Our study conducted the first MAP detection system with a LAMP targeting ISMap02. Due to the positive results we encourage the use of LAMP in combination with ISMap02, when detecting MAP in faeces samples, as an alternative to targeting other genes as f57 or IS900. Further research on MAP detection in different matrices like raw milk, tissue or sperm with this system is recommended. Significance and Impact of the Study This study provides new achievements in MAP diagnostic. Especially small ruminants do not show signs of diarrhoea until the terminal stage of the illness. The greatest task in fighting MAP is to rule out animals, which shed MAP with faeces and milk before showing symptoms of Johne's disease. Worldwide there is a need to eradicate animals, which are low MAP shedders to stop the illness spreading in animal holdings. MAP detection with LAMP is time saving, easy to use, does not need expensive equipment, as, for example, PCR kits and can be used without access to laboratories. The target gene ISMap02 was shown to be a specific insertion element for MAP and is a reliable aim in future MAP detection studies.

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“…; McNees et al . ). The economic damage of paratuberculosis is a serious problem not only in cow milk production but also in sheep and goat holdings (Ayele et al .…”

Section: Introductionmentioning

confidence: 97%

Development and validation of a loop‐mediated isothermal amplification assay—a rapid and sensitive detection tool forMycobacterium aviumsubsp.paratuberculosisin small ruminants

et al. 2019

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“…in human beings (McFadden et al, 1992;Bull et al, 2003;Abubakar et al, 2007;Scanu et al, 2007;Cirone et al, 2007;Naser et al, 2014;Banche et al, 2015;McNees et al, 2015). Live bacilli (MAP) have been recovered from milk and milk products made from laboratory scale and commercial size pasteurization facilities for liquid milk in Czech Republic, UK, USA and India (Grant et al, 1996;Shankar et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

BIO-INCIDENCE OF Mycobacterium avium SUBSPECIES paratuberculosis IN THE PASTEURIZED LIQUID MILK, FLAVOURED MILK AND MILK POWDER COMMERCIALLY SOLD BY LEADING MARKET BRANDS IN INDIA

Singh¹,

Singh²,

Gupt³

et al. 2018

JEBAS

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“…Comparatively, it has been documented that there is an overlap in the genes responsible for the immunemediated mechanism of IBD and the immune response to mycobacteria infection, including Mycobacterium tuberculosis and Mycobacterium leprae. 1,4 As such, comparison of the morphologic features of IBD and intestinal TB may be necessary in countries where there is an increasing incidence of one and a consistently high incidence of the other.…”

Section: Introductionmentioning

confidence: 99%

The Morphologic Profile of Inflammatory Bowel Disease and the Diagnostic Problem of Crohn’s Disease versus TB Colitis– A Case Series

Tilbe

Reyes

et al. 2017

PJP

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The aim of this study is to describe the morphologic profile of the biopsy and resection specimen that were diagnosed with Crohn's disease and ulcerative colitis in the University of the East Ramon Magsaysay Memorial Medical Center (UERMMMC) from [2008][2009][2010][2011][2012][2013][2014][2015][2016]. Features that classify the specimen as Inflammatory Bowel Disease -Indeterminate Type are also presented. Considerations for the definitive IBD classification after an initial indeterminate diagnosis by morphology are also briefly discussed. Biopsy and resection specimen that were diagnosed with Crohn's disease, cannot exclude TB Colitis, are also presented; and the subsequent steps for a definitive classification are also discussed. All the patients included underwent an endoscopic biopsy, and are categorized by histopathologic diagnoses, age, sex, and GIT segment involved in the endoscopic procedure. Patients that underwent subsequent resection due to the disease condition are also identified.Comparison of the histologic findings observed in the patients, with the microscopic basis for the diagnosis recommended by the European consensus on the histopathology of inflammatory bowel disease (2013), and with the histologic features described by Patil et al., (2015) for the inflammatory disorders of the large intestine, is done. The histologic features described by Lamps (2015) for the gastrointestinal TB is used in the evaluation of the findings in the patients diagnosed with Crohn's disease, cannot exclude TB Colitis.There are 5 Crohn's disease patients, accounting for 0.8% of all patients with lower GIT inflammatory conditions, and 10 ulcerative colitis patients, accounting for 1.6% of all patients with lower GIT inflammatory conditions. Seven patients, which comprise 1.1% of all patients with lower GIT inflammatory conditions, have the diagnosis of indeterminate colitis. The histologic features of 6 out of 7 patients that had the initial diagnosis of indeterminate colitis presented with morphologic features that favored an ulcerative colitis, but with Crohn's disease features. In comparison, one patient who had an initial diagnosis of indeterminate colitis presented with morphologic features that favored Crohn's disease but with ulcerative colitis features. In these patients, correlation with chronology of symptoms and associated ancillary procedures that can classify the patients as CD or UC are recommended to the gastroenterologist attending such patients so that a more definitive classification can be done.Four patients, accounting for 0.6% of all lower GIT inflammatory conditions, were initially diagnosed as Crohn's disease, cannot exclude TB Colitis. This is in contrast with 34 patients who were diagnosed with Chronic Granulomatous Inflammation, Tuberculosis which accounted for 5.7% of all patients that were diagnosed with inflammatory conditions of the lower GIT. The remaining 536 patients were composed of acute self-limited/ infectious colitis, ischemic colitis, eosinophilic colitis, inflammatory ...

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Mycobacterium paratuberculosis as a cause of Crohn’s disease

Cited by 122 publications

References 130 publications

Development and validation of a loop‐mediated isothermal amplification assay—a rapid and sensitive detection tool forMycobacterium aviumsubsp.paratuberculosisin small ruminants

Development and validation of a loop‐mediated isothermal amplification assay—a rapid and sensitive detection tool forMycobacterium aviumsubsp.paratuberculosisin small ruminants

BIO-INCIDENCE OF Mycobacterium avium SUBSPECIES paratuberculosis IN THE PASTEURIZED LIQUID MILK, FLAVOURED MILK AND MILK POWDER COMMERCIALLY SOLD BY LEADING MARKET BRANDS IN INDIA

The Morphologic Profile of Inflammatory Bowel Disease and the Diagnostic Problem of Crohn’s Disease versus TB Colitis– A Case Series

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