Mycelial Growth of <i>Paecilomyces hepiali</i> in Various Agar Media and Yield of Fruit Bodies in Rice Based Media

Abstract

Growth of Paecilomyces hepiali in various agar media and yield of fruit bodies in rice based media were studied. The best growth in agar media was obtained at 25˚C (61.86 mm colony diameter in 14 days). The initial agar media pH range from 6 to 8 was found to be the most favourable for mycelial growth. This study found that agars made with powders of cereal grains alone do not support good mycelial growth of P. hepiali. Addition of peptone improved mycelial growth significantly. The most favourable carbon sources were Mannose, Fructose and Glucose. Organic nitrogen sources were found to be the most preferred. The results demonstrated that brown rice is better than polished rice in yield of fruit bodies. Addition of peptone was found to be quite significant in enhancing yield of fruit bodies. Peptone, as a supplement, gave a better yield than addition of egg yolk, albumen and a mixture of the two. The medium with 40 g brown rice, 0.325 g glucose, 0.65 g sucrose, 2 g peptone and 65 ml corn steep liquor was found to be the most favourable and it yielded 19.3 g of fresh fruit bodies.

Bioactive Betulin produced by marine Paecilomyces WE3-F

“…Using response surface methodology to optimize the extract process of mycelial polysaccharides from P. hepiali, as well, test antioxidant activities were studied by Yu et al (2011). In addition, the best growth for P. hepiali in an optimized media was obtained at 25°C in 14 days with initial pH range from 6 to 8 (Chioza, and Ohga, 2013). Giraldo et al (2012) observed that P. variotii is able to produce extracellular invertases when grown for 96 h, where, the optimal temperature and pH values were 60°C and 4·0-4·5, respectively.…”

“…Another ten different culture media were used to investigate a mycelia growth of P. fumosoroseus, where, only two media were the favorable carbon and nitrogen source for maximal growth (Shim et al, 2003). Recently, the growth of P. hepiali in various agar media was studied, where, the addition of peptone improved mycelial growth and the most favorable carbon sources were mannose, fructose and glucose (Chioza, and Ohga, 2013).…”

Effects of Hypobaric and Hyperbaric Pressures on Mycelial Growth of Isolated Strain of Wild <i>Ophiocordyceps sinensis</i>

“…Similarly, some chemical compounds might be removed from media by hypobaric pressure and enhances anabolism of P. hepiali mycelia. Artificial mycelial production is one of gaining industry but their researches have mainly been focused on nutrient media composition and typical physical environmental condition [20]. This study has filled that gap and improved the efficiency of mycelial production and it can be implemented for commercial process.…”

“…To determine the most favorable pressure for mycelial growth of P. hepiali on semi solid media; 5 mm diameter agar plugs with actively growing mycelium were inoculated on PDA plates by placing a one plug on the centre of the PDA surface [20]. General procedure was used to prepare 9 cm diameter PDA petri dishes.…”

Vegetative Development of <i>Sparassis crispa</i> in Various Growth Conditions and Effect of Electric Pulse Simulation on Its Fruit Body Production

“…Four inoculated plates were incubated at each of the following temperatures: 15˚C, 20˚C, 25˚C, 30˚C and 35˚C. The measurements of radial mycelial growth were done using the method as described by Chioza and Ohga [12]. The diameter of each petri dish was determined by calculating the average of three diameter measurements.…”