Myco-metabolites as biological control agents against the two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae)

Osman, Mohamed; El-Nasr, Amany Abo; Nawar, Mohamed A.A.; Hefnawy, Gohyza

doi:10.1186/s41938-019-0166-0

Cited by 5 publications

(6 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Beauvericin showed noticeable acaricidal properties, with LD 50 values from 0.66 to 33 µg/mL, depending on the sensitivity of the T. urticae population to the toxin [ 66 ]. The extract of the soil micromycete Aspergillus melleus , in which mellamide, ochratoxin C, nodulisporic acid, 7-oxocurvularin, and 6-(4′-hydroxy-2′-methyl phenoxy)-(−)-(3R)-mellein were detected, was significantly less toxic to the mites, with an LD 50 of 10 mg/mL [ 67 ]. Thus, TeA demonstrated low activity against sucking insects in the contact intestinal assays.…”

Section: Discussionmentioning

confidence: 99%

Entomotoxic Activity of the Extracts from the Fungus, Alternaria tenuissima and Its Major Metabolite, Tenuazonic Acid

Salimova

Dalinova

Dubovik

et al. 2021

JoF

View full text Add to dashboard Cite

The study of fungal antibiotics in their competitive interactions with arthropods may lead to the development of novel biorational insecticides. Extracts of Alternaria tenuissima MFP253011 obtained using various methods showed a wide range of biological activities, including entomotoxic properties. Analysis of their composition and bioactivity allowed us to reveal several known mycotoxins and unidentified compounds that may be involved in the entomotoxic activity of the extracts. Among them, tenuazonic acid (TeA), which was the major component of the A. tenuissima extracts, was found the most likely to have larvicidal activity against Galleria mellonella. In the intrahaemocoel injection bioassay, TeA was toxic to G. mellonella and of Zophobas morio with an LT50 of 6 and 2 days, respectively, at the level of 50 µg/larva. Administered orally, TeA inhibited the growth of G. mellonella larvae and caused mortality of Acheta domesticus adults (LT50 7 days) at a concentration of 250 µg/g of feed. TeA showed weak contact intestinal activity against the two phytophages, Tetranychus urticae and Schizaphis graminum, causing 15% and 27% mortality at a concentration of 1 mg/mL, respectively. TeA was cytotoxic to the Sf9 cell line (IC50 25 µg/mL). Thus, model insects such as G. mellonella could be used for further toxicological characterization of TeA.

show abstract

Section: Discussionmentioning

confidence: 99%

Entomotoxic Activity of the Extracts from the Fungus, Alternaria tenuissima and Its Major Metabolite, Tenuazonic Acid

Salimova

Dalinova

Dubovik

et al. 2021

JoF

View full text Add to dashboard Cite

show abstract

“…has pathogenic effects on the adult stage of D. gallinae, as a bio-control agent. Mohamed et al 2019 showed that, efficacy of four fungal strains Aspergillus melleus, Aspergillus terreus, Emericella nidulans, and Chaetomium globosum against Tetranychus urticae under Laboratory conditions. Also, observed LC50 values were 10.…”

Section: Acaricidal Activity Of a Aflatoxiformans Against T Urticaementioning

confidence: 99%

Isolation and Identification of The Fungal Pathogen Aspergillus aflatoxiformans Strain and Its Role in Controlling The Two-Spotted Spider Mite Tetranychus urticae (Koch) عزل وتعريف المسبب المرضي Aspergillus aflatoxiformans ودوره في مکافحة أکاروس العنکبوت الأحمر ذي البقعتين Tetranychus urticae (Koch)

