Mycobacterium leprae genotype amplified from an archaeological case of lepromatous leprosy in Central Asia

Taylor, G. Michael; Blau, Soren; Mays, Simon; Monot, Marc; Lee, Oona Y.-C.; Minnikin, David E.; Besra, Gurdyal S.; Cole, Stewart T.; Rutland, Paul

doi:10.1016/j.jas.2009.06.026

Cited by 45 publications

(40 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Techniques [11] 2012-2017 SNP sub-genotyping and WGS Mycocerosic acid lipid biomarkers identified SNP sub-genotyping elucidates geographical differences between M. leprae from different regions [14,[38][39][40] 2. Methods…”

Section: Yearmentioning

confidence: 99%

“…A further development has been the use of organic chemical techniques to detect and identify specific mycobacterial cell wall lipids in ancient cases of tuberculosis [9,10] and leprosy [11][12][13]. Indeed, these specific cell wall lipids are more stable than aDNA and have confirmed suspected cases of ancient leprosy in the absence of M. leprae aDNA [14].…”

Section: Introductionmentioning

confidence: 99%

“…The growth of this field of paleomicrobiology is summarized in Table 1 for ancient tuberculosis [4,5,9,10,[22][23][24][25][26][27][28][29][30][31][32][33] and in Table 2 for ancient leprosy [6,11,14,18,[34][35][36][37][38][39][40], highlighting the changes in methodology over time. Detailed information for a range of studies on ancient tuberculosis is provided in Table S1 [4,5,9,10,[22][23][24][25][26][27][28][29][30][31][32][33]36,.…”

Section: Introductionmentioning

confidence: 99%

“…Detailed information for a range of studies on ancient tuberculosis is provided in Table S1 [4,5,9,10,[22][23][24][25][26][27][28][29][30][31][32][33]36,. Similarly, studies on ancient leprosy are tabulated in Table S2 [6,[11][12][13][14]18,20,[34][35][36][37][38][39][40]53,[68][69][70][71][72][73][74][75][76].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Positive Diagnosis of Ancient Leprosy and Tuberculosis Using Ancient DNA and Lipid Biomarkers

et al. 2017

View full text Add to dashboard Cite

Diagnosis of leprosy and tuberculosis in archaeological material is most informative when based upon entire genomes. Ancient DNA (aDNA) is often degraded but amplification of specific fragments also provides reliable diagnoses. Cell wall lipid biomarkers can distinguish ancient leprosy from tuberculosis and DNA extraction residues can be utilized. The diagnostic power of combined aDNA and lipid biomarkers is illustrated by key cases of ancient leprosy and/or tuberculosis. Human tuberculosis was demonstrated in a woman and child from Atlit-Yam (~9 ka) in the Eastern Mediterranean and in the 600 BCE Egyptian "Granville" mummy. Both aDNA and lipids confirmed Pleistocene tuberculosis in a~17 ka bison from Natural Trap Cave, Wyoming. Leprosy is exemplified by cases from Winchester (10th-12th centuries CE) and Great Chesterford (5th-6th centuries CE). A mixed infection from Kiskundorozsma, Hungary (7th century CE) allowed lipid biomarkers to assess the relative load of leprosy and tuberculosis. Essential protocols for aDNA amplification and analysis of mycolic, mycolipenic, mycocerosic acid, and phthiocerol lipid biomarkers are summarized. Diagnoses of ancient mycobacterial disease can be extended beyond the reach of whole genomics by combinations of aDNA amplification and lipid biomarkers, with sole use of the latter having the potential to recognize even older cases.

show abstract

Section: Yearmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Positive Diagnosis of Ancient Leprosy and Tuberculosis Using Ancient DNA and Lipid Biomarkers

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Leprosy is an ancient disease which has been spread among human populations by migrations, military expansion and colonization, as well as along trade routes [1,2]. According to the history, the disease of leprosy entering Indonesia was allegedly carried away from China and India to the island of Java.…”

Section: Introductionmentioning

confidence: 99%

Variation of TTC Repeat Pattern In The Dna of Mycobacterium Leprae Isolates Obtained from Archeological Bones and Leprosy Patients From East Nusa Tenggara

Adriaty¹,

Wahyuni²,

Iswahyudi³

et al. 2012

JTLS

View full text Add to dashboard Cite

The existence of leprosy or kusta or Morbus Hansen or Hansen's disease has been known for years, including in Indonesia. Starting from the discovery of Mycobacterium leprae isolates from ancient bone (about 1.000 years B.C), the archaeological excavations results in East Nusa Tenggara, interesting questions arise about how the development of leprosy in eastern Indonesia is. Biology molecular study would become a powerful tool to investigate the presence of leprosy bacillary whether there are similarities between the genomes of M. leprae isolates in the primeval and the present. PCR examinations were performed on mandibular bone fragments from ancient human who lived 1000 years B.C. discovered in archaeological surveys on the island of Lembata and three leprosy patients from East Nusa Tenggara. The DNA extraction was performed using a kit from Qiagen products and its TTC repeating pattern was seen with the method of direct sequencing. It turned out that the TTC profile obtained from samples of archaeological was as many as 13 copies, while the repetition of TTC in three samples of leprosy patients were 15, 17 and 26 copies. The different number of TTC repetition shows the different isolates of M. leprae between in the ancient times and the present. Further studies are needed to verify the differences in the genome that occur, for example from the study of SNPs (single nucleotide polymorphisms).

show abstract

Human Remains

Roberts

2013

Archaeology in Practice

View full text Add to dashboard Cite

Mycobacterium leprae genotype amplified from an archaeological case of lepromatous leprosy in Central Asia

Cited by 45 publications

References 19 publications

Positive Diagnosis of Ancient Leprosy and Tuberculosis Using Ancient DNA and Lipid Biomarkers

Positive Diagnosis of Ancient Leprosy and Tuberculosis Using Ancient DNA and Lipid Biomarkers

Variation of TTC Repeat Pattern In The Dna of Mycobacterium Leprae Isolates Obtained from Archeological Bones and Leprosy Patients From East Nusa Tenggara

Human Remains

Contact Info

Product

Resources

About