Mycetocola spongiae sp. nov., isolated from deep-sea sponge Cacospongia mycofijiensis

Chen, Yuling; Jin, Shan; Sun, Wei; Song, Qianqian; Li, Zhiyong

doi:10.1099/ijsem.0.005291

“…Acetate buffer was used for pH 4.0–5.5, phosphate buffer was used for pH 6.0–8.0 and Tris buffer was used for pH 8.5–10.0. According to the manufacturer’s instructions; anaerobic growth was determined on an MA medium at 20 °C after 3 days of incubation using AnaeroPack-Anaero (MGC) [33]. Cell morphology was observed by a scanning electron microscope (Sirion 200, FEI) after 3 days of incubation in MB medium at 20 °C.…”

Section: Full-textmentioning

confidence: 99%

Brevibacterium spongiae sp. nov., isolated from marine sponge Hymeniacidon sp.

Zhang

¹

,

Song

²

,

Jin

³

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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A novel bacterial strain, designated as WHS-Z9T, was isolated from marine sponge Hymeniacidon sp. collected from Weihai (37° 25′ N, 121° 58′ E), Shandong Province, PR China. Cells of strain WHS-Z9T were Gram-stain-positive, non-spore-forming, non-motile, short-rod-shaped and light yellow-pigmented. The strain could grow at 10–40 °C (optimum, 20 °C), pH 4.5–9.5 (optimum, pH 8.5) and 2–14 % (w/v) NaCl (optimum, 4 %). The 16S rRNA gene sequence of strain WHS-Z9T showed 98.7 % similarity to that of Brevibacterium epidermidis NBRC 14811T, 98.5 % to Brevibacterium sediminis FXJ8.269T and 98.4 % to Brevibacterium oceani BBH7T. The phylogenetic tree based on 16S rRNA gene sequences revealed that strain WHS-Z9T was clustered with Brevibacterium limosum o2T. The whole genome of WHS-Z9T was approximately 4 217 721 bp in size with a G+C content of 65.2 %. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values among WHS-Z9T and other Brevibacterium type strains were 83.3–85.5 % (ANI based on blast), 86.4–87.9 % (ANI based on MUMmer) and 41.9–57.5 % (dDDH). Percentage of conserved protein values between the genomes of strain WHS-Z9T and members of genera Brevibacterium were 76.8–82.9 %, while the average amino acid identity (AAI) values were 83.7–87.0 %. The dDDH, ANI, AAI and POCP values were below the standard cut-off criteria for the delineation of bacterial species. The sole respiratory quinone in strain WHS-Z9T was MK-8(H2), and the predominant fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0. The major polar lipids of WHS-Z9T consisted of diphosphatidylglycerol and glycolipid. The diagnostic cell-wall diamino acid of strain WHS-Z9T was meso-diaminopimelic acid. Based on the data obtained in this study, strain WHS-Z9T (=MCCC 1K07845T=KCTC 49848T) should be classified as the type strain of a novel species of the genus Brevibacterium , for which the name Brevibacterium spongiae sp. nov. is proposed.

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“…Sponges are sources of novel microbes [14]. In this study, a strain, designated CY-G T , was isolated from a sponge collected from the Red Sea (29° 31′ 28.1″ N, 34° 56′ 05.5″ E) in December 2020.…”

Section: Full-textmentioning

confidence: 99%

“…Species of the genus Cytobacillus have been isolated from plants [1, 2], soil [3][4][5][6][7], the intestinal tract of an earthworm [8], water [9, 10], a pharmaceutical manufacturing site [11], a spacecraft-assembly clean room [12] and food [13] (Table 1). Sponges are sources of novel microbes [14]. In this study, a strain, designated CY-G T , was isolated from a sponge collected from the Red Sea (29° 31′ 28.1″ N, 34° 56′ 05.5″ E) in December 2020.…”

