Mycelium Development and Architecture, and Spore Production of Scutellospora reticulata in Monoxenic Culture with Ri T-DNA Transformed Carrot Roots

Souza, F. A. de; Declerck, Stéphane

doi:10.2307/3761908

Cited by 29 publications

(34 citation statements)

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“…We can hypothesize that the mycorrhizal community occurring at Forest sites are composed of more fastidious species that would take a longer time to colonize the root cortex and to reach a threshold level of colonization before sporulating (6). Therefore, we can also invoke that AMF species has distinct r and k strategies (34,36). High sporulation in trap culture might reflect an r strategy of some species that allocate most of the carbon to sporulation while a k strategy might be represented by those species that are not prolific sporulators and allocate resource to vegetative growth.…”

Section: Discussionmentioning

confidence: 99%

Occurrence and diversity of arbuscular mycorrhizal fungi in trap cultures from soils under different land use systems in the Amazon, Brazil

Leal¹,

Stürmer²,

Siqueira³

2009

Braz. J. Microbiol.

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The aim of this work was to evaluate the occurrence of arbuscular mycorrhizal fungi (AMF) species diversity in soil samples from the Amazon region under distinct land use systems (Forest, Old Secondary Forest, Young Secondary Forest, Agroforestry systems, Crops and Pasture) using two distinct trap cultures. Traps established using Sorghum sudanense and Vigna unguiculata (at Universidade Regional de Blumenau -FURB) and Brachiaria decumbens and Neonotonia wightii (at Universidade Federal de Lavras -UFLA) were grown for 150 days in greenhouse conditions, when spore density and species identification were evaluated. A great variation on species richness was detected in several samples, regardless of the land use systems from where samples were obtained. A total number of 24 AMF species were recovered using both methods of trap cultures, with FURB´s traps yielding higher number of species. Acaulospora delicata, A. foveata, Entrophospora colombiana and two undescribed Glomus species were the most abundant and frequent species recovered from the traps. Number of species decreased in each genus according to this order: Acaulospora, Glomus, Entrophospora, Gigaspora, Archaeospora, Scutellospora and Paraglomus. Spore numbers were higher in Young Secondary Forest and Pastures. Our study demonstrated that AMF have a widespread occurrence in all land use systems in Amazon and they sporulate more abundantly in trap cultures from land uses under interference than in the pristine Forest ecosystem.

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Section: Discussionmentioning

confidence: 99%

Occurrence and diversity of arbuscular mycorrhizal fungi in trap cultures from soils under different land use systems in the Amazon, Brazil

Leal¹,

Stürmer²,

Siqueira³

2009

Braz. J. Microbiol.

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“…(1998) for the Glomus strains, de Souza & Declerck (2003) for S. reticulata and Bécard & Fortin (1988) for the Gigaspora strains.…”

Section: Biological Materialsmentioning

confidence: 99%

Arbuscular mycorrhizal fungi reveal distinct patterns of anastomosis formation and hyphal healing mechanisms between different phylogenic groups

et al. 2004

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Summary• The significance of anastomosis formation and the hyphal healing mechanism (HHM) for functionality and integrity of the arbuscular mycorrhizal (AM) fungal mycelial network remains poorly documented.• Four Glomeraceae and three Gigasporaceae were cultured monoxenically. Anastomosis formation was assessed using the grid line method, while HHM was time-lapse monitored.• In intact mycelial networks, the number of anastomosis per hyphal length was higher for Glomeraceae than for Gigasporaceae strains. Glomeraceae strains studied always formed anastomosis between different hyphae, whereas anastomosis in the Gigasporaceae more often concerned hyphal bridges within the same hyphae. In both families the HHM corresponded to a four-step process; first septum formation; second initiation of growing hyphal tips (GHTs); third GHT elongation, orientation and contact; and fourth GHT fusion and cytoplasmic/protoplasmic flux re-establishment. These four steps differentiated Glomeraceae from Gigasporaceae .• The type and number of anastomosis per hyphal length, and the HHM differed considerably between Glomeraceae and Gigasporaceae families representing a supplementary character that distinguishes these two families and may be of significance in ecological studies of AM fungi.

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“…Several different methods were employed to propagate AMF such as single or monosporic (Fracchia et al, 2001;Selvakumar et al, 2016), hairy root (de Souza and Declerck 2003), solid substrate (Millner and Kitt 1992;Douds et al, 2010), aeroponic (Mohammad et al, 2000), and hydroponic (Tajini et al, 2009). However, storing the propagated AMF spore requires technical skills and preliminary knowledge, thus making it difficult for farmers.…”

Section: Effect Of Mycorrhizal Inoculation On the Growth Parametersmentioning

confidence: 99%

Trap Culture Technique for Propagation of Arbuscular Mycorrhizal Fungi using Different Host Plants

Selvakumar¹,

Kim²,

Walitang³

et al. 2016

Korean Journal of Soil Science and Fertilizer

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Arbuscular mycorrhizal fungi (AMF) spore propagation and long term maintenance is still a complicated technique for farmers. The use of AMF for their ability to promote plant growth and protect plants against pathogen attack and environmental stresses demands AMF propagation for large scale application. This study aimed to propagate AMF spores by trap culture technique and assess their ability to propagate with different host plants in a continuous plant cycle. Mycorrhizal inoculation by trap culture in maize resulted in longer shoots and roots than sudangrass plants. Increase in dry weight with higher percentage also was observed for maize plants. After first and second plant cycle, maize plants had the higher percentage of mycorrhizal response in terms of colonization and arbuscules than sudangrass. Maximum in spore count also achieved in the pots of maize plants. The results show that maize plant is more suitable host plant for AMF spore propagation and trap culture technique can be used effectively to maintain the AMF culture for long time.

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Mycelium Development and Architecture, and Spore Production of Scutellospora reticulata in Monoxenic Culture with Ri T-DNA Transformed Carrot Roots

Cited by 29 publications

References 0 publications

Occurrence and diversity of arbuscular mycorrhizal fungi in trap cultures from soils under different land use systems in the Amazon, Brazil

Occurrence and diversity of arbuscular mycorrhizal fungi in trap cultures from soils under different land use systems in the Amazon, Brazil

Arbuscular mycorrhizal fungi reveal distinct patterns of anastomosis formation and hyphal healing mechanisms between different phylogenic groups

Trap Culture Technique for Propagation of Arbuscular Mycorrhizal Fungi using Different Host Plants

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