Mycobacterium avium ssp . paratuberculosis detection in animals, food, water and other sources or vehicles of human exposure: A scoping review of the existing evidence

Waddell, Lisa; Rajić, Andrijana; Stärk, Katharina D.C.; McEwen, Scott A.

doi:10.1016/j.prevetmed.2016.08.003

Cited by 49 publications

(41 citation statements)

References 132 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…paratuberculosis DNA was present in 4% of dairy foods. This figure is consistent with the lowest percentages of positives reported elsewhere (Grant, 2010; Waddell et al, 2016). In spite of this, we found less M .…”

Section: Discussionsupporting

confidence: 92%

“…avium subsp. paratuberculosis from raw and pasteurized milk products (Waddell et al, 2016), culture did not produce any isolates. Our survey did not include any raw milk products and the number of pasteurized liquid milk samples was reduced.…”

Section: Discussionmentioning

confidence: 98%

“…There is a lack of information with reference to supermarket foods that have passed the established controls. Many studies report on the detection of mycobacteria in animal tissues and raw milk and thus support that foods of animal origin may act as vehicles of mycobacteria transmission to humans (Kaneene et al, 2014; Klanicova-Zalewska and Slana, 2014; Pérez-Lago et al, 2014; Waddell et al, 2016). Several surveys have found mycobacterial DNA and viable cells in commercially available foodstuffs but the number of such reports is limited and most of them are mainly focused on the detection of M .…”

Section: Introductionmentioning

confidence: 96%

See 2 more Smart Citations

Detection of Mycobacteria by Culture and DNA-Based Methods in Animal-Derived Food Products Purchased at Spanish Supermarkets

et al. 2017

View full text Add to dashboard Cite

Mycobacteria include obligate and opportunistic pathogens that cause significant human and animal disease. The burden of tuberculosis has been largely reduced in developed territories but remains a huge problem worldwide. The significance of nontuberculous mycobacteria is growing considerably, especially in developed regions with higher life expectancy and more therapy-related immunosuppressed individuals. Due to their robustness mycobacteria can contaminate animal products by direct transmission from infected individuals or by environmental contamination during processing. The situation at market level is poorly known. Most studies analyzing commercially available foods are limited to a small or local scale and mainly focused on a particular mycobacterial species. There is a need to investigate if animal products that have passed the established controls to be for sale at main supermarkets could represent a route of contact with any mycobacteria. Thus, our goal was to study the prevalence of mycobacteria in these foods to assess if this could represent a source of human exposure. Five stores from the main supermarket chains in Spain were selected. 138 dairy and 119 meat products were purchased. All were processed using culture and multiplex real-time PCR methods. Additional molecular methods were used to specifically identify any positive result. Mycobacterium avium subsp. hominissuis (2), M. avium subsp. avium (1), and M. fortuitum (1) were isolated from powdered infant formula and ground beef, chicken sausage, and mortadella cold cut, respectively. Mycobacterial DNA (M. avium, M. tuberculosis complex and other nontuberculous mycobacteria) was detected in 15% of dairy products and 2% of meat products. These results show that the prevalence of viable mycobacteria in foods of animal origin obtained at the supermarket was not substantial although a considerable proportion of them contained mycobacterial DNA. Contact with mycobacteria through this route could be ensured over time. Further investigation is necessary to determine the real impact of foodborne mycobacterial exposure on human health and identify critical points in the food production system to enable setting up more stringent control measures.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 96%

See 1 more Smart Citation

Detection of Mycobacteria by Culture and DNA-Based Methods in Animal-Derived Food Products Purchased at Spanish Supermarkets

et al. 2017

View full text Add to dashboard Cite

show abstract

“…The result of this study proposes that pasteurized milk would need to be considered as a weak transmission vector confirming the previous report [48] and is in line with other reports. Data regarding the intake of dairy products were not available for the enrolled patients; consumption of processed cheese has been significantly associated with CD and its evaluation in Iranian cohort would be worthwhile [49]. …”

