Mycobacterium Chimaera: A Rare Presentation

Miskoff, Jeffrey A; Chaudhri, Moiuz

doi:10.7759/cureus.2750

Cited by 7 publications

(8 citation statements)

References 8 publications

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“…Because Mycobacterium sp. are commonly found in soil, dust, and water [36], the bacterial contamination in the DNA may come from plant surfaces, but some studies have also shown that they may be endophytes in plants [37][38][39]. Some evidence suggests that endophytes may have a positive effect on plants [40,41], but Mycobacterium sp.…”

Section: Discussionmentioning

confidence: 99%

Genome Survey Sequencing of Betula platyphylla

Wang

Chen

Liu

et al. 2019

Forests

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Research Highlights: A rigorous genome survey helped us to estimate the genomic characteristics, remove the DNA contamination, and determine the sequencing scheme of Betula platyphylla. Background and Objectives: B. platyphylla is a common tree species in northern China that has high economic and medicinal value. However, there is a lack of complete genomic information for this species, which severely constrains the progress of relevant research. The objective of this study was to survey the genome of B. platyphylla and determine the large-scale sequencing scheme of this species. Materials and Methods: Next-generation sequencing was used to survey the genome. The genome size, heterozygosity rate, and repetitive sequences were estimated by k-mer analysis. After preliminary genome assembly, sequence contamination was identified and filtered by sequence alignment. Finally, we obtained sterilized plantlets of B. platyphylla by plant tissue culture, which can be used for third-generation sequencing. Results: We estimated the genome size to be 432.9 Mb and the heterozygosity rate to be 1.22%, with repetitive sequences accounting for 62.2%. Bacterial contamination was observed in the leaves taken from the field, and most of the contaminants may be from the genus Mycobacterium. A total of 249,784 simple sequence repeat (SSR) loci were also identified in the B. platyphylla genome. Among the SSRs, only 11,326 can be used as candidates to distinguish the three Betula species. Conclusions: The B. platyphylla genome is complex and highly heterozygous and repetitive. Higher-depth third-generation sequencing may yield better assembly results. Sterilized plantlets can be used for sequencing to avoid contamination.

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Section: Discussionmentioning

confidence: 99%

Genome Survey Sequencing of Betula platyphylla

Wang

Chen

Liu

et al. 2019

Forests

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“…First identified in 2004, M. chimaera was responsible for a global outbreak linked to contaminated heater-cooler devices used in cardiac bypass surgery [8,9]. With a 50-60% mortality rate and prolonged treatment regimen of 12-18 months with an antibiotic cocktail of macrolides, ethambutol and rifamycins, new, better tolerated and more rapidly effective regimens are required [10].…”

Section: Introductionmentioning

confidence: 99%

In vitro activity of SPR719 against Mycobacterium ulcerans, Mycobacterium marinum and Mycobacterium chimaera

et al. 2021

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Nontuberculosis mycobacterial (NTM) infections are increasing in prevalence across the world. In many cases, treatment options for these infections are limited. However, there has been progress in recent years in the development of new antimycobacterial drugs. Here, we investigate the in vitro activity of SPR719, a novel aminobenzimidazole antibiotic and the active form of the clinical-stage compound, SPR720, against several isolates of Mycobacterium ulcerans, Mycobacterium marinum and Mycobacterium chimaera. We show that SPR719 is active against these NTM species with a MIC range of 0.125–4 μg/ml and that this compares favorably with the commonly utilized antimycobacterial antibiotics, rifampicin and clarithromycin. Our findings suggest that SPR720 should be further evaluated for the treatment of NTM infections.

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“…Bills et al in 2009 describes a patient with a history of COPD and smoking [4]. A case report by Miskoff et al in 2018 described one patient presenting with underlying COPD [5]. The reason so few cases of patients with the underlying pulmonary disease have been reported may be due to its recent classification.…”

Section: Discussionmentioning

confidence: 99%

“…Respiratory disease outbreaks are linked to the formation of biofilms in heated stagnant water, which is a favorable environment for the pathogen [2,3]. Other NTB pathogens are associated with patients with underlying pulmonary disease, but this has only been seen in case reports regarding Mycobacterium chimaera specifically [1,4,5].…”

Section: Introductionmentioning

confidence: 99%