Mycobacterium caprae transmission to free-living grey wolves (Canis lupus) in the Bieszczady Mountains in Southern Poland

Orłowska, Blanka; Augustynowicz–Kopeć, Ewa; Krajewska, Monika; Zabost, Anna; Welz, Mirosław; Kaczor, Stanisław; Anusz, Krzysztof

doi:10.1007/s10344-017-1079-4

Cited by 23 publications

(27 citation statements)

References 15 publications

(12 reference statements)

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“…It is also possible that foxes, when they feed on carcasses, are not left with much, because the infected tissues have been eaten by the larger predators. It should be noted that M.caprae have been isolated from wolves in the Bieszczady [5].…”

Section: Discussionmentioning

confidence: 99%

Epidemiological characterization of Mycobacterium caprae strains isolated from wildlife in the Bieszczady Mountains, on the border of Southeast Poland

et al. 2020

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Background The majority of animal tuberculosis (TB) cases reported in wildlife in Poland over the past 20 years have concerned the European bison inhabiting the Bieszczady Mountains in Southeast Poland: an area running along the border of Southeast Poland. As no TB cases have been reported in domestic animals in this region since 2005, any occurrence of TB in the free-living animals inhabiting this area might pose a real threat to local livestock and result in the loss of disease-free status. The aim of the study was to describe the occurrence of tuberculosis in the wildlife of the Bieszczady Mountains and determine the microbiological and molecular characteristics of any cultured strains. Lymph node samples were collected for analysis from 274 free-living animals, including European bison, red foxes, badgers, red deer, wild boar and roe deer between 2011 and 2017. Löwenstein–Jensen and Stonebrink media were used for culture. Molecular identification of strains was performed based on hsp65 sequence analysis, the GenoType®MTBC (Hain Lifescience, Germany) test, spoligotyping and MIRU-VNTR analysis. Results Mycobacterium caprae was isolated from the lymph nodes of 21 out of 55 wild boar (38.2%; CI 95%: 26.5%, 51.4%) and one roe deer. Since 2014, no new TB cases have been reported in the Bieszczady European bison population. Conclusions The identification of TB in wild boar in the Bieszczady is an alarming phenomenon, which requires further investigation. The Bieszczady mountains are a precious, unique area, home to many protected species. However, it is also the only area in Poland where TB cases have been reported in free-living animals. The occurrence of TB in wild boar inhabiting this area might pose a real threat to local livestock and many of the protected species (for example European bison that can share feeding places with wild boar). Given this situation, ongoing monitoring of the prevalence of TB should be conducted, and protective measures should be considered.

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Section: Discussionmentioning

confidence: 99%

Epidemiological characterization of Mycobacterium caprae strains isolated from wildlife in the Bieszczady Mountains, on the border of Southeast Poland

et al. 2020

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“…The occurrence of M. caprae infections is mostly reported in continental Europe, especially in Spain and in countries with alpine wildlife habitats (Domogalla et al, ). Besides red deer and cattle, M. caprae was detected in dromedary camels and bisons in a zoological garden in Slovenia (Pate et al, ), as well as in free‐ranging European bisons in Poland (Krzysiak et al, ), wild boars in Hungary and Spain (Csivincsik, Rónai, Nagy, Svéda, & Halász, ; García‐Jiménez et al, ), and grey wolves in Poland (Orłowska et al, ). Recently, an M. caprae infection outside Europe was reported by Yoshida et al () in a Borneo elephant in Japan.…”

Section: Discussionmentioning

confidence: 99%

Shedding of Mycobacterium caprae by wild red deer ( Cervus elaphus ) in the Bavarian alpine regions, Germany

