Mycobacterium bovis infection at the interface between domestic and wild animals in Zambia

Hang’ombe, Mudenda Bernard; Munyeme, Musso; Nakajima, Chie; Fukushima, Yukari; Suzuki, Haruka; Matandiko, Wigganson; Ishii, Akihiro; Mweene, Aaron S.; Suzuki, Yasuhiko

doi:10.1186/1746-6148-8-221

Cited by 24 publications

(21 citation statements)

References 39 publications

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“…MIRU10, MIRU20, MIRU26 and QUB3336 had similar results in studies on M. bovis isolated from Xinjiang region [16]. The published data on M. tuberculosis from Ghana [24] and other locations [6,13,14] showed higher allelic diversities than our study, which suggests that not all MIRU-VNTR loci are informative for M. bovis strains form Sika Deer in China.…”

Section: Discussionsupporting

confidence: 85%

Evaluation of MIRU-VNTR for typing of Mycobacterium bovis isolated from Sika deer in Northeast China

Yang

Wang

et al. 2015

BMC Vet Res

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BackgroundBovine tuberculosis has led to serious economic losses for Sika Deer producers in China. Strategies for controlling the spread of Mycobacterium bovis are often hampered by a lack of epidemiological data. Specifically, tracing infections requires the ability to trace back infections, which, in turn, requires the ability to determine isolates with a common source. This study was planned to assess the discriminatory power of each mycobacterial interspersed repetitive unit (MIRU)-variable number tandem repeats (VNTR) locus and evaluate the most appropriate combination of MIRU-VNTR loci for molecular epidemiological studies on Sika Deer in China.ResultsThe discriminatory power of MIRU-VNTR typing based on 22 known loci (12 MIRUs, 2 ETRs, 4 QUBs, and 4 Mtubs) were assessed in 96 Mycobacterium bovis strains collected sequentially from Sika Deer at a slaughterhouse in northeastern China. We defined four loci (MIRU4, ETRA, QUB11b, and Mtub4) as highly discriminative, eight loci (MIRU2, MIRU23, MIRU27, MIRU31, MIRU39, MIRU40, QUB26, and Mtub21) as moderately discriminative, and three loci (MIRU16, Mtub30, and Mtub34) as poorly discriminative. The final locus showed no polymorphism between strains. MIRU-VNTR typing as a whole was highly discriminative, with an overall allelic diversity of 0.897. Of the loci tested, the four highly discriminative loci and eight moderately discriminative loci proved to be most appropriate for first line typing of M. bovis from Sika Deer, with the same resolving ability as all 22 loci (H = 0.897).ConclusionsMIRU-VNTR typing is quick and effective for typing bovine tuberculosis isolates from Sika Deer in China.Electronic supplementary materialThe online version of this article (doi:10.1186/s12917-015-0402-0) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 85%

Evaluation of MIRU-VNTR for typing of Mycobacterium bovis isolated from Sika deer in Northeast China

Yang

Wang

et al. 2015

BMC Vet Res

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“…As a proxy for phylogenetic reliability of each tree, the retention index (RI; Farris 1989) was used to assess the fit of the character to “sampling locality” (continents used as tip states) across the 38-genome data set, following previous conclusions showing that regionally M. bovis samples tend to cluster together (e.g., Hang’ombe et al. 2012; Allen et al.…”

Section: Methodsmentioning

confidence: 99%

Patterns and Processes of Mycobacterium bovis Evolution Revealed by Phylogenomic Analyses

Patané

Martins

Castelão

et al. 2017

Genome Biology and Evolution

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Mycobacterium bovis is an important animal pathogen worldwide that parasitizes wild and domesticated vertebrate livestock as well as humans. A comparison of the five M. bovis complete genomes from the United Kingdom, South Korea, Brazil, and the United States revealed four novel large-scale structural variations of at least 2,000 bp. A comparative phylogenomic study including 2,483 core genes of 38 taxa from eight countries showed conflicting phylogenetic signal among sites. By minimizing this effect, we obtained a tree that better agrees with sampling locality. Results supported a relatively basal position of African strains (all isolated from Homo sapiens), confirming that Africa was an important region for early diversification and that humans were one of the earliest hosts. Selection analyses revealed that functional categories such as “Lipid transport and metabolism,” “Cell cycle control, cell division, chromosome partitioning” and “Cell motility” were significant for the evolution of the group, besides other categories previously described, showing importance of genes associated with virulence and cholesterol metabolism in the evolution of M. bovis. PE/PPE genes, many of which are known to be associated with virulence, were major targets for large-scale polymorphisms, homologous recombination, and positive selection, evincing for the first time a plethora of evolutionary forces possibly contributing to differential adaptability in M. bovis. By assuming different priors, US strains originated and started to diversify around 150–5,210 ya. By further analyzing the largest set of US genomes to date (76 in total), obtained from 14 host species, we detected that hosts were not clustered in clades (except for a few cases), with some faster-evolving strains being detected, suggesting fast and ongoing reinfections across host species, and therefore, the possibility of new bovine tuberculosis outbreaks.

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“…Lechwe, an antelope in Zambia, is another reservoir host of M. bovis that contributes to the spread of the infection to livestock [33]. Furthermore, Zambia has other flagship wildlife species such as elephants, lions, and hippos, to name a few, but the situation of TB in these species has yet to be ascertained.…”

Section: Global Exposition Of Wildlife Managementmentioning

confidence: 99%

Wildlife Tuberculosis: An Emerging Threat for Conservation in South Asia

Thapa¹,

Nakajima²,

Gairhe³

et al. 2017

Global Exposition of Wildlife Management

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Wildlife tuberculosis (TB) is becoming one of the emerging challenges for conservation globally. South Asian region is home to many endangered species like Asian elephants, rhinoceros, and Bengal tigers. Although it carries more than one-third of global burden of human TB, TB in livestock and wildlife has not been adequately studied. This chapter reviews the present knowledge and information about animal-adapted members of Mycobacterium tuberculosis complex and wildlife TB in South Asia. Recent studies of TB from different wild animals in Nepal and Bangladesh have found that M.orygis is an emerging threat of wildlife TB in the region. These studies have demonstrated wide diversity of M. orygis strains circulating in the region indicating its endemic distribution. M. orygis-associated TB was discovered from a free-ranging rhinoceros in Nepal and the finding could signify threat of TB in other wild animals, including a possibility of unknown maintenance host. Recent studies also revealed an emerging challenge caused by TB to elephants in different South Asian countries like Nepal, India, and Sri Lanka.Wildlife TB is becoming a conservation challenge in South Asia, but given the paucity of research in this area, it is overlooked and underexplored.

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Mycobacterium bovis infection at the interface between domestic and wild animals in Zambia

Cited by 24 publications

References 39 publications

Evaluation of MIRU-VNTR for typing of Mycobacterium bovis isolated from Sika deer in Northeast China

Evaluation of MIRU-VNTR for typing of Mycobacterium bovis isolated from Sika deer in Northeast China

Patterns and Processes of Mycobacterium bovis Evolution Revealed by Phylogenomic Analyses

Wildlife Tuberculosis: An Emerging Threat for Conservation in South Asia

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