Mycobacteriosis in ornamental fish. Case reports in Sicily and medical-legal considerations

Macrì, D.; Verde, Vincenza Lo; Mancuso, I.; Reale, Stefano; Passantino, Annamaria; Marino, Fabio

doi:10.1007/s11259-008-9157-4

Cited by 5 publications

(3 citation statements)

References 3 publications

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“…[23], unidentified Monogenea species [24], Capillaria spp [25]. and Tetrahymena [26], in addition to bacterial infections caused by Mycobacterium spp [27,28]. and A. hydrophila [29].…”

Section: Discussionmentioning

confidence: 99%

Pathogenesis of mixed infection by Spironucleus sp. and Citrobacter freundii in freshwater angelfish Pterophyllum scalare

Gallani

Sebastião

Valladão

et al. 2016

Microbial Pathogenesis

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The present study was carried out to identify and describe the pathology of the freshwater angelfish Pterophyllum scalare during chronic mortality in an in-door aquaculture system. Scraping of the integument and gills and the collection of intestinal contents to search for external and internal parasites were performed. Kidneys were collected aseptically for the microbiological analysis and the isolates were subjected to antibiotics to test for susceptibility. Subsequently, necropsy for macroscopic assessment and collection of internal organs for histopathology were performed. The fish exhibited lethargy, lip tumor, hemorrhage and liver granuloma. No ectoparasites were diagnosed. Endoparasites of the genus Spironucleus were found in large numbers in the intestine of the affected fish. In the microbiological analysis, Citrobacter freundii was isolated from the kidney and identified by colony PCR. This bacterium showed susceptibility to three of the eight antibiotics evaluated: ciprofloxacin, cefoxitin and tetracycline. For the pathological analysis, liver and spleen granulomas were present. In the intestinal tissue, a large and unusual amount of mast cells and their free granules were described and discussed in detail. The present study showed that mast cells play an important role during the chronic infection of freshwater angelfish.

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“…[23], unidentified Monogenea species [24], Capillaria spp [25]. and Tetrahymena [26], in addition to bacterial infections caused by Mycobacterium spp [27,28]. and A. hydrophila [29].…”

Section: Discussionmentioning

confidence: 99%

Pathogenesis of mixed infection by Spironucleus sp. and Citrobacter freundii in freshwater angelfish Pterophyllum scalare

Gallani

Sebastião

Valladão

et al. 2016

Microbial Pathogenesis

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“…The second most commonly investigated pathogen was Mycobacterium spp. [95,[96][97][98], particularly M. fortuitum [95,[96][97][98] and M. marinum [97]. Prearo et al [96] advocated that Mycobacterium spp.…”

Section: Systematic Reviewmentioning

confidence: 99%

Zoonotic bacteria, antimicrobial use and antimicrobial resistance in ornamental fish: a systematic review of the existing research and survey of aquaculture-allied professionals

et al. 2011

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Using systematic review methodology, global research reporting the frequency of zoonotic bacterial pathogens, antimicrobial use (AMU) and antimicrobial resistance (AMR) in ornamental fish, and human illness due to exposure to ornamental fish, was examined. A survey was performed to elicit opinions of aquaculture-allied personnel on the frequency of AMU and AMR in ornamental fish. The most commonly reported sporadic human infections were associated with Mycobacterium marinum, while Salmonella Paratyphi B var. Java was implicated in all reported outbreaks. Aeromonas spp. were most frequently investigated (n=10 studies) in 25 studies surveying ornamental fish from various sources. High levels of resistance were reported to amoxicillin, penicillin, tetracycline and oxytetracycline, which was also in agreement with the survey respondents' views. Studies on AMU were not found in our review. Survey respondents reported frequent use of quinolones, followed by tetracyclines, nitrofurans, and aminoglycosides. Recommendations for future surveillance and public education efforts are presented.

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“…For example, Gómez (2008) found granulomatous inflammation and numerous acid-fast bacteria in tissue sections of granulomas from a diseased paradise fish, but the causative agent was not confirmed at the species level. Additionally, Marcì et al (2008) reported M. fortuitum infection in severely emaciated paradise fish, with ascites and granulomas of various sizes in the internal organs. In our study, the 16S rRNA and hsp65 gene analysis show that the amplified genes were related most closely to M. marinum.…”

Section: Discussionmentioning

confidence: 99%

Molecular and Histopathological Evidence of Mycobacteriosis in Paradise Fish Macropodus opercularis Imported into Korea

Han¹,

Kim²,

Jeon³

et al. 2013

Fisheries and aquatic sciences

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We report on mycobacteriosis in an imported tropical ornamental fish Macropodus opercularis commonly known as the paradise fish. Mass mortality occurred in paradise fish imported to Korea from Southeast Asia in 2008. The affected fish did not show any outward clinical signs, but enlargement of the spleen, kidneys, and liver was observed on dissection. Histopathological examination revealed numerous granulomas in the spleen, and acid-fast bacilli were observed in the centers of the granulomas. About 65% of spleen DNA samples were PCR positive using mycobacteria-specific primers targeting the 16S rRNA and hsp65 genes. The nucleotide identities of the 16S rRNA and hsp65 genes with those of Mycobacterium marinum were 99.5% and 99.4%, respectively. Although the bacterium was not cultured, the molecular diagnosis and histopathological findings were consistent with mycobacteriosis in paradise fish.

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Mycobacteriosis in ornamental fish. Case reports in Sicily and medical-legal considerations

Cited by 5 publications

References 3 publications

Pathogenesis of mixed infection by Spironucleus sp. and Citrobacter freundii in freshwater angelfish Pterophyllum scalare

Pathogenesis of mixed infection by Spironucleus sp. and Citrobacter freundii in freshwater angelfish Pterophyllum scalare

Zoonotic bacteria, antimicrobial use and antimicrobial resistance in ornamental fish: a systematic review of the existing research and survey of aquaculture-allied professionals

Molecular and Histopathological Evidence of Mycobacteriosis in Paradise Fish Macropodus opercularis Imported into Korea

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