Mycelium Binding and Depolymerization of Synthetic<sup>14</sup>C-Labeled Lignin During Decomposition by<i>Phanerochaete chrysosporium</i>

Chua, Miranda G. S.; Choi, Suki; Kirk, T. Kent

doi:10.1515/hfsg.1983.37.2.55

Cited by 50 publications

(25 citation statements)

References 20 publications

(16 reference statements)

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“…The fixation of lignin to the mycelium has also been reported in several studies (Janshekar et al 1982;Chua et al 1983). This fixation only takes place in ligninolyric cultures, suggesting a direct correlation between the binding of lignin to the mycelium and the lignin degradation (Chua et al 1983).…”

Section: Chrysogporiumsupporting

confidence: 60%

“…This fixation only takes place in ligninolyric cultures, suggesting a direct correlation between the binding of lignin to the mycelium and the lignin degradation (Chua et al 1983). Thus the saturation of the ligninolytic system by lignin observed in our experiments could reflect the maximum amounts of lignin which can bind to the mycelium in a given situation.…”

Section: Chrysogporiummentioning

confidence: 56%

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Influence of lignin peroxidase concentration and localisation in lignin biodegradation by Phanerochaete chrysosporium

Kurek¹,

Odier²

1990

Appl Microbiol Biotechnol

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The lignin mineralization rate in cultures of Phanerochaete chrysosporium increases with lignin peroxidase concentration up to 20 nkat ml -~. At higher concentrations the rate of lignin mineralization decreases with increasing lignin peroxidase concentration. The amount of mycelium is not a limiting factor for lignin mineralization at high exocellular lignin peroxidase concentrations. Cultures that contain lignin peroxidase in association with the mycelium as pellets and no free exocellular enzyme induce a lignin mineralization rate equivalent to cultures reconstituted with washed pellets supplemente d with 15 nkat m l -~ of exogenous free enzyme. These results show that although lignin degradation by lignin peroxidase seems to be facilitated when lignin peroxidase is localised on the surface of the mycelium, free exocellular lignin peroxidase can also efficiently enhance mineralization of lignin by P. chrysosporium.

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Section: Chrysogporiumsupporting

confidence: 60%

Section: Chrysogporiummentioning

confidence: 56%

Influence of lignin peroxidase concentration and localisation in lignin biodegradation by Phanerochaete chrysosporium

Kurek¹,

Odier²

1990

Appl Microbiol Biotechnol

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“…8 Free aromatic acids have been identified among low molecular-weight compounds extracted from wood treated with P. chrysosporium, 17 and the presence of residues of aromatic acids has been suggested by 13 C NMR studies of milled-wood lignin from decayed wood. 18 The results presented here provide additional qualitative and quantitative evidence for the presence of ether-linked residues of aromatic acids and aldehydes. These are formed in situ during fungal attack on guaiacyl (G) and syringyl (S) lignin units, and detected by Py-GC/MS after methylation of the partially-degraded residual lignin.…”

Section: Discussionmentioning

confidence: 57%

Demonstration ofIn Situ Oxidative Degradation of Lignin Side Chains by Two White-rot Fungi Using Analytical Pyrolysis of Methylated Wheat Straw

Camarero

Galletti

Martı́nez

1997

Rapid Commun. Mass Spectrom.

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“…of chloroform (Nakatsubo et al, 1982). Radioactivity in the aqueous and organic layers was determined (Chua et al, 1983), and the aqueous fraction was discarded. Principal products from the model compounds had been identified previously; they and residual compounds (I) and (II) were isolated from the organic fraction by t.l.c.…”

Section: Degradation Of Model Compounds (I) and (Ii) By Intact Culturesmentioning

confidence: 99%

Free hydroxyl radical is not involved in an important reaction of lignin degradation by Phanerochaete chrysosporium Burds

Kirk¹,

Mozuch²

1985

Biochemical Journal

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Hydroxyl radical (HO.) has been implicated in the degradation of lignin by Phanerochaete chrysosporium. This study assessed the possible involvement of HO. in degradation of lignin substructural models by intact cultures and by an extracellular ligninase isolated from the cultures. Two non-phenolic lignin model compounds [aryl-C(alpha)HOH-C(beta)HR-C(gamma)H2OH, in which R = aryl (beta-1) or R = O-aryl (beta-O-4)] were degraded by cultures, by the purified ligninase, and by Fenton's reagent (H2O2 + Fe2+), which generates HO. The ligninase and the cultures formed similar products, derived via an initial cleavage between C(alpha) and C(beta) (known to be an important biodegradative reaction), indicating that the ligninase is responsible for model degradation in cultures. Products from the Fenton degradation were mainly polar phenolics that exhibited little similarity to those from the biological systems. Mass-spectral analysis, however, revealed traces of the same products in the Fenton reaction as seen in the biological reactions; even so, an 18O2-incorporation study showed that the mechanism of formation differed. E.s.r. spectroscopy with a spin-trapping agent readily detected HO. in the Fenton system, but indicated that no HO. is formed during ligninase catalysis. We conclude, therefore that HO. is not involved in fungal C(alpha)-C(beta) cleavage in the beta-1 and beta-O-4 models and, by extension, in the same reaction in lignin.

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Mycelium Binding and Depolymerization of Synthetic¹⁴C-Labeled Lignin During Decomposition byPhanerochaete chrysosporium

Cited by 50 publications

References 20 publications

Influence of lignin peroxidase concentration and localisation in lignin biodegradation by Phanerochaete chrysosporium

Influence of lignin peroxidase concentration and localisation in lignin biodegradation by Phanerochaete chrysosporium

Demonstration ofIn Situ Oxidative Degradation of Lignin Side Chains by Two White-rot Fungi Using Analytical Pyrolysis of Methylated Wheat Straw

Free hydroxyl radical is not involved in an important reaction of lignin degradation by Phanerochaete chrysosporium Burds

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Mycelium Binding and Depolymerization of Synthetic14C-Labeled Lignin During Decomposition byPhanerochaete chrysosporium

Cited by 50 publications

References 20 publications

Influence of lignin peroxidase concentration and localisation in lignin biodegradation by Phanerochaete chrysosporium

Influence of lignin peroxidase concentration and localisation in lignin biodegradation by Phanerochaete chrysosporium

Demonstration ofIn Situ Oxidative Degradation of Lignin Side Chains by Two White-rot Fungi Using Analytical Pyrolysis of Methylated Wheat Straw

Free hydroxyl radical is not involved in an important reaction of lignin degradation by Phanerochaete chrysosporium Burds

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Mycelium Binding and Depolymerization of Synthetic¹⁴C-Labeled Lignin During Decomposition byPhanerochaete chrysosporium