Mycobacterium africanum Is Associated with Patient Ethnicity in Ghana

Asante-Poku, Adwoa; Yeboah-Manu, Dorothy; Otchere, Isaac Darko; Aboagye, Samuel Yaw; Stucki, David; Hattendorf, Jan; Borrell, Sònia; Feldmann, Julia; Gagneux, Sébastien

doi:10.1371/journal.pntd.0003370

Cited by 64 publications

(79 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, two independent studies conducted by our group found a strong association between L5 and an ethnic group in Ghana [[25], Asante-Poku et al , submitted].…”

Section: Discussionmentioning

confidence: 92%

Spatio-Temporal Distribution of Mycobacterium tuberculosis Complex Strains in Ghana

et al. 2016

Self Cite

View full text Add to dashboard Cite

BackgroundThere is a perception that genomic differences in the species/lineages of the nine species making the Mycobacterium tuberculosis complex (MTBC) may affect the efficacy of distinct control tools in certain geographical areas. We therefore analyzed the prevalence and spatial distribution of MTBC species and lineages among isolates from pulmonary TB cases over an 8-year period, 2007–2014.MethodologyMycobacterial species isolated by culture from consecutively recruited pulmonary tuberculosis patients presenting at selected district/sub-district health facilities were confirmed as MTBC by IS6110 and rpoß PCR and further assigned lineages and sub lineages by spoligotyping and large sequence polymorphism PCR (RDs 4, 9, 12, 702, 711) assays. Patient characteristics, residency, and risks were obtained with a structured questionnaire. We used SaTScan and ArcMap analyses to identify significantly clustered MTBC lineages within selected districts and spatial display, respectively.ResultsAmong 2,551 isolates, 2,019 (79.1%), 516 (20.2%) and 16 (0.6%) were identified as M. tuberculosis sensu stricto (MTBss), M. africanum (Maf), 15 M. bovis and 1 M. caprae, respectively. The proportions of MTBss and Maf were fairly constant within the study period. Maf spoligotypes were dominated by Spoligotype International Type (SIT) 331 (25.42%), SIT 326 (15.25%) and SIT 181 (14.12%). We found M. bovis to be significantly higher in Northern Ghana (1.9% of 212) than Southern Ghana (0.5% of 2339) (p = 0.020). Using the purely spatial and space-time analysis, seven significant MTBC lineage clusters (p< 0.05) were identified. Notable among the clusters were Ghana and Cameroon sub-lineages found to be associated with north and south, respectively.ConclusionThis study demonstrated that overall, 79.1% of TB in Ghana is caused by MTBss and 20% by M. africanum. Unlike some West African Countries, we did not observe a decline of Maf prevalence in Ghana.

show abstract

“…Moreover, two independent studies conducted by our group found a strong association between L5 and an ethnic group in Ghana [[25], Asante-Poku et al , submitted].…”

Section: Discussionmentioning

confidence: 92%

Spatio-Temporal Distribution of Mycobacterium tuberculosis Complex Strains in Ghana

et al. 2016

Self Cite

View full text Add to dashboard Cite

show abstract

“…Humans differ in their susceptibility to TB39, and human genetic diversity may thus determine the width of the ecological niche accessible to different MTBC genotypes40,41. The geographical restriction of particular MTBC genotypes might reflect local adaption of these pathogen variants to the corresponding human host populations13,15.…”

Section: Resultsmentioning

confidence: 99%

Mycobacterium tuberculosis lineage 4 comprises globally distributed and geographically restricted sublineages

et al. 2016

Self Cite

View full text Add to dashboard Cite

Generalist and specialist species differ in the breadth of their ecological niche. Little is known about the niche width of obligate human pathogens. Here we analyzed a global collection of Mycobacterium tuberculosis Lineage 4 clinical isolates, the most geographically widespread cause of human tuberculosis. We show that Lineage 4 comprises globally distributed and geographically restricted sublineages, suggesting a distinction between generalists and specialists. Population genomic analyses showed that while the majority of human T cell epitopes were conserved in all sublineages, the proportion of variable epitopes was higher in generalists. Our data further support a European origin for the most common generalist sublineage. Hence, the global success of Lineage 4 reflects distinct strategies adopted by different sublineages and the influence of human migration.

show abstract

“…Notably, a Swiss cohort study found that MTBC lineages tend to transmit preferentially among sympatric host populations[34]. Two other studies in Ghana offered a potential explanation for the geographical restriction of lineage 5 in West Africa, by showing association between these lineages and specific human ethnicities[35, 36]. A dominant lineage model of evolution can also help explain the various clinical outcomes resulting from infections with strains from distinct lineages of the MTBC.…”

Section: Discussionmentioning

confidence: 99%

Within Host Evolution Selects for a Dominant Genotype of Mycobacterium tuberculosis while T Cells Increase Pathogen Genetic Diversity

et al. 2016

Self Cite

View full text Add to dashboard Cite

Molecular epidemiological assessments, drug treatment optimization, and development of immunological interventions all depend on understanding pathogen adaptation and genetic variation, which differ for specific pathogens. Mycobacterium tuberculosis is an exceptionally successful human pathogen, yet beyond knowledge that this bacterium has low overall genomic variation but acquires drug resistance mutations, little is known of the factors that drive its population genomic characteristics. Here, we compared the genetic diversity of the bacteria that established infection to the bacterial populations obtained from infected tissues during murine M. tuberculosis pulmonary infection and human disseminated M. bovis BCG infection. We found that new mutations accumulate during in vitro culture, but that in vivo, purifying selection against new mutations dominates, indicating that M. tuberculosis follows a dominant lineage model of evolution. Comparing bacterial populations passaged in T cell-deficient and immunocompetent mice, we found that the presence of T cells is associated with an increase in the diversity of the M. tuberculosis genome. Together, our findings put M. tuberculosis genetic evolution in a new perspective and clarify the impact of T cells on sequence diversity of M. tuberculosis.

show abstract

Mycobacterium africanum Is Associated with Patient Ethnicity in Ghana

Cited by 64 publications

References 35 publications

Spatio-Temporal Distribution of Mycobacterium tuberculosis Complex Strains in Ghana

Spatio-Temporal Distribution of Mycobacterium tuberculosis Complex Strains in Ghana

Mycobacterium tuberculosis lineage 4 comprises globally distributed and geographically restricted sublineages

Within Host Evolution Selects for a Dominant Genotype of Mycobacterium tuberculosis while T Cells Increase Pathogen Genetic Diversity

Contact Info

Product

Resources

About