Mycelial Growth and Amylase Production by Talaromyces emersonii

Oso, B. A.

doi:10.2307/3759061

Cited by 5 publications

(2 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…genetical) rather than being imposed. This was also reported by Oso (1979); Padmaja and Balagopal (1985); and Ajiboye (2004). These pathogens also grew well at different proportions on different carbon media which indicates preferential utilization of carbon sources used.…”

Section: Discussionsupporting

confidence: 79%

Post-harvest enzymatic activities of healthy and infected Cassava (Manihot esculenta Crantz) tubers

Salami

Akintokun

2008

Emir. J. Food Agric

View full text Add to dashboard Cite

Post-harvest rot of tubers of three cassava (Manihot esculenta Crantz) cultivars namely TMS 4(2)1425 (hybrid cultivar); and 2 local cultivars Oko-Iyawo and Odongbo) were surveyed in Southwestern Nigeria. A total of ten fungal organisms were isolated from rotted cassava tubers collected from eight towns in four states in Southwestern Nigeria. The most frequently isolated and pathogenic ones which were used in this study as the test pathogens are Lasiodiplodia theobromae; Macrophomina phaseolina; Rhizopus stolonifer; and Fusarium pallidoroseum,. Their cell wall degrading enzymes which are cellulase; amylase; polygalacturonase (PG); and pectin methyl esterase (PME) were also assayed. In all the three cultivars inoculated with each of the test pathogens, enzyme activities were found to increase with the incubation period between 6 and 8 days of inoculation and declined at day 10. The peak of enzyme activities declined at different periods for all the cultivars used. PME and PG activities were found highest in cultivar TMS 4(2) 1425 and least in Odongbo cultivar.Test pathogens also behaved differently when inoculated with the culture filtrates of the different enzyme activities. M. Phaseolina was found highest while F. pallidoroseum was the least in all the enzyme activities cultured except in pectin methyl esterase where Lasiodiplodia theobromae had the highest activity. Mycelia dry weight of the test pathogens were also found to increase with incubation period with F. pallidoroseum having the highest mycelia mats while L. theobromae had the least. Enzyme production and activities of the test pathogens in the utilization of the carbon sources varied with the different carbon sources and the test pathogens. All the test pathogens produced enzymes and had activity but at different levels.Generally, enzyme activities showed the rate of maceration of tissues and the extent of rot disease of cassava tubers as well as the aggressiveness and pathogenic abilities of the test pathogens.

show abstract

Section: Discussionsupporting

confidence: 79%

Post-harvest enzymatic activities of healthy and infected Cassava (Manihot esculenta Crantz) tubers

Salami

Akintokun

2008

Emir. J. Food Agric

View full text Add to dashboard Cite

show abstract

“…Adams and Deploey (1986) obtained high activity of α-amylase from A. flavus with starch as the substrate in the culture medium. Likewise Oso (1979) showed that α-amylase activity could be induced by using soluble starch as the sole substrate. Attia and Ali (1974) also demonstrated that starch, maltose, dextrin and glucose were effective inducers for A. awamori to secrete α-amylase.…”

Section: Sbti and Wbti Inhibit α-Amylase Activity And Aflatoxin B 1 Pmentioning

confidence: 98%

Inhibition of endogenous α-amylase and protease of Aspergillus flavus by trypsin inhibitor from cultivated and wild-type soybean

Zhang

Wang

et al. 2010

Ann Microbiol

View full text Add to dashboard Cite

The anti-Aspergillus flavus activity of trypsin inhibitor (TI) from cultivated and wild-type soybean (SBTI and WBTI) was investigated in order to confirm its ability to reduce the activity of endogenous α-amylase, protease enzymes and production of aflatoxin B 1 secreted by A. flavus. In the current study, it was demonstrated that purified SBTI/WBTI belonged to the family of Bowman-Birk TI, based on evidence from amino acid composition, the presence of two independent binding sites for trypsin and chymotrypsin, and a lysine residue as the active site for trypsin inhibition. Studying the inhibition of A. flavus showed that the effect of SBTI/SBTI on A. flavus α-amylase activity and aflatoxin B 1 production depended on TI concentration. However, no inhibitory effect was observed when sufficient exogenous α-amylase (EC 3.2.1.1, from Bacillus subtilis) was added. The resistance to A. flavus infection was partially due to the ability of SBTI/WBTI to inhibit α-amylase activity, thereby limiting the availability of hydrolyzed reducing sugar for fungal growth and further suppressing aflatoxin B 1 biosynthesis. In addition, the relationship between SBTI/WBTI levels and fungal protease expression revealed that A. flavus released a certain quantity of endogenous proteases into the culture medium, and the decreased activity of protease and production of aflatoxin B 1 suggested that the inhibition activity might also be mediated by SBTI/WBTI as A. flavus protease inhibitor activity.

