Mycobacterium avium subsp. hominissuis infection in two sibling Fjord horses diagnosed using quantitative real time PCR: a case report

Blahutkova, M.; Fictum, P.; Škorič, M.; Bezděková, B.; Jahn, P.; Kříž, P.; Mrlík, V.; Slaná, Iva; Kaevska, Marija; Pavlík, I.

doi:10.17221/1544-vetmed

Cited by 10 publications

(8 citation statements)

References 35 publications

(39 reference statements)

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“…Ky33 profile has been isolated in European countries, but not isolated in Japan. This profile has been isolated from pig, and cattle in France [25], and pig and chicken in Finland [3]. These inconsistent results may be due to the age of pigs.…”

Section: Discussionmentioning

confidence: 99%

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Genotyping of swine Mycobacterium avium subsp. hominissuis isolates from Kyushu, Japan

Subangkit

Yamamoto

Ishida

et al. 2019

The Journal of Veterinary Medical Science

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The incidence of diseases caused by nontuberculous mycobacteria (NTM) is increasing annually worldwide, including Japan. Mycobacterium avium subsp. hoiminissuis (MAH) is one of the most common NTM species responsible for chronic lung diseases in animals and humans. In the current study, mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) typing was employed to characterize the genetic diversity of swine MAH isolates from Kyushu, Japan. In total, 309 isolates were obtained from the lymph nodes of 107 pigs not displaying any clinical signs of disease, of which 307 were identified as MAH, comprising 173 strains. Based on eight established MIRU-VNTR loci, the MAH strains represented 50 genotypes constituting three lineages, and 29 had not been described in the Mac French National Institute for Agricultural Research Nouzilly MIRU-VNTR (Mac-INMV) database. MAH was the dominant M. avium complex (MAC) in pigs from Kyushu, and there was high genetic diversity among genotype profiles of MAH from Kyushu. We identified three predominant genotype profiles in the tested area sharing high relatedness with genotype profiles of strains isolated in European countries. MAH was the most common NTM in pigs from Kyushu and exhibited high diversity, with new strain-derived genotypes.

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Section: Discussionmentioning

confidence: 99%

“…MAH infection in pigs is characterized by granulomatous lesions in the lymph nodes of the digestive system [3]. Since most pigs do not show any clinical signs of infection, visceral organs, including lesions, are excluded from processing by macroscopic inspection at the slaughterhouse.…”

mentioning

confidence: 99%

Genotyping of swine Mycobacterium avium subsp. hominissuis isolates from Kyushu, Japan

Subangkit

Yamamoto

Ishida

et al. 2019

The Journal of Veterinary Medical Science

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“…In available literature, there are no reports of clinicopathological findings associated with the M. bovis infection in horses, but it has been reported that Mycobacterium avium hominissuis (the causative agent of avian mycobacteriosis) infection in horses led to thrombocytosis, hypoalbuminemia, increased serum activities of alkaline phosphatase and lactate dehydrogenase, decreased packed cell volume and presence of band neutrophil in peripheral blood (Blahutkova et al, 2011). M. ulcerans infection in horses led to a mild decrease in the serum levels of sodium, creatinine and inorganic phosphate and increased serum activity of gamma glutamyl transferase (Van Zyl et al, 2010).…”

Section: Tuberculosismentioning

confidence: 99%

Haematology and Clinical Biochemistry Findings Associated with Equine Diseases - a Review

Agina

2017

Not Sci Biol

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The course and outcome of a disease process is dependent upon factors such as the disease-causing agent and its cell tropism, defense mechanisms of the host, genetic resistance of the species or breed affected, as well as the age, nutritional status and hormonal levels of the affected animal. When haematology, clinical biochemistry and cytology test results are combined with other laboratory procedures, complete physical examination and also with the history of the patient, a veterinarian is well armed to arrive at a definitive diagnosis, make a certain prognosis (good, poor or guarded) and can also make a concluding statement on the efficacy of the instituted therapy. In clinical biochemistry, demonstration of specific enzyme activity and concentration of analytes in serum/plasma facilitates the disease diagnosis. Also, evaluation of haematology, clinical biochemistry and diagnostic cytology tests can help establish the presence or absence of diseases of internal organs, and by serial performance of these tests, may help to determine whether a disease process remains static, progressive or regressive. This review therefore provides the haematological, serum biochemical and cytological characteristics of diseases caused by the main bacteria, viruses, fungi, protozoa, helminths, arthropods, nutritional deficiencies, endocrine disturbances, neoplasm, allergy, toxins (phytoxins and zootoxins) and inorganic poisons in horses.