Section: Discussionmentioning

confidence: 99%

Mycobacterium avium subsp. paratuberculosis and associated risk factors for inflammatory bowel disease in Iranian patients

et al. 2017

View full text Add to dashboard Cite

BackgroundInflammatory bowel disease (IBD) is described as a relapsing condition with high morbidity and uncertain complex pathogenesis. The association of Mycobacterium avium ssp. paratuberculosis (MAP) with Crohn’s disease (CD) in human has been debated for decades, however there is no confirmed data to verify such relations in Iran. The aim of this study was to investigate risk factors and a possible role of MAP in Iranian patients with CD.MethodsAnti-MAP antibodies were detected in serum of IBD patients and subjects without IBD (nIBD) through ELISA; MAP DNA and viable MAP cells were identified in patients’ biopsies through nested PCR and direct culture methods, respectively. Principal component analysis (PCA) was used to investigate the risk factors in relation to IBD and MAP infection.ResultsPositivity for IS900 PCR was detected in 64% (n = 18) of CD, 33% (n = 10) of ulcerative colitis (UC) and 9.7% (n = 6) of nIBD samples. Live MAP cells were isolated from biopsies of 2 CD patients only. Among 28 patients with CD, 46% (n = 13) and 39% (n = 11) were positive for antibodies against MAP3865c133–141 and MAP3865c125–133 peptides, respectively, whereas much lower seroreactivity was detected in UC subjects accounting for 3% (n = 1) for MAP3865c133–141 and 16.7% (n = 5) for MAP3865c125–133. A high immune reactivity to MAP epitopes among CD patients was positively correlated with consumption of fast food meals and IBD familiarity. For both CD and UC, breastfeeding period and consumption of fruit/vegetables presented negative correlation with the presence of anti-MAP antibodies.ConclusionsThis study provided evidences that high prevalence of MAP DNA and anti-MAP antibodies in CD patients is significantly associated with the development of CD. Despite the role of several factors contributing to IBD, the presence of MAP DNA and anti-MAP antibodies in Iranian CD patients highlights a possible transmission of MAP from animal-derived products to humans.

show abstract

“…JD is a chronic wasting disease affecting cattle, sheep, goats and other ruminants caused by M. avium subspecies paratuberculosis ( Map) (Harris & Barletta, 2001). Rabbit, deer and other wildlife have also been shown to be infected by Map (Waddell et al, 2016). While various species of wildlife can act as a reservoir of Map (Nugent et al, 2011), the clinical symptoms of JD have only been observed in ruminants (Koets, Eda & Sreevatsan, 2015), camelids (Ghosh et al, 2012) and rabbits/hare (Beard et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Examination ofMycobacterium aviumsubspeciesparatuberculosismixed genotype infections in dairy animals using a whole genome sequencing approach

Davidson

Ahlstrom

Buck

et al. 2016

PeerJ

View full text Add to dashboard Cite

Many pathogenic mycobacteria are known to cause severe disease in humans and animals. M. avium subspecies paratuberculosis (Map) is the causative agent of Johne’s disease—a chronic wasting disease affecting ruminants such as cattle and sheep, responsible for significant economic losses in the dairy and beef industries. Due to the lack of treatment options or effective vaccines, mitigating losses can be difficult. In addition, the early stages of Map infection may occur in asymptomatic hosts that continue to shed viable bacteria in their faeces, leading to the infection of other healthy animals. Using multi-locus short sequence repeat (ML-SSR) analysis we previously reported that individual Johne’s positive dairy cattle from farms across the island of Newfoundland were infected by Map with multiple SSR-types simultaneously. The occurrence of multiple mixed genotype infections has the potential to change pathogen and disease dynamics as well as reduce the efficacy of treatments and vaccines. Therefore, we conducted whole genome sequencing (WGS) and single nucleotide polymorphism (SNP) analysis on a subset of these isolates for a more in-depth examination. We also implemented a PCR assay using two discriminatory SNPs and demonstrated the incidence of a mixed infection by three genotypically diverse Map isolates in a single animal. In addition, results show that WGS and SNP analysis can provide a better understanding of the relationship between Map isolates from individual and different animals. In the future such studies on the occurrence of mixed genotype infections could potentially lead to the identification of variable pathogenicity of different genotypes and allow for better tracking of Map isolates for epidemiological studies.

show abstract

Mycobacterium avium ssp . paratuberculosis detection in animals, food, water and other sources or vehicles of human exposure: A scoping review of the existing evidence

Cited by 49 publications

References 132 publications

Detection of Mycobacteria by Culture and DNA-Based Methods in Animal-Derived Food Products Purchased at Spanish Supermarkets

Detection of Mycobacteria by Culture and DNA-Based Methods in Animal-Derived Food Products Purchased at Spanish Supermarkets

Mycobacterium avium subsp. paratuberculosis and associated risk factors for inflammatory bowel disease in Iranian patients

Examination ofMycobacterium aviumsubspeciesparatuberculosismixed genotype infections in dairy animals using a whole genome sequencing approach

Contact Info

Product

Resources

About