MYBs Drive Novel Consumer Traits in Fruits and Vegetables

Allan, Andrew C.; Espley, Richard V.

doi:10.1016/j.tplants.2018.06.001

Cited by 125 publications

(96 citation statements)

References 105 publications

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“…Otherwise, miRNA play important roles in plant development process and response to environmental stresses by regulated the expression of transcription factors. Based on past researches, the expression level of miR159 and miR171 were closely related to fruit development [44,45]. Our sequencing results showed that the expression level of circRNA (chrChr6:38016308|38019170), the target of which is hr-miR159d, was significantly downregulated as fruit ripening.…”

Section: Disscusionmentioning

confidence: 73%

Identification and characterization of circular RNAs during the sea buckthorn fruit development

Zhang

Chen

et al. 2019

RNA Biology

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As a rising star of noncoding RNA, circular RNAs (circRNAs) have a covalently closed loop structure, which formed by 3ʹ-5ʹ ligation during splicing. A few circRNAs were identified and thought to be transcriptional noise due to cognitive defect over the past 40 years. Recently, with the development of high-throughput RNA sequencing techniques and specific algorithms for circRNA detection and quantification, plenty of potential circRNAs were identified in many species which play important roles in various biological processes. However, researches on circRNAs in fruit ripening process were lacking. Here, we totally identified 2616 circRNAs in sea buckthorn fruit development process, which uniformly distributed in sea buckthorn chromosome. Among them, 1721 (65.8%) circRNAs were arising from the exons of their host genes, 252 circRNAs were identified as the differentially expressed circRNAs (DEcircRNAs) between three different development stages, and 181 (71.8%) DEcircRNAs had sequence similarity with