Emam

Mohamed

2021

Journal of Plant Protection and Pathology

View full text Add to dashboard Cite

The two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae) is the major mite pest infesting many cultivated crops. The fungus, Aspergillus aflatoxiformans Frisvad, Ezekiel, Samson & Houbraken produces aflatoxins where it considered as secondary polyketide metabolites, which cause the death of pests. Therefore, this work aimed to isolate and identify this fungus by amplify the internal transcribed spacer (ITS) region of the rDNA, as well as to evaluate the efficiency of this fungus to control T. urticae. Results showed 300bp DNA product corresponding to the omt-A gene. The existence of omt-A gene in A. aflatoxiformans reflected its ability to produce aflatoxin, which may be responsible of mite control. The efficacy of A. aflatoxiformans against T. urticae recorded the highest reduction percentage of eggs hatch at concentration of 1x10 8 conidia/ml within the 5 th and 6 th day after exposure. These percentages were 99.42 and 100%, respectively. The highest mortality percentage was recorded on the 4 th , 5 th and 6 th day at higher concentration of 1x10 8 . No eggs and larvae of T. urticae were observed within the 5 th and 6 th day after treatment at 1x10 8 . There were not observed nymphs at all tested concentrations. Microelectronic photos showed fungal conidia with cluster shaped which were developed at all T. urticae body surface 6 days after treatments at 1x10 7 and 1x10 8 . Results also confirmed that A. aflatoxiformans could be used successfully to control T. urticae eggs, immature stages and adults. Therefore, this fungus could be involved in any IPM programs of the two-spotted mite.

show abstract

“…Beauvericin showed noticeable acaricidal properties with LD50 from 0.66 to 33 µg/mL, depending on the sensitivity of the T. urticae population to the toxin [66]. The extract of the soil micromycete Aspergillus melleus, in which mellamide, ochratoxin C, nodulisporic acid, 7-oxocurvularin, and 6-(4'-hydroxy-2'-methyl phenoxy)-(−)-(3R)-mellein were detected, was significantly less toxic to the mites with LD50 10 mg/L [67]. Thus, TeA demonstrated low activity against sucking insects in the contact-intestinal assays.…”

Section: Entomotoxicity Of Tenuazonic Acidmentioning

confidence: 99%

Entomotoxic Activity of the Extracts from the Fungus, <em>Alternaria tenuissima</em> and Its Major Metabolite, Tenuazonic Acid

Salimova¹,

Dalinova²,

Dubovik³

et al. 2021

Preprint

View full text Add to dashboard Cite

Study of fungal antibiotics in their competitive interactions with arthropods may lead to development novel biorational insecticides. Extracts of Alternaria tenuissima MFP253011 obtained by various methods showed a wide range of biological activity, including entomotoxic properties. Analysis of their composition and bioactivity allowed to reveal several known mycotoxins and unidentified compounds that may be involved in entomotoxic activity of the extracts. Among them, tenuazonic acid (TeA), which was the major component of the A. tenuissima extracts, was found the most likely to have larvicidal activity against Galleria mellonella. In the intrahaemocoel injection bioassay, TeA was toxic to G. mellonella and of Zophobas morio with LT50 6 and 2 days, respectively, at the level of 50 µg/larva. Administered orally, TeA inhibited growth of G. mellonella larvae and caused mortality of Acheta domesticus imagines (LT50 7 days) at a concentration of 250 µg/g of feed. TeA showed weak contact-intestinal activity against the two phytophages, Tetranychus urticae and Schizaphis graminum, causing the 12 and 40% of mortality at a concentration of 1 mg/mL. TeA was cytotoxic to Sf9 cell line (IC50 25 µg/mL). Thus, model insect G. mellonella and cell line Sf9 could be used for a further toxicological characterization of TeA.

show abstract

Myco-metabolites as biological control agents against the two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae)

Cited by 5 publications

References 15 publications

Entomotoxic Activity of the Extracts from the Fungus, Alternaria tenuissima and Its Major Metabolite, Tenuazonic Acid

Entomotoxic Activity of the Extracts from the Fungus, Alternaria tenuissima and Its Major Metabolite, Tenuazonic Acid

Entomotoxic Activity of the Extracts from the Fungus, <em>Alternaria tenuissima</em> and Its Major Metabolite, Tenuazonic Acid

Contact Info

Product

Resources

About