Mycobacterial interspersed repetitive units (MIRU) differentiate Mycobacterium avium subspecies paratuberculosis from other species of the Mycobacterium avium complex

Bull, Tim J.; Sidi-Boumedine, Karim; McMinn, Elizabeth J.; Stevenson, Karen; Pickup, Roger W.; Hermon‐Taylor, John

doi:10.1016/s0890-8508(03)00047-1

Cited by 80 publications

(94 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Former studies found the presence of a lower number of tandem repeat units at this locus in the Mycobacterium bovis (BCG) and M. avium spp. avium paratuberculosis (316F) vaccine strains in comparison with the rest of the strains tested (Magdalena et al, 1998;Bull et al, 2003). Differences in pathogenesis and progression of the disease probably have a relationship with the variation of tandem repeat units at this particular locus.…”

Section: Discussionmentioning

confidence: 99%

“…report would be related to that described for the M. avium spp. avium reference strain ATCC 25291 (1-7-1-5-5-5) (Bull et al, 2003;Inagaki et al, 2009). Further studies concerning this issue are necessary.…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies have shown the usefulness of tandem repeats or repetitive units to characterize members of the MAC (Bull et al, 2003;Thibault et al, 2007;Mobius et al, 2008). Included within these tandem repeats are the variable number tandem repeats (VNTRs), which are repetitive units dispersed throughout the genome with lengths between 10 and 100 base pairs (Supply et al, 2000); and also the mycobacterial interspersed repetitive units (MIRUs), small interspersed repetitive elements described by Supply et al (1997) in the genome of Mycobacterium tuberculosis.…”

Section: Methodsmentioning

confidence: 99%

“…Isolates of M. avium spp. avium were sub-typed by MIRUÁVNTR with six specific loci previously published: MIRU-1, MIRU-2, MIRU-3, MIRU-4 (Bull et al, 2003), VNTR-25 and VNTR-32 (Thibault et al, 2007;Mobius et al, 2008), using the same specific primers ( Table 2).…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Avian mycobacteriosis in free-living raptors in Majorca Island, Spain

Millán

Negre²,

Castellanos

et al. 2010

Avian Pathology

View full text Add to dashboard Cite

Avian mycobacteriosis is a chronic, infectious disease caused by different species of mycobacteria, usually belonging to the Mycobacterium avium complex. From 2004 to 2007, 589 raptors brought dead or sick to a wildlife rehabilitation centre in Majorca (Balearic Islands, Spain) were necropsied. The birds belonged to 12 different species, chiefly common kestrel (Falco tinnunculus) (n0297), scops owl (Otus scops) (n 0109), barn owl (Tyto alba) (n 075), long-eared owl (Asio otus) (n 058), peregrine falcon (Falco peregrinus) (n 027), and booted eagle (Hieraaetus pennatus) (n013). Gross lesions compatible with mycobacteriosis were observed in 14 birds (2.4%) found in several locations in Majorca. They were 12 kestrels (prevalence in this species, 4.0%), one long-eared owl (1.7%) and one scops owl (0.9%), all the birds presenting whiteÁyellowish nodules from pinpoint size to 1 cm in diameter in diverse organs, mainly in the liver, spleen and intestine. Affected organs were subjected to bacteriology and molecular identification by polymerase chain reaction and, in all cases, infection with M. avium subspecies avium was confirmed. The observed prevalences are similar to those previously observed in Holland, although the actual prevalence detected in this study is likely to be higher than reported because only birds with gross lesions were subjected to culture. Further molecular characterization with a set of six mycobacterial interspersed repetitive unitÁvariable number tandem repeat loci was used to sub-type the isolates in order to show the existence of possible epidemiological links. Six different genotypes were found, which points to infection from multiple foci. No temporal or geographical aggregation of the cases was observed to be associated with the presence of positive birds or with the different variable number tandem repeat allelic profiles. The most feasible origin might be water or food sources, although the reservoir of mycobacteria remains unknown.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Avian mycobacteriosis in free-living raptors in Majorca Island, Spain

Millán

Negre²,

Castellanos

et al. 2010

Avian Pathology

View full text Add to dashboard Cite

show abstract

“…Some PCR systems that target IS900 also can give false-positive results with DNA from mycobacteria other than MAP and with DNA from other types of organisms (Möbius et al, 2008a;2008b). Due to this, new protocols avoiding cross-reactions have been reported (Bull et al, 2003;Herthnek and Bölske, 2006;Kawaji et al, 2007). In response to the uncertainty about the specificity of PCR systems that target IS900 for the identification of MAP, the use of several other target sequences for MAP identification systems have been proposed: ISMap02, ISMav2, hspX, locus 255, and F57 (Stabel and Bannantine, 2005;Slana et al, 2009;Kralik et al, 2010;Sidoti et al, 2011;Keller et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Fecal culture and two fecal-PCR methods for the diagnosis of Mycobacterium avium subsp. paratuberculosis in a seropositive herd

Correa-Valencia

Ramírez

Bülte

et al. 2017

Rev Colomb Cienc Pecu

View full text Add to dashboard Cite

Multiple‐locus variable number tandem repeats analysis for genetic fingerprinting of pathogenic bacteria

2005

View full text Add to dashboard Cite

DNA fingerprinting has attracted considerable interest as means for identifying, tracing and preventing the dissemination of infectious agents. Various methods have been developed for typing of pathogenic bacteria, which differ in discriminative power, reproducibility and ease of interpretation. During recent years a typing method, which uses the information provided by whole genome sequencing of bacterial species, has gained increased attention. Short sequence repeat (SSR) motifs are known to undergo frequent variation in the number of repeated units through cellular mechanisms most commonly active during chromosome replication. A class of SSRs, named variable number of tandem repeats (VNTRs), has proven to be a suitable target for assessing genetic polymorphisms within bacterial species. This review attempts to give an overview of bacterial agents where VNTR-based typing, or multiple-locus variant-repeat analysis (MLVA) has been developed for typing purposes, together with addressing advantages and drawbacks associated with the use of tandem repeated DNA motifs as targets for bacterial typing and identification.

show abstract

Mycobacterial interspersed repetitive units (MIRU) differentiate Mycobacterium avium subspecies paratuberculosis from other species of the Mycobacterium avium complex

Cited by 80 publications

References 27 publications

Avian mycobacteriosis in free-living raptors in Majorca Island, Spain

Avian mycobacteriosis in free-living raptors in Majorca Island, Spain

Fecal culture and two fecal-PCR methods for the diagnosis of Mycobacterium avium subsp. paratuberculosis in a seropositive herd

Multiple‐locus variable number tandem repeats analysis for genetic fingerprinting of pathogenic bacteria

Contact Info

Product

Resources

About