Myc Target in Myeloid Cells-1, a Novel c-Myc Target, Recapitulates Multiple c-Myc Phenotypes

Yin, Xiaoying; Grove, Linnette; Rogulski, Kenneth; Prochownik, Edward V.

doi:10.1074/jbc.m200860200

Cited by 34 publications

(80 citation statements)

References 52 publications

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“…Previous study showed that the murine homologue of MT‐MC1 localized in the nucleus 13. In this study, we identified that human MYCT1 localized predominantly to the cytoplasmic vesicle complex although MYCT1 protein contains a putative NLS motif, which usually is a nuclear import signal.…”

Section: Discussionmentioning

confidence: 67%

“…It was reported that MT‐MC1 affected cell proliferation, morphology, apoptosis, genomic stability and differentiation 13. Liddiard et al .…”

Section: Discussionmentioning

confidence: 99%

“…Overexpression of murine MYCT1 (MT‐MC1) rescues multiple phenotypes of c‐Myc knockout mouse cell lines 11, 12. MT‐MC1 affects several cellular processes including cell cycle progression and apoptosis, cell transformation, cell differentiation and genomic instability 13. Yin et al .…”

Section: Introductionmentioning

confidence: 99%

“…Yin et al . analysed the sequence of MT‐MC1 protein and found a low conserved domain of a DNA helicase of virus and a putative nuclear localization sequence (NLS) 13. Rogulski et al .…”

Section: Introductionmentioning

confidence: 99%

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Transmembrane domain is crucial to the subcellular localization and function of Myc target 1

Gui

Yin

et al. 2015

J Cellular Molecular Medi

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Deregulation of c‐MYC occurs in a variety of human cancers. Overexpression of c‐MYC promotes cell growth, proliferation, apoptosis, transformation and genomic instability. MYC target 1 (MYCT1) is a direct target gene of c‐MYC, and its murine homologue MT‐MC1 recapitulated multiple c‐Myc‐related phenotypes. However, the molecular mechanism of MYCT1 remains unclear. Here, we identified the transmembrane (TM) domain of MYCT1, not the nuclear localization sequence, is indispensable to the vesicle‐associated localization of MYCT1 protein in the cytoplasmic membrane vesicle. Overexpression of MYCT1, not MYCT1 (ΔTM), decreased cell viability under serum deprivation and increased tumour cell migration ability. We further identified CKAP4 interacted with MYCT1 and contributed to the function of MYCT1. In addition, we found that a mutation, A88D, which is observed in patient sample, changed the localization, and abolished the effect on cell viability and cell migration, suggesting that the TM domain is critical to MYCT1.

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Section: Discussionmentioning

confidence: 67%

“…It was reported that MT‐MC1 affected cell proliferation, morphology, apoptosis, genomic stability and differentiation 13. Liddiard et al .…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Yin et al . analysed the sequence of MT‐MC1 protein and found a low conserved domain of a DNA helicase of virus and a putative nuclear localization sequence (NLS) 13. Rogulski et al .…”

Section: Introductionmentioning

confidence: 99%

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Transmembrane domain is crucial to the subcellular localization and function of Myc target 1

Gui

Yin

et al. 2015

J Cellular Molecular Medi

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“…One such target, MT-MC1, encodes a nuclear protein with the unique ability to reproduce multiple c-Myc functions (Yin et al, 2002;Rothermund et al, 2005). Surprisingly, MT-MC1 deregulates o50 genes most of which are also direct c-Myc targets (Rogulski et al, 2005b).…”

mentioning

confidence: 99%

Transformation, genomic instability and senescence mediated by platelet/megakaryocyte glycoprotein Ibα

Cohen

et al. 2007

Oncogene

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GpIba, a subunit of the von Willebrand factor receptor, functions during blood clotting to promote platelet adhesion and activation. GpIba is widely expressed, is positively regulated by c-Myc and is essential for the promotion of c-Myc-mediated chromosomal instability. We now show that GpIba is also a classical oncoprotein in which its deregulated expression leads to transformation, reduced growth factor requirements, increased resistance to apoptosis, and, in primary cells, p53-dependent senescence. Finally, GpIba also promotes double-stranded DNA breaks, and induces profound nuclear dysmorphology, indicating that, in addition to its direct transforming function, it displays genotoxicity at several distinct levels. These findings identify novel functions for GpIba and pathways through which c-Myc mediates transformation and global genomic destabilization.

