Mycobacterial infection identified with broad‐range <scp>PCR</scp> amplification and suspension array identification

Carlos, Casey A.; Tang, Yi‐Wei; Adler, Donald J.; Kovarik, Carrie L.

doi:10.1111/j.1600-0560.2012.01934.x

Cited by 8 publications

(14 citation statements)

References 8 publications

(18 reference statements)

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“…They have been reported to detect cutaneous mycobacterial infection, and superficial and invasive mycoses (Campa et al, 2008;Carlos, Tang, Adler, & Kovarik, 2012;Sato et al, 2010). Profiling of the microbiota of healing wounds shows an association of Acinetobacter with wound failure, and enteric-associated flora with successful healing .…”

Section: Skin Soft Tissue and Joint Infectionsmentioning

confidence: 98%

Solid and Suspension Microarrays for Microbial Diagnostics

Miller

Karaöz

Brodie

et al. 2015

Methods in Microbiology

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Section: Skin Soft Tissue and Joint Infectionsmentioning

confidence: 98%

Solid and Suspension Microarrays for Microbial Diagnostics

Miller

Karaöz

Brodie

et al. 2015

Methods in Microbiology

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“…12,13 Another case of cutaneous MAC led to the formation of edematous plaque on the left thumb with scales and small erosions. 15 A skin biopsy specimen showed an epidermal hyperkeratosis with an inflammatory infiltrate and granuloma formation. If untreated, the lesions have been shown to persist for weeks to years, but the bacteria do not usually disseminate, and, if treated with the correct antibiotics, symptoms will resolve after several months.…”

Section: Presentationmentioning

confidence: 99%

“…If untreated, the lesions have been shown to persist for weeks to years, but the bacteria do not usually disseminate, and, if treated with the correct antibiotics, symptoms will resolve after several months. 11,12,[14][15][16] According to one dermatology paper, MAC was suspected to be auto-inoculated into an open wound as the patient was a sheep-breeder, and the case was limited to cutaneous spread. 13 One case reported erythematous swelling with fragmentation of the fourth PIP joint caused by MAC diagnosed as an atypical case of destructive monoarthritis, and another case of septic arthritis was found in the left sternoclavicular joint.…”

Section: Presentationmentioning

confidence: 99%

“…Many confirmed cases of immunocompetent patients with MAC infections have reported that the initial culture and/or staining processes yielded negative results. 15,18,19,[21][22][23] Therefore, a differential diagnosis with MAC should not be dismissed simply based on initial staining results, especially if other common disorders are excluded. Negative results may be common due to mycobacterial species special growth condition requirements and a longer incubation period (3-6 weeks).…”

Section: Diagnosismentioning

confidence: 99%

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Extrapulmonary Mycobacterium avium complex infections in immunocompetent patients

Yim¹,

Sotello²,

Nugent³

2020

The Chronicles

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The Mycobacterium avium complex (MAC) includes ubiquitous bacteria that typically cause infection in immunocompromised patients. This paper reviews the presentation, diagnosis, pathogenesis, and treatment of extrapulmonary MAC infections in immunocompetent patients by compiling information from case reports identified by a PubMed search. Mycobacterium avium complex infections in immunocompetent patients can present primarily with extrapulmonary symptoms, and this makes the diagnosis of MAC infection in these patients more difficult. The American Thoracic Society has not established criteria for the diagnosis of extrapulmonary MAC infections; testing for MAC should be based on clinical suspicion or after the exclusion of all other causative agents. Methods of testing should include molecular and biochemical tests, since these tests provide more definitive identification than routine cultures. Extrapulmonary presentations usually respond well to macrolide based multi-drug regimens started as soon as a MAC infection is identified. Key words: Mycobacterium avium, infection, diagnosis, treatment

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“…With a detection limit of 50 to 500 copies/g of tissue, this assay is expected to be more sensitive than AFB staining for the diagnosis of mycobacterial skin infections. This system has recently been reported to promote the diagnosis of an M. avium-M. intracellulare-caused skin infection by detection and identification to the species level of mycobacterial pathogens in FFPE skin biopsy specimens (15).…”

mentioning

confidence: 99%

Mycobacterium smegmatis in Skin Biopsy Specimens from Patients with Suppurative Granulomatous Inflammation

Lü

Xia

et al. 2013

J Clin Microbiol

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Formalin-fixed, paraffin-embedded skin biopsy specimens, including 72 suppurative granulomatous inflammation (SGI) and 47 non-SGI controls, were tested for mycobacteria by using a broad-range PCR and a suspension array identification system. Mycobacterium smegmatis was detected in 13 (18.1%) of the SGI skin biopsy specimens, which was significantly more than 2 (4.3%) in the controls (odds ratio, 5.73; 95% confidence interval, 1.21 to 27.06; P ‫؍‬ 0.028).G ranulomatous inflammation of the skin may be broadly classified as infectious or noninfectious, with the presence of a suppurative inflammatory component often associated with the former (1-3). Suppurative granulomatous inflammation (SGI) has been defined as inflammation within the dermis and subcutis consisting of neutrophils, various numbers of eosinophils, and the presence of a histiocytic population of cells (1, 2). Multinucleated giant cells are usually present without obvious foreign material. A diagnosis may be made histologically; however, prior antibiotic exposure may "sterilize" the tissue, leading to a delayed diagnosis and increased morbidity. Tissue culture is the diagnostic gold standard, but when a mycobacterial infection is responsible, cultures often take weeks to grow (4, 5).To date, about one-third of the species of nontuberculous mycobacteria (NTM) described have been associated with human disease (5). Clinical presentations include infections of the skin and soft tissue, lymph nodes, joints, and lungs. Disseminated infections may arise following the contamination of traumatic or surgical wounds with contaminated water or other materials (5, 6). Most species of NTM have been associated with skin and soft tissue infections, but rapidly growing mycobacteria (Mycobacterium fortuitum, M. chelonae, and M. abscessus), M. marinum, and M. ulcerans have been the ones most frequently described. Some of these organisms exhibit geographic restrictions. The timely and accurate diagnosis of cutaneous NTM infections is important in that different species of these organisms have different antibiotic susceptibilities (4, 6, 7). Current laboratory techniques for identifying mycobacteria to the species level include growth in broth, nucleic acid probing, and sequencing, as well as subculture on solid medium for phenotypic testing, each of which is time intensive (4,7,8).We have developed a MycoID assay that incorporates broadrange PCR amplification with suspension array detection to identify 17 mycobacterial complexes, groups, and species in a single reaction with a detection limit of 50 to 500 copies/g of tissue (8). In this study, we have employed the MycoID procedure to detect and identify mycobacterial species in formalin-fixed, paraffin-embedded (FFPE) skin biopsy specimens exhibiting SGI and non-SGI controls.(This study was presented in part at the Association for Molecular Pathology Annual Meeting, Grapevine, TX, 17 to 19 November 2011.)Study materials. The study FFPE tissue specimens consisted of 119 skin biopsy specimens obtained from Beijing Children...

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Mycobacterial infection identified with broad‐range PCR amplification and suspension array identification

Cited by 8 publications

References 8 publications

Solid and Suspension Microarrays for Microbial Diagnostics

Solid and Suspension Microarrays for Microbial Diagnostics

Extrapulmonary Mycobacterium avium complex infections in immunocompetent patients

Mycobacterium smegmatis in Skin Biopsy Specimens from Patients with Suppurative Granulomatous Inflammation

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