show abstract

Different factors affecting growth and amylase production by fungi inhabiting poultry feeds

Mahmoud

1993

J. Basic Microbiol.

View full text Add to dashboard Cite

Extracellular amylase synthesis by Aspergillus f l a w s and A. fumigatus, isolated from poultry feeds, was studied in stationary liquid medium. Maximum mycelium and extracellular amylase were produced after 7 days of incubation at 30 "C and after 5 days at 35 "C by A.flavus and A. fumigatus, respectively. Optimal amylase yield was obtained at pH 8 and 6 by the two fungi, respectively. Amylase production did not depend on the presence of starch in the growth medium but its inclusion in the medium enhanced amylase production by A . fumigatus. Sucrose appeared to be a poor carbon source for amylase synthesis by the test fungi. Growth on varying nitrogen sources showed that glycine promoted amylase production by A.flaous while yeast extract achieved maximum enzymatic yield by A . fumigatus.Microorganisms, including fungi, are responsible for the spoilage of agricultural products during storage (MULINGE and APINIS 1969). This is particularly pronounced when storage conditions are favourable for microbial colonization of such microorganisms (OGUNDERO 1983, MOHARRAM et al. 1989.Feeds stored under conditions encourage fungi development may adversely affect health and performance of animals (BARTOV et al. 1982). BEASLEY et al. (1980) found that chicks fed corn inoculated with Penicillium lanosum developed diarrhoea and grew at a slow rate than the controls.The deleterious effect of fungi contaminated feeds could be attributed to the presence of mycotoxins or decrease in their nutritional value (BARTOV etal. 1982). The possible effect of fungi on the nutritional value of feedstuffs has not received much attention. RICHARDSON et al. (1962) observed in poults receiving moldy soybean meal a growth depression that was prevented by the addition of lysine.The raltive ease these fungi carry out the biodegradation of feeds is directly linked to their capability to produce some extracellular enzymes. The proteolytic activity of fungi isolated from poultry feeds was previously studied in this laboratory (MEGALLA et al. 1990).The present study was designed to investigate the effect of environmental and nutritional factors on the capability of fungi inhabiting poultry feeds to produce amylase necessary for the hydrolysis of starch in these feeds. Materials and methodsOrganisms and culture maintenance: The two isolates of Aspergillusflavus and A. fumigatus used in this study were isolated from poultry feedstuffs (MOHARRAM et al. 1989). Preliminary studies had shown that, these two isolates produced appreciable amounts of amylase on solid synthetic starch yeast extract medium (SYEM). Cultures were maintained on CZAPEK'S yeast extract agar.Growth medium and amylase production: The test fungi were cultured in a liquid medium with a composition in g of soluble starch (MERCK), 5.0, yeast extract 2.0, KH,PO, 1.0, MgSO, . 7 H,O 0.5, distilled water 1 litre with a pH 7.5. This medium was autoclaved (121 "C for 15 min), allowed to 13'

show abstract

Mycelial Growth and Amylase Production by Talaromyces emersonii

Cited by 5 publications

References 11 publications

Post-harvest enzymatic activities of healthy and infected Cassava (Manihot esculenta Crantz) tubers

Post-harvest enzymatic activities of healthy and infected Cassava (Manihot esculenta Crantz) tubers

Inhibition of endogenous α-amylase and protease of Aspergillus flavus by trypsin inhibitor from cultivated and wild-type soybean

Different factors affecting growth and amylase production by fungi inhabiting poultry feeds

Contact Info

Product

Resources

About