Mycelium polysaccharides of <i>Macrolepiota procera</i> alleviate reproductive impairments induced by nonylphenol

Wang, Wenshuai; Li, Xiaoxu; Zhang, Yaohan; Zhang, Jianjun; Jia, Le

doi:10.1039/d2fo00680d

Cited by 8 publications

(4 citation statements)

References 58 publications

(76 reference statements)

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“…These complexes combine the beneficial effects of both PSs and proteins. Furthermore, it has been found that MP mycelium PSs show protective activity on experimental male mice against nonylphenol toxicity via alleviating the oxidative stress, apoptosis, autophagy, and inflammatory responses in testicular cells [ 35 ]. Water-extractable PSs from the wild parasol mushroom have exhibited antioxidant and prebiotic activities in vitro, but their structure and immunomodulatory effects have not been elucidated [ 36 , 37 ].…”

Section: Introductionmentioning

confidence: 99%

Structural Features and Immunomodulatory Effects of Water-Extractable Polysaccharides from Macrolepiota procera (Scop.) Singer

Georgiev

Vašíček

Dzhambazov

et al. 2022

JoF

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Macrolepiota procera (MP) is an edible mushroom used in the treatment of diabetes, hypertension and inflammation. However, the structure and biological effects of its polysaccharides (PSs) are unclear. This study investigates the structural features of a PS complex from MP (MP-PSC), its immunomodulatory activities and effects on probiotic and pathogenic bacteria. MP-PSC was obtained by boiling water, and PSs were characterized by 2D NMR spectroscopy. The immunomodulatory effects on blood and derived neutrophils, other leukocytes, and murine macrophages were studied by flow cytometry, chemiluminescence, spectrophotometry, and ELISA. The total carbohydrate content of MP-PSC was 74.2%, with glycogen occupying 36.7%, followed by β-D-glucan, α-L-fuco-2-(1,6)-D-galactan, and β-D-glucomannan. MP-PSC (200 μg/mL) increased the number of CD14+ monocyte cells in the blood, after ex vivo incubation for 24 h. It dose-dependently (50–200 μg/mL) activated the spontaneous oxidative burst of whole blood phagocytes, NO, and interleukin 6 productions in RAW264.7 cells. MP-PSC exhibited a low antioxidant activity and failed to suppress the oxidative burst and NO generation, induced by inflammatory agents. It (2.0%, w/v) stimulated probiotic co-cultures and hindered the growth and biofilm development of Escherichia coli, Streptococcus mutans and Salmonella enterica. MP PSs can be included in synbiotics to test their immunostimulating effects on compromised immune systems and gut health.

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Section: Introductionmentioning

confidence: 99%

Structural Features and Immunomodulatory Effects of Water-Extractable Polysaccharides from Macrolepiota procera (Scop.) Singer

Georgiev

Vašíček

Dzhambazov

et al. 2022

JoF

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“…The approximate MW of the polysaccharide extract from M. procera (from China) obtained by the ethanol precipitation from hot water extract method was expressed as 7.71 × 10 5 Da . Qu et al calculated the approximate MW of Hygrophorus lucorum polysaccharide extract (obtained by the ethanol precipitation from the hot water extract method) as 20.4 kDa .…”

Section: Resultsmentioning

confidence: 99%

“… 23 The approximate MW of the polysaccharide extract from M. procera (from China) obtained by the ethanol precipitation from hot water extract method was expressed as 7.71 × 10 5 Da. 38 Qu et al calculated the approximate MW of Hygrophorus lucorum polysaccharide extract (obtained by the ethanol precipitation from the hot water extract method) as 20.4 kDa. 39 The main reason for this discrepancy in the approximate MWs of polysaccharide extracts between the results in the literature and our research may be due to the differences in mushroom species and extraction methods or applications.…”

Section: Resultsmentioning

confidence: 99%

Characterization of Polysaccharide Extracts of Four Edible Mushrooms and Determination of In Vitro Antioxidant, Enzyme Inhibition and Anticancer Activities

Deveci,

Tel-Çayan,

Çayan

et al. 2024

ACS Omega

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Mushroom polysaccharides are important bioactive compounds derived from mushrooms with various beneficial properties. In this study, the chemical characterization and bioactivities of polysaccharide extracts from four different edible mushrooms, Clavariadelphus truncatus Donk, Craterellus tubaeformis (Fr.) Queĺ., Hygrophorus pudorinus (Fr.) Fr., and Macrolepiota procera (Scop.) Singer were studied. Glucose (13.24−56.02%), galactose (14.18−64.05%), mannose (2.18−18.13%), fucose (1.21−5.78%), and arabinose (0.04−5.43%) were identified in all polysaccharide extracts by GC-MS (gas chromatography−mass spectrometry). FT-IR (Fourier transform infrared spectroscopy) confirmed the presence of characteristic carbohydrate patterns. 1 H NMR suggested that all polysaccharide extracts had αand β-Dmannopyranose, D-glucopyranose, D-galactopyranose, α-L-arabinofuranose, and α-L-fucopyranose residues. Approximate molecular weights of polysaccharide extracts were determined by HPLC (high-performance liquid chromatography). The best antioxidant activity was found in M. procera polysaccharide extract in DPPH • (1,1-diphenyl-2-picrylhydrazyl) scavenging (39.03% at 800 μg/ mL), CUPRAC (cupric reducing antioxidant capacity) (A 0.50 : 387.50 μg/mL), and PRAP (phosphomolybdenum reducing antioxidant power) (A 0.50 : 384.08 μg/mL) assays. C. truncatus polysaccharide extract showed the highest antioxidant activity in ABTS •+ scavenging (IC 50 : 734.09 μg/mL), β-carotene-linoleic acid (IC 50 : 472.16 μg/mL), and iron chelating (IC 50 : 180.35 μg/mL) assays. Significant anticancer activity was found in C. truncatus polysaccharide extract on HT-29 (IC 50 : 46.49 μg/mL) and HepG2 (IC 50 : 48.50 μg/mL) cell lines and H. pudorinus polysaccharide extract on the HeLa cell line (IC 50 : 51.64 μg/mL). Also, H. pudorinus polysaccharide extract possessed prominent AChE (acetylcholinesterase) inhibition activity (49.14% at 200 μg/mL).

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“…At least 50 seminiferous tubules were analyzed in each mouse in order to obtain an effective, accurate, and representative evaluation. 25,29…”

Section: Histopathological Analysesmentioning

confidence: 99%

Cyanidin-3-O-glucoside and protocatechuic acid alleviate heat stress-induced testicular damage

Cai

Liu

et al. 2023

Food Funct.

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Mycelium polysaccharides of Macrolepiota procera alleviate reproductive impairments induced by nonylphenol

Abstract: Mycelium polysaccharides of Macrolepiota procera (MMP) had positive role in amelioration of NP-induced reproductive disorders.

Cited by 8 publications

References 58 publications

Structural Features and Immunomodulatory Effects of Water-Extractable Polysaccharides from Macrolepiota procera (Scop.) Singer

Structural Features and Immunomodulatory Effects of Water-Extractable Polysaccharides from Macrolepiota procera (Scop.) Singer

Characterization of Polysaccharide Extracts of Four Edible Mushrooms and Determination of In Vitro Antioxidant, Enzyme Inhibition and Anticancer Activities

Cyanidin-3-O-glucoside and protocatechuic acid alleviate heat stress-induced testicular damage

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