“Mycobacterium massilipolynesiensis” sp. nov., a rapidly-growing mycobacterium of medical interest related to Mycobacterium phlei

Phelippeau, Michael; Asmar, Shady; Osman, Djaltou Aboubaker; Sassi, Mohamed; Robert, Cédric; Michelle, Caroline; Musso, Didier; Drancourt, Michel

doi:10.1038/srep40443

Cited by 6 publications

(3 citation statements)

References 41 publications

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“…org) using the model organism M. smegmatis mc 2 155 [31][32][33]. Despite the enormous number of phages identified, there is a lack of studies on their occurrence in other Mycolicibacterium species [99][100][101]. Using PHASTER, a total of 566 prophages were predicted in 229/242 Mycolicibacterium genomes (~94 %) (File S4).…”

Section: Identification and Characterization Of Mycolicibacterium Promentioning

confidence: 99%

Comprehensive in silico survey of the Mycolicibacterium mobilome reveals an as yet underexplored diversity

Morgado

Moreno-Vicente

2021

Microbial Genomics

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The mobilome plays a crucial role in bacterial adaptation and is therefore a starting point to understand and establish the gene flow occurring in the process of bacterial evolution. This is even more so if we consider that the mobilome of environmental bacteria can be the reservoir of genes that may later appear in the clinic. Recently, new genera have been proposed in the family Mycobacteriaceae , including the genus Mycolicibacterium , which encompasses dozens of species of agricultural, biotechnological, clinical and ecological importance, being ubiquitous in several environments. The current scenario in the Mycobacteriaceae mobilome has some bias because most of the characterized mycobacteriophages were isolated using a single host strain, and the few plasmids reported mainly relate to the genus Mycobacterium . To fill in the gaps in these issues, we performed a systematic in silico study of these mobile elements based on 242 available genomes of the genus Mycolicibacterium . The analyses identified 156 putative plasmids (19 conjugative, 45 mobilizable and 92 non-mobilizable) and 566 prophages in 86 and 229 genomes, respectively. Moreover, a contig was characterized by resembling an actinomycete integrative and conjugative element (AICE). Within this diversity of mobile genetic elements, there is a pool of genes associated with several canonical functions, in addition to adaptive traits, such as virulence and resistance to antibiotics and metals (mercury and arsenic). The type-VII secretion system was a common feature in the predicted plasmids, being associated with genes encoding virulent proteins (EsxA, EsxB, PE and PPE). In addition to the characterization of plasmids and prophages of the family Mycobacteriaceae , this study showed an abundance of these genetic elements in a dozen species of the genus Mycolicibacterium .

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Section: Identification and Characterization Of Mycolicibacterium Promentioning

confidence: 99%

Comprehensive in silico survey of the Mycolicibacterium mobilome reveals an as yet underexplored diversity

Morgado

Moreno-Vicente

2021

Microbial Genomics

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“…Because of the potential role of this new species mycobacterium in the clinical situation of the patient could not be derived from the sole isolate here reported, antibiotic susceptibility profile here reported was performed to contribute to the phenotypic characterization of the isolate and not to guide in anyway antibiotic treatment. Interestingly, this potential new species has been found in French Polynesia where a previous investigation using the same molecular tools, yielded another new Mycobacterium species, Mycobacterium massilipolynesiensis , in the Mycobacterium simiae complex 17 . In French Polynesia also, genotyping techniques allowed the discovery of another nontuberculous Mycobacterium species and new Mycobacterium tuberculosis lineages 18 .…”

Section: Discussionmentioning

confidence: 84%

“Mycobacterium mephinesia”, a Mycobacterium terrae complex species of clinical interest isolated in French Polynesia

Saad¹,

Phelippeau²,

Khoder³

et al. 2019

Sci Rep

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A 59-year-old tobacco smoker male with chronic bronchitis living in Taravao, French Polynesia, Pacific, presented with a two-year growing nodule in the middle lobe of the right lung. A guided bronchoalveolar lavage inoculated onto Löwenstein-Jensen medium yielded colonies of a rapidly-growing non-chromogenic mycobacterium designed as isolate P7213. The isolate could not be identified using routine matrix-assisted laser desorption ionization-time of flight-mass spectrometry and phenotypic and probe-hybridization techniques and yielded 100% and 97% sequence similarity with the respective 16S rRNA and rpoB gene sequences of Mycobacterium virginiense in the Mycobacterium terrae complex. Electron microscopy showed a 1.15 µm long and 0.38 µm large bacillus which was in vitro susceptible to rifampicin, rifabutin, ethambutol, isoniazid, doxycycline and kanamycin. Its 4,511,948-bp draft genome exhibited a 67.6% G + C content with 4,153 coding-protein genes and 87 predicted RNA genes. Genome sequence-derived DNA-DNA hybridization, OrthoANI and pangenome analysis confirmed isolate P7213 was representative of a new species in the M . terrae complex. We named this species “ Mycobacterium mephinesia ”.

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“…Niacin production was detected using BBL™ Taxo™ TB Niacin test strips (Becton Dickinson) as described by the manufacturer. Tween 80 hydrolysis test was performed as previously described 21 .…”

Section: Methodsmentioning

confidence: 99%

Pulmonary Isolation of Multidrug resistant “Mycobacterium simulans” and Mycobacterium tuberculosis from a patient in the Horn of Africa

Bouzid

Osman

Baptiste

et al. 2018

Sci Rep

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In low-income countries of the Horn of Africa, pulmonary infections are usually considered as tuberculosis, which diagnosis relies on clinical data and positive microscopic observation. This strategy allows non-tuberculous mycobacteria to escape detection, facilitating their emergence in populations. A non-tuberculous mycobacterium strain FB-527 was unexpectedly cultured from the sputum of a Djiboutian patient otherwise diagnosed with multi-drug resistant (MDR) tuberculosis. The sequencing of the rpoB and 16S rRNA genes showed that the isolate was identical to strain FI-09026 previously named “Mycobacterium simulans” and reported only once from a Somali patient. Strain FB-527 mimicked Mycobacterium tuberculosis colonies and enzymatic profile using API ZYM strip and was in vitro resistant to rifampicin and isoniazid. Isolation of two MDR mycobacteria complicated the diagnosis and therapeutic management of the patient. We here report on the complete description of strain FB-527 and strain FI-09026 including genome sequencing, finalizing the description of the proposed new species “Mycobacterium simulans”.

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“Mycobacterium massilipolynesiensis” sp. nov., a rapidly-growing mycobacterium of medical interest related to Mycobacterium phlei

Cited by 6 publications

References 41 publications

Comprehensive in silico survey of the Mycolicibacterium mobilome reveals an as yet underexplored diversity

Comprehensive in silico survey of the Mycolicibacterium mobilome reveals an as yet underexplored diversity

“Mycobacterium mephinesia”, a Mycobacterium terrae complex species of clinical interest isolated in French Polynesia

Pulmonary Isolation of Multidrug resistant “Mycobacterium simulans” and Mycobacterium tuberculosis from a patient in the Horn of Africa

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