Mycobacterium genavense Infection in a Patient with AIDS Who Was Successfully Treated with Clarithromycin

Matsiota-Bernard, P.; Thierry, Dominique; Truchis, Pierre de; Saillour, M.; Paraire, F.; Guesdon, J L; Nauciel, C

doi:10.1093/clinids/20.6.1565-a

Cited by 16 publications

(18 citation statements)

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“…Limited information suggests drugs including clarithromycin, rifampicin and clofazimine, have efficacy against M genavense, as witnessed by human patients, an affected dog and the ferrets of the present report, which improved substantially or were cured using multidrug regimens. [29][30][31] Studies using a mouse model have shown clarithromycin and rifambutin to be most effective, whereas ciprofloxacin was ineffective. 32 Based on limited published information, clarithromycin would appear to be the most effective agent.…”

Section: Discussionmentioning

confidence: 99%

Mycobacterium genavenseinfection in two aged ferrets with conjunctival lesions

Lucas

Furber²,

James

et al. 2000

Aust Veterinary J

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Mycobacterium genavense infection was diagnosed in two adult ferrets. Disseminated mycobacteriosis was diagnosed in a castrated 5-year-old sable ferret with generalised peripheral lymph node enlargement and a proliferative lesion of the conjunctiva of the nictitating membrane. The diagnosis was based on characteristic cytology and sequence analysis of the 16S rRNA gene amplified using the polymerase chain reaction from fresh biopsy material. Therapy with rifampicin, clofazimine and clarithromycin probably cured the infection. An entire 4-year-old female ferret with conjunctival swelling, serous ocular discharge and swelling of the subcutaneous tissues of the nasal bridge was diagnosed as having M genavense infection on the basis of typical cytology, histopathology and sequence analysis of 16S rRNA amplicons from formalin-fixed paraffin-embedded tissue. This patient was treated successfully using rifampicin. Both ferrets subsequently died as a result of other disease conditions, 10 and 4 months following initiation of therapy, respectively. This is the first report documenting M genavense as a cause of disseminated mycobacterial disease in ferrets. Conjunctival involvement may be a feature of disseminated mycobacteriosis in the ferret. The possibility that these infections were the consequence of a ferret retrovirus infection should be considered further.

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Section: Discussionmentioning

confidence: 99%

Mycobacterium genavenseinfection in two aged ferrets with conjunctival lesions

Lucas

Furber²,

James

et al. 2000

Aust Veterinary J

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“…Combined amikacin, clofazimine, clarithromycin, rifampin, ethambutol, and ciprofloxacin were successful in treating another child with AIDS and disseminated M. genavense infection (371). A patient with AIDS infected with M. genavense was successfully treated with clarithromycin (340).…”

Section: Chemotherapy Of M Genavense Infectionmentioning

confidence: 99%

Epidemiology of infection by nontuberculous mycobacteria

1996

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“…M. genavense strain B.C. was isolated from an AIDS patient's blood as already described [23]. Suspensions of M. genavense strains IMT95–610, IMT95–615, IMT95–1160, IMT96–1799, IMT96–1802, IMT97–76 were kindly provided by Prof. Françoise Portaels (Institute of Tropical Medicine, Antwerp, Belgium), and M. genavense strains (1958, 4182, 2289, 2281, 4096, 1288) were obtained from Dr. M.B.…”

Section: Methodsmentioning

confidence: 99%

Isolation of a specific DNA fragment and development of a PCR-based method for the detection ofMycobacterium genavense

Chevrier

Oprişan

Maresca

et al. 1999

FEMS Immunology &Amp; Medical Microbiology

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The rise of Mycobacterium genavense infections is making identification ever more important for diagnosis and treatment. Moreover, isolation and identification of M. genavense are made difficult by the lack of growth on solid media and by its low generation rate in BACTEC liquid media. Thus, amplification by PCR or similar techniques represents the only possibility of detecting and identifying M. genavense from tissue samples. In order to set up a simple and species-specific method based on the use of PCR and non-radioactive hybridization technique, we decided to search for and clone a specific DNA fragment of this bacterial species. In the present study, a 1734-bp fragment was isolated. This fragment was found to be highly specific for M. genavense strains. A species-specific pair of primers (MG22 and MG23) and two oligonucleotide probes (MG18 and MG19) were selected. They were successfully used to amplify and detect a 155-bp DNA fragment from the 13 available strains of M. genavense which were isolated from clinical specimens or from birds. Conversely, the primers and probes did not hybridize with DNA from any of the 20 other mycobacterial species tested. It is worth noting that the chosen primers and probes did not hybridize with DNA of M. simiae, although it is closely related to M. genavense. The present PCR technique uses species-specific primers for M. genavense. Followed by a non-radioactive hybridization technique on microplates it is able to distinguish M. genavense from other mycobacteria in one step, without sequencing or restriction analysis. On the basis of the Southern blot hybridization, PCR and sandwich hybridization results, we concluded that the isolated 1.7-kb sequence was specific for the M. genavense chromosome. The method developed here for M. genavense identification uses a simple methodology and commonly available reagents. Furthermore it can be easily automated.

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Mycobacterium genavense Infection in a Patient with AIDS Who Was Successfully Treated with Clarithromycin

Cited by 16 publications

References 0 publications

Mycobacterium genavenseinfection in two aged ferrets with conjunctival lesions

Mycobacterium genavenseinfection in two aged ferrets with conjunctival lesions

Epidemiology of infection by nontuberculous mycobacteria

Isolation of a specific DNA fragment and development of a PCR-based method for the detection ofMycobacterium genavense

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