Mycobacterium iranicum sp. nov., a rapidly growing scotochromogenic species isolated from clinical specimens on three different continents

Shojaei, Hasan; Daley, Charles L.; Gitti, Zoe; Hashemi, Abodolrazagh; Heidarieh, Parvin; Moore, Edward R. B.; Naser, Abass Daei; Russo, Cristina; Ingen, Jakko van; Tortoli, Enrico

doi:10.1099/ijs.0.043562-0

Cited by 48 publications

(58 citation statements)

References 21 publications

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“…The strain obtained from this patient's sputum was identified as M. iranicum on the basis of genotypic characteristics. The first third of the 16S rRNA gene was identical to that of the type strain and all the other strains of M. iranicum reported to date (12), while this patient's isolate differed from the type strain by two hsp65 gene nucleotides.…”

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confidence: 59%

“…iranicum isolates have historically originated from patients residing in Mediterranean countries (12). Although several reports suggest a point source exposure for patients with newly diagnosed NTM infections (5,14), the source of infection in this patient was not determined.…”

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confidence: 95%

“…Genotypically, major conserved genetic regions allow differentiation of multiple sequevars. Of the eight reported M. iranicum strains, five were isolated from sputum of patients with various pulmonary disorders; clinical significance, according to the criteria of the American Thoracic Society (ATS), was attributed to at least one isolate from Iran (12,13). The strain obtained from this patient's sputum was identified as M. iranicum on the basis of genotypic characteristics.…”

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confidence: 99%

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Isolation of a Novel Strain of Mycobacterium iranicum from a Woman in the United States

et al. 2013

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Isolation of a Novel Strain of Mycobacterium iranicum from a Woman in the United States

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“…It is a rapidly growing scotochromogenic species with a yelloworange pigment. Based on a few housekeeping genes, a phylogenetic analysis showed that M. iranicum is closely related to another rapid grower, M. gilvum (5). Ten cases of M. iranicum infection have been reported, including pneumonia, meningitis, and soft tissue infection (5-7).…”

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confidence: 99%

Successful Treatment of Peritoneal Dialysis-related Peritonitis due to <i>Mycobacterium iranicum</i>

et al. 2016

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A 68-year-old man on peritoneal dialysis (PD) was hospitalized with the clinical picture of peritonitis. The patient was diagnosed with peritonitis caused by nontuberculous mycobacteria (NTM) according to positive Ziehl-Neelsen staining and negative Mycobacterium tuberculosis polymerase chain reaction results. Oral levofloxacin and clarithromycin, and later intraperitoneal imipenem were started. According to the anti-NTM susceptibility test results, oral minocycline was administered. The patient was treated for 6 months. He recovered without PD catheter removal; thus, PD was successfully continued. A genetic analysis identified the isolate as Mycobacterium iranicum. This is the first report of PD-related peritonitis caused by M. iranicum.

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“…Eleven isolates had matching results in both 16S rRNA gene and rpoB gene sequencing and were identified as (14). Two isolates showed more than 98.3% similarity with established species by rpoB gene sequencing but showed one base pair mismatch with 16S rRNA gene sequencing: M. phlei (n ϭ 1) and M. novocastrense (n ϭ 1).…”

Section: Screening Of 455 Ntm Isolates Using the Reverse Line Blot Asmentioning

confidence: 99%

Utility of rpoB Gene Sequencing for Identification of Nontuberculous Mycobacteria in the Netherlands

2014

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bIn the Netherlands, clinical isolation of nontuberculous mycobacteria (NTM) has increased over the past decade. Proper identification of isolates is important, as NTM species differ strongly in clinical relevance. Most of the currently applied identification methods cannot distinguish between all different Mycobacterium species and complexes within species. rpoB gene sequencing exhibits a promising level of discrimination among rapidly and slowly growing mycobacteria, including the Mycobacterium avium complex. In this study, we prospectively compared rpoB gene sequencing with our routine algorithm of reverse line blot identification combined with partial 16S rRNA gene sequencing of 455 NTM isolates. rpoB gene sequencing identified 403 isolates to species level as 45 different known species and identified 44 isolates to complex level, and eight isolates remained unidentifiable to species level. In contrast, our reference reverse line blot assay with adjunctive 16S rRNA gene sequencing identified 390 isolates to species level (30 distinct species) and identified 56 isolates to complex level, and nine isolates remained unidentified. The higher discriminatory power of rpoB gene sequencing results largely from the distinction of separate species within complexes and subspecies. Also, Mycobacterium gordonae, Mycobacterium kansasii, and Mycobacterium interjectum were separated into multiple groupings with relatively low sequence similarity (98 to 94%), suggesting that these are complexes of closely related species. We conclude that rpoB gene sequencing is a more discriminative identification technique than the combination of reverse line blot and 16S rRNA gene sequencing and could introduce a major improvement in clinical care of NTM disease and the research on the epidemiology and clinical relevance of NTM.

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Mycobacterium iranicum sp. nov., a rapidly growing scotochromogenic species isolated from clinical specimens on three different continents

Cited by 48 publications

References 21 publications

Isolation of a Novel Strain of Mycobacterium iranicum from a Woman in the United States

Isolation of a Novel Strain of Mycobacterium iranicum from a Woman in the United States

Successful Treatment of Peritoneal Dialysis-related Peritonitis due to <i>Mycobacterium iranicum</i>

Utility of rpoB Gene Sequencing for Identification of Nontuberculous Mycobacteria in the Netherlands

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