Mycobacterial Topoisomerase I Energetically Suffers From C-Terminal Deletions
Dillon Balthrop,
Deepesh Sigdel,
Chunfeng Mao
et al.
Abstract:Type IA topoisomerases relieve torsional stress in DNA by a strand-passage mechanism, using the strain in the DNA to drive relaxation. The topoisomerase IAs of the Mycobacterium genus have distinct C-terminal domains which are crucial for successful strand-passage. We used single-molecule magnetic tweezers to observe supercoil relaxation by wild typeMycobacterium smegmatistopoisomerase IA and two C-terminal truncation mutants. We recorded distinct behaviors from each truncation mutant. We calculated the free e… Show more
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