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confidence: 99%

Cytobacillus spongiae sp. nov. isolated from sponge Diacarnus spinipoculum

Gao

¹

,

Song

²

,

Jin

³

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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A novel Gram-stain-positive, aerobic and motile bacterium, designated strain CY-GT, was isolated from a sponge (Diacarnus spinipoculum) collected from the Red Sea. The strain grew at 13–43 °C (optimum 30 °C), pH 5.5–10.0 (optimum pH 9.0) and with 0–8.0 % (w/v) (0–1.37 M) NaCl (optimum 0 %). The results of phylogenetic analysis based on the 16S rRNA gene sequences indicated that CY-GT represents a member of the genus Cytobacillus , with the highest sequence identity to Cytobacillus oceanisediminis H2T (97.05 %), followed by Cytobacillus firmus IAM 12464T (96.76 %). The major cellular fatty acids (>5 % of the total) of CY-GT were C15 : 0iso, C16 : 0iso, C16 : 1ω7c alcohol, C16 : 0, C17 : 1iso ω10c and C17 : 0iso. The major polar lipids were glycolipid, diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The major respiratory quinone is menaquinone-7 (MK-7). The cell-wall peptidoglycan contains meso-diaminopimelic acid. The total genome size of CY-GT is 4 789 051 bp. The DNA G+C content is 38.83 mol%. The average nucleotide identity and DNA–DNA hybridization among CY-GT and type strains of other species of the genus Cytobacillus were 76.79–78.97 % and 20.10–24.90 %, respectively. On the basis of the results of phylogenetic analysis, physiological and biochemical characterization, strain CY-GT represents a novel species of the genus Cytobacillus , for which the name Cytobacillus spongiae sp. nov. is proposed. The type strain is CY-GT (=MCCC 1K06383T=KCTC 43348T).

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“…Growth at different salt concentrations and pH levels were determined in MB after incubation for 7 days by measuring the OD 600 (Multifunctional enzyme marker-Spark; Tecan). According to the manufacturer’s instructions, anaerobic growth was determined in MA medium at 30 ℃ after 7 days of incubation using the AnaeroPack-Anaero (MGC) [38]. The cells were grown in MB at 30 ℃ after 7 days of incubation, and cell morphology was observed with a scanning electron microscope (Sirion 200, FEI).…”

Section: Full-textmentioning

confidence: 99%

Qipengyuania spongiae sp. nov., isolated from marine sponge Cinachyrella kuekenthali

Zhang

¹

,

Song

²

,

Jin

³

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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A novel bacterial strain, designated as PHS-Z21T, was isolated from the marine sponge Cinachyrella kuekenthali collected from PG Dave’s Rock, Philippines. Cells of PHS-Z21T are Gram-stain-negative, non-motile, pale-yellow-pigmented, short rods. PHS-Z21T is able to grow at 10–40 ℃ (optimum, 30 ℃), pH 5.5–9.0 (optimum, pH 8.5) and with 3–9 % (w/v) NaCl (optimum, 4 %). Its 16S rRNA gene sequence shows 98.6 % similarity to Qipengyuania nanhaisediminis CGMCC 1.7715T, 98.5 % similarity to Qipengyuania vulgaris 022-2-10T and 98.4 % similarity to Qipengyuania flava SW-46T, respectively. The phylogenetic tree based on 16S rRNA gene sequences reveals that PHS-Z21T is clustered with Q. flava SW-46T. The total genome of PHS-Z21T is approximately 2 932 896 bp in size with a DNA G+C content of 64.7 %. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values among PHS-Z21T and other type strains are 70.0–77.3 % (ANIb), 83.3–86.8 % (ANIm) and 13.0–26.9 % (dDDH), respectively. The dDDH and ANI values are below the standard cutoff criteria for delineating bacterial species. Percentage of conserved proteins (POCP) values between the genome of strain PHS-Z21T and those of members of the genera Qipengyuania , Erythrobacter , Altererythrobacter and Alteriqipengyuania were 62.0–74.5 %, 55.8–63.2 %, 60.7–66.9 % and 63.9–66.8%, respectively, while the AAI values were 68.4–74.3 %, 63.8–65.9 %, 66.3–68.3 % and 64.7–66.9%, respectively. The major fatty acids of PHS-Z21T are composed of summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), C18 : 1ω7c 11-methyl, C16 : 0 and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c). The polar lipids of PHS-Z21T mainly consist of diphosphatidylglycerol, glycolipid, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and glycophospholipid. The respiratory lipoquinone was identified as Q-10. On the basis of the phenotypic and phylogenetic data, strain PHS-Z21T represents a novel species of the genus Qipengyuania , for which the name Qipengyuania spongiae sp. nov. is proposed. The type strain is PHS-Z21T (=MCCC 1K07849T=KCTC 92590T).

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Mycetocola spongiae sp. nov., isolated from deep-sea sponge Cacospongia mycofijiensis

Cited by 10 publications

References 23 publications

Brevibacterium spongiae sp. nov., isolated from marine sponge Hymeniacidon sp.

Brevibacterium spongiae sp. nov., isolated from marine sponge Hymeniacidon sp.

Cytobacillus spongiae sp. nov. isolated from sponge Diacarnus spinipoculum

Qipengyuania spongiae sp. nov., isolated from marine sponge Cinachyrella kuekenthali

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