Dorn‐In¹,

Körner²,

Büttner³

et al. 2019

Transbound Emerg Dis

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The number of natural infections with Mycobacterium caprae in wildlife and in cattle in the Bavarian and Austrian alpine regions has increased over the last decade. Red deer (Cervus elaphus) have been recognized as maintenance reservoir; however, the transmission routes of M. caprae among and from naturally infected red deer are unknown. The unexpected high prevalence in some hot spot regions might suggest an effective indirect transmission of infection. Therefore, this study was undertaken to diagnose the occurrence of M. caprae in faeces and secretions of red deer in their natural habitat. A total of 2,806 red deer hunted in this region during 2014–2016 were included in this study. After pathological examination, organs (lymph nodes, lung, heart), excretions and secretions (faeces, urine, saliva and tonsil swabs) were further investigated by qPCR specific for Mycobacterium tuberculosis complex (MTC), M. bovis and M. caprae. Samples tested positive by qPCR were processed for culturing of mycobacteria. In total, 55 (2.0%) animals were confirmed positive for M. caprae by pathological examination, PCR and culturing of the affected organ material. With the exception of one sample, all of the secretion and excretion samples were negative for mycobacteria of the Mycobacterium tuberculosis complex (MTC). From one red deer, M. caprae could be isolated from the heart sac as well as from the faeces. Whole‐genome sequencing confirmed that both strains were clonally related. This is the first confirmation that M. caprae can be shed with the faeces of a naturally infected red deer. However, further studies focusing on a higher number of infected animals, sample standardization and coordinated multiple sampling are necessary to improve the understanding of transmission routes under natural conditions.

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“…Gruźlica bydła (bovine tuberculosis, bTB, BTB) jest powolnie postępującą chorobą zakaźną spowodowaną przez kwasooporne prątki bydlęce: Mycobacterium bovis oraz Mycobacterium caprae, które należą do kompleksu Mycobacterium tuberculosis (MTBC) (27). Prątki bydlęce mogą być również czynnikiem etiologicznym gruźlicy u innych gatunków zwierząt gospodarskich, zwierząt wolno żyjących i ludzi (13,20,22,24). Gruźlica bydła generuje znaczne obciążenia ekonomiczne i zdrowotne, co uzasadnia rygorystyczne badania epidemiologiczne dotyczące monitorowania i kontroli tej zoonozy.…”

Section: Praca Oryginalna Original Paperunclassified

Molecular characterization of Mycobacterium tuberculosis complex strains isolated from cattle in Masovian Voivodeship in 2008-2012

Krajewska‐Wędzina¹,

Kozińska²,

Augustynowicz–Kopeć³

et al. 2018

Medycyna Weterynaryjna

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Since 2009, Poland has had a TB-free status, although over the last seven years 12-34 cases of bovine TB have been recorded annually. In 2009-2012 the largest number of cattle infected with Mycobacterium bovis and Mycobacterium caprae were culled in Masovian Voivodeship. Likewise, the largest number of sources of this zoonosis were recorded in that voivodeship. The vicinity of farms where bTB was found indicated that it could have been transmitted between their herds. The aim of this study was to characterise the molecular patterns of bovine bacillus strains isolated from cattle in Masovian Voivodeship and the molecular relationships between them. The material for microbiological examination came from 38 cattle (Bos taurus) located in 7 counties of Masovian Voivodeship. These 38 strains of MTBC were further identified as M. bovis (24 isolates; 63%) and M. caprae (14 isolates; 37%). A two-step genotyping analysis of the 38 MTBC strains identified 24 molecular patterns, closely related phylogenetically, which were assigned to 8 clusters of 2-6 strains. Sources of transmission were identified in 8 out of 13 herds examined in the 7 counties of Masovian Voivodeship. The results of the genotyping analysis excluded the possibility of TB transmission between different herds in Masovian Voivodeship. It was proved, however, that TB had been transmitted between animals bred on one of the farms..

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Mycobacterium caprae transmission to free-living grey wolves (Canis lupus) in the Bieszczady Mountains in Southern Poland

Cited by 23 publications

References 15 publications

Epidemiological characterization of Mycobacterium caprae strains isolated from wildlife in the Bieszczady Mountains, on the border of Southeast Poland

Epidemiological characterization of Mycobacterium caprae strains isolated from wildlife in the Bieszczady Mountains, on the border of Southeast Poland

Shedding of Mycobacterium caprae by wild red deer ( Cervus elaphus ) in the Bavarian alpine regions, Germany

Molecular characterization of Mycobacterium tuberculosis complex strains isolated from cattle in Masovian Voivodeship in 2008-2012

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