Mycobacteriosis in zebrafish (Danio rerio) research facilities

Kent, Michael L.; Whipps, Christopher M.; Matthews, Jennifer L.; Florio, Daniela; Watral, Virginia; Bishop-Stewart, Janell K.; Poort, Melanie J.; Bermudez, Luiz E.

doi:10.1016/j.cca.2004.08.005

Cited by 84 publications

(154 citation statements)

References 23 publications

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“…Fish mycobacteriosis, caused by M. peregrinum, has been previously reported in cultured Pacific white shrimp (Penaeus vannamei) (18) and in fish (2,13,22). A very rare case of M. peregrinum infection in healthy freshwater aquarium fish was reported.…”

Section: Discussionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

“…Fish mycobacteriosis is well-recognised in marine and freshwater fish, with several reviews concerning the topic (6,7,12 (1,7,13,32). Environmental mycobacteria can be pathogenic to a variety of freshwater and marine fish species both in the wild and kept in captivity (15,21) (3,13,20,22,29). Clinical signs of fish mycobacteriosis are quite variable and usually include loss of appetite, lethargy, staying alone in the corner of the aquarium, floating on the surface of the water, anorexia, skin discoloration, open lesions, ulcerations, unilateral or bilateral exophthalmia, emaciation, skeletal deformities, and well-demarcated granulomas in internal organs (7,25).…”

Section: Introductionmentioning

confidence: 99%

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Occurrence and antimicrobial susceptibility of Mycobacterium peregrinum in ornamental fish

Guz

Grądzki

Krajewska

et al. 2013

Bulletin of the Veterinary Institute in Pulawy

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Section: Discussionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Occurrence and antimicrobial susceptibility of Mycobacterium peregrinum in ornamental fish

Guz

Grądzki

Krajewska

et al. 2013

Bulletin of the Veterinary Institute in Pulawy

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“…35 M. peregrinum is relatively uncommon and is usually subclinical, 36 although a high mortality epizootic has also been reported in association with this species. 37,38 Mycobacterium spp. have been detected in embryos and larvae, as well as adults.…”

Section: Choice Of Agentsmentioning

confidence: 99%

Recommendations for Health Monitoring and Reporting for Zebrafish Research Facilities

2016

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The presence of subclinical infection or clinical disease in laboratory zebrafish may have a significant impact on research results, animal health and welfare, and transfer of animals between institutions. As use of zebrafish as a model of disease increases, a harmonized method for monitoring and reporting the health status of animals will facilitate the transfer of animals, allow institutions to exclude diseases that may negatively impact their research programs, and improve animal health and welfare. All zebrafish facilities should implement a health monitoring program. In this study, we review important aspects of a health monitoring program, including choice of agents, samples for testing, available testing methodologies, housing and husbandry, cost, test subjects, and a harmonized method for reporting results. Facilities may use these recommendations to implement their own health monitoring program.

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“…Based on genotypic and phenotypic findings, it is proposed that these isolates represent a novel species of the genus Mycobacterium, for which the name Mycobacterium stomatepiae sp. nov. is proposed with strain T11 T (5DSM 45059 T 5CIP 109275 T 5NCIMB 14252 T ) as the type strain.In the past few years, the occurrence of fish mycobacteriosis has been increasingly reported (Kent et al, 2004;Pate et al, 2005;Sakai et al, 2005;Whipps et al, 2007b). Although three species, Mycobacterium marinum, Mycobacterium fortuitum and Mycobacterium chelonae, have been frequently cited as the main causative agents of infections in fish, several other mycobacterial species, including a number of novel species, have also been associated with mycobacteriosis (Levi et al, 2003;Rhodes et al, 2003Rhodes et al, , 2005Whipps et al, 2007a).…”

mentioning

confidence: 99%

Mycobacterium stomatepiae sp. nov., a slowly growing, non-chromogenic species isolated from fish

Pourahmad

Cervellione

Thompson

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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Slowly growing, non-chromogenic mycobacteria were isolated from striped barombi mbo cichlids (Stomatepia mariae) maintained at the London Zoo Aquarium, UK. The isolates could be differentiated from other slowly growing, non-pigmented mycobacteria by a combination of phenotypic features including their inability to grow at 37 6C, positive tests for heat-stable catalase, tellurite reduction and arylsulfatase activity, and the absence of urease activity, Tween 80 hydrolysis, nitrate reductase, iron uptake and semiquantitative catalase. The almost full-length 16S rRNA gene sequence, together with partial sequences from the 65 kDa heat-shock protein (hsp65) and the b-subunit of the bacterial RNA polymerase (rpoB) genes and the 16S-23S internal transcribed spacer 1 (ITS 1) region were identical for all three novel strains, but distinct from those of all known mycobacterial species. Phylogenetic analysis based on 16S rRNA gene sequences placed the novel isolates within the slowly growing mycobacteria group in close proximity to Mycobacterium florentinum. Based on genotypic and phenotypic findings, it is proposed that these isolates represent a novel species of the genus Mycobacterium, for which the name Mycobacterium stomatepiae sp. nov. is proposed with strain T11 T (5DSM 45059 T 5CIP 109275 T 5NCIMB 14252 T ) as the type strain.In the past few years, the occurrence of fish mycobacteriosis has been increasingly reported (Kent et al., 2004;Pate et al., 2005;Sakai et al., 2005;Whipps et al., 2007b). Although three species, Mycobacterium marinum, Mycobacterium fortuitum and Mycobacterium chelonae, have been frequently cited as the main causative agents of infections in fish, several other mycobacterial species, including a number of novel species, have also been associated with mycobacteriosis (Levi et al., 2003;Rhodes et al., 2003Rhodes et al., , 2005Whipps et al., 2007a).The majority of slowly growing mycobacteria contain a long helix 18 at position 430-500 (Escherichia coli 16S rRNA gene sequence numbering) in their 16S rRNA gene, which is absent in rapidly growing mycobacteria (Springer et al., 1996a;Tortoli, 2003). There is a third group, with a short helix 18 (due to a deletion of 12 bp), and members of this group are also classified as slow growers (Tortoli, 2003). In this group, Mycobacterium simiae, a photochromogenic organism from monkeys, was the first species to be identified (Weiszfeiler et al., 1981). Mycobacterium intermedium (Meier et al., 1993) is another photochromogenic species related to M. simiae. Scotochromogenic species of the M. simiae-related (MSR) group consist of Mycobacterium interjectum (Springer et al., 1993) The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA, hsp65 and rpoB gene sequences and ITS 1 region of M. stomatepiae sp. nov. T11 T are AM884331, AM902968, AM885873 and AM902938, respectively.Neighbour-joining phylogenetic trees based on hsp65 (Fig. S1) and rpoB ( Fig. S2) gene sequences of M. stomatepiae sp. nov. and selected mycobacterial species, and the oligonucleotides used f...

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Mycobacteriosis in zebrafish (Danio rerio) research facilities

Cited by 84 publications

References 23 publications

Occurrence and antimicrobial susceptibility of Mycobacterium peregrinum in ornamental fish

Occurrence and antimicrobial susceptibility of Mycobacterium peregrinum in ornamental fish

Recommendations for Health Monitoring and Reporting for Zebrafish Research Facilities

Mycobacterium stomatepiae sp. nov., a slowly growing, non-chromogenic species isolated from fish

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