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YY1 directly suppresses MYCT1 leading to laryngeal tumorigenesis and progress

Sun

et al. 2017

Cancer Medicine

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Human MYCT1 cDNA was first cloned using in silicon hybridization and molecular methods and previously AbstractYY1 is a key transcription factor and plays different roles in various cancers. However, role and mechanism of YY1 in laryngeal cancer are still unknown. YY1 and MYCT1 mRNA and protein levels were detected by Real-time RT-PCR and Western Blot methods, respectively. Binding of YY1 to MYCT1 promoter was predicted and confirmed by bioinformatics and chromatin immunoprecipitation assays, respectively. MYCT1 promoter activity was assessed by dual luciferase assay system. Laryngeal cancer cell proliferation, migration, and apoptosis were evaluated by cell viability, colony formation, cell scratch assay, transwell assay, and flow cytometry methods, respectively. YY1 and MYCT1 were upregulated and downregulated at transcriptional level in laryngeal cancer, respectively, which showed a negative correlation between YY1 and MYCT1 expression in laryngeal cancer. Significantly higher expression of YY1 and lower expression of MYCT1 were found in laryngeal cancer tissues of patients with lymphatic metastasis than those without metastasis.YY1 directly bound to MYCT1 promoter region and inhibited its promoter activity. YY1 silence had similar biological functions as MYCT1 overexpression in repressiveness of proliferation and migration, and promotion of apoptosis in laryngeal cancer cells. However, the effects of YY1 silence were recovered by MYCT1 knockdown. YY1 promotes proliferation and migration with suppression of apoptosis via directly inhibiting MYCT1 in laryngeal cancer cells, suggesting that YY1 is a useful target as a potential oncogene in laryngeal cancer development and progression. implying that MYCT1 function in cancer is tissue-specific. For example, MYCT1 is downregulated and promotes apoptosis in laryngeal and gastric cancer [1,2]. However, MYCT1 is upregulated and enhances cancer cell viability in acute myeloid leukemia [3]. Therefore, study on MYCT1 regulation network helps us to understand the tissuespecific manner of MYCT1 in cancer.At present, several transcription factors have been reported to be a direct regulator of MYCT1. In our and other studies, C-MYC directly targets MYCT1 promoter region and regulates its expression [1,[4][5][6]. We also found that MYCT1 promoter region including C-MYC binding site is hypermethylated in laryngeal cancer, suggesting that the methylation status interferes with the binding of C-MYC to MYCT1 leading to MYCT1 dysregulation in laryngeal cancer [7]. Liddiard et al. discovered that the fusion protein RUNX-ETO contributes to key aspects of the leukemic phenotypes via targeting MYCT1 [3]. In addition to C-MYC and RUNX-ETO, other transcription factors regulating MYCT1 expression are not reported.YY1 (Yin-Yang-1) is a zinc-finger transcription factor that belongs to the GLI-Krüppel gene family [8] and abnormally expressed in lots of cancer such as colon cancer [9], non-small-cell lung cancer [10], and myeloma [11], indicating that it has key roles in carcinogenesis. Ho...

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Myc Target in Myeloid Cells-1, a Novel c-Myc Target, Recapitulates Multiple c-Myc Phenotypes

Cited by 34 publications

References 52 publications

Transmembrane domain is crucial to the subcellular localization and function of Myc target 1

Transmembrane domain is crucial to the subcellular localization and function of Myc target 1

Transformation, genomic instability and senescence mediated by platelet/megakaryocyte glycoprotein Ibα

YY1 directly suppresses MYCT1 leading to laryngeal tumorigenesis and progress

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