Mycobacterial Prevalence and Antibiotic Resistance Frequency Trends in Taiwan of Mycobacterial Clinical Isolates From 2002 to 2014

Abstract

Tuberculosis, caused by Mycobacterium tuberculosis complex (MTBC) infections, is one of the most widespread infectious diseases worldwide. Nontuberculous mycobacteria (NTM) also cause chronic pulmonary infections, however, NTM infection is generally overlooked.This study analyzed the frequencies of MTBC and NTM clinical isolates from 181,132 specimens obtained from patients in Taiwan suspected of having a pulmonary mycobacterial infection from 2002 to 2014. The resistant rates to 4 first-line antibiotics (isoniazid, ethambutol, rifampicin, and streptomycin) of 9079 clinical MTBC isolates were also examined by the modified agar proportion method.Overall, the mycobacterial isolation rate was 8.65%, and this consisted of MTBC isolation rate of 5.01% and NTM isolation rate of 3.63%. The prevalence of MTBC isolates among the identified mycobacterial strains could be seen to decrease significantly from 82.5% in 2002 to 41.18% in 2014. Notably, the corresponding NTM prevalence increased 3.36 fold from 17.54% in 2002 to 58.82% in 2014. The frequencies of MTBC and NTM isolates showed a reciprocal trend with the crossing over occurring in the years 2010 and 2011. Although the resistance rates of the MTBC isolates to isoniazid and streptomycin were relatively stable over the study period, resistance rates of the MTBC isolates against rifampicin and ethambutol fluctuated across the study period. Overall, the incidence of multidrug resistance was relatively consistent at about 1.74%.The diagnosis, identification, and susceptibility tests for NTM should be standardized and integrated into appropriate clinical settings to cope with the increase in NTM infections. In addition, the documentation of the antibiotic resistance rates of MTBC clinical isolates to the antibiotic treatments most often clinically prescribed over a decade provides valuable clues and reference points for effective mycobacterial control.

Role of ethambutol and rifampicin in the treatment of Mycobacterium avium complex pulmonary disease

“…The prevalence of nontuberculous mycobacterial (NTM) pulmonary disease is increasing globally, with the most common pathogen being Mycobacterium avium complex (MAC) species [1–3]. MAC is a ubiquitous organism commonly found in water, dirt, and soil but can cause serious pulmonary diseases in some people.…”

In vitro activity of bedaquiline against Mycobacterium avium complex

“…Special attention should be paid to the study results indicating that the profile of antimicrobial susceptibility for such bacteria as M. tuberculosis varies in the different regions of the world [9, 11, 32, 47–53]. Therefore, it is necessary to accumulate such data in all laboratories where these studies can be performed, since the issues of NTM susceptibility to Bdq have not been fully elucidated so far.…”

Identification of Mycobacterium tuberculosis isolated from culture-negative pulmonary and extra-pulmonary samples in cases of suspected tuberculosis

“…However, due to false positive results and very low specificity and sensitivity, PDD testing is not a suitable method for the detection of PTB and EPTB. In a study conducted in Taiwan in 2016 [14], the frequency of antibiotic-resistant M. tuberculosis was examined. That study was performed on cultured clinical samples collected from 2002 to 2014.…”

“…That study was performed on cultured clinical samples collected from 2002 to 2014. The frequency of culture-positives was 7% in 2002 and about 9% in 2014 [14]. However, the culture method alone cannot be used to precisely determine the prevalence of M. tuberculosis .…”