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“…; Blahutkova et al . ). Mycobacteria have been identified from a liver biopsy of a horse with diarrhoea and weight loss (Lofstedt and Jakowski ).…”

Section: Introductionmentioning

confidence: 97%

“…Molecular identification of mycobacterial agents from diseased intestine using polymerase chain reaction (PCR) is diagnostic (Pavlik et al 2008;Ryhner et al 2009;Keck et al 2010), and serology can also be used to identify a rising antibody titre to mycobacteria (Mair 1996). Intestinal mycobacteriosis manifests as a gastrointestinal syndrome but at post mortem examination the disease is often disseminated (Cline et al 1991;Blahutkova et al 2011). Mycobacteria have been identified from a liver biopsy of a horse with diarrhoea and weight loss (Lofstedt and Jakowski 1989).…”

Section: Introductionmentioning

confidence: 99%

Disseminated alimentary mycobacteriosis in the horse: a retrospective study of nine cases

Mönki

Hewetson

Hahn

et al. 2015

Equine Veterinary Education

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Summary This paper summarises the clinical findings of 9 cases of disseminated alimentary mycobacteriosis in horses presented at a Finnish referral equine hospital 2009–2014. Four of 9 horses were Standardbreds and 8/9 horses were male. The median age was 2 years, ranging from 6 months to 15 years. The duration of clinical signs before admission ranged from 2 weeks to 6 months. All horses demonstrated deterioration of the clinical signs after a protracted period of the disease and were finally subjected to euthanasia after poor response to multiple medical therapies. The most common complaints on admission were weight loss and diarrhoea (9/9), pyrexia (7/9), ventral oedema (7/9), lethargy (7/9) and inappetance (6/9). The most common clinicopathological abnormalities were hypoalbuminaemia and hyperfibrinogenaemia, which were present in all horses. Rectal biopsy specimens were examined from 5/9 horses and specimens were stained with Ziehl‐Nielsen (ZN). At rectal biopsy, mild multifocal neutrophilic or mild granulomatous proctitis was recognised in all 5 horses, but the ZN stain for mycobacteria was positive in only one biopsy. A liver biopsy was taken from one horse in which hepatomegaly was observed clinically and revealed marked granulomatous hepatitis with the presence of mycobacteria. The rectal biopsy from this horse was ZN negative. At post mortem examination, chronic, multifocal to coalescing granulomatous typhlocolitis and lymphadenitis were found in all horses with the small intestine less frequently involved. At histopathological examination of post mortem samples, a ZN stain was performed and intracellular acid‐fast bacilli were identified in macrophages and multinucleated giant cells in the large intestine, liver and lymph nodes in 9/9 horses and in the small intestine in 5/9 horses. Mycobacterium avium ssp. hominissuis was isolated in 5/9 horses from post mortem samples.

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Mycobacterium avium subsp. hominissuis infection in two sibling Fjord horses diagnosed using quantitative real time PCR: a case report

Cited by 10 publications

References 35 publications

Genotyping of swine <i>Mycobacterium avium</i> subsp. <i>hominissuis</i> isolates from Kyushu, Japan

Genotyping of swine <i>Mycobacterium avium</i> subsp. <i>hominissuis</i> isolates from Kyushu, Japan

Haematology and Clinical Biochemistry Findings Associated with Equine Diseases - a Review

Disseminated alimentary mycobacteriosis in the horse: a retrospective study of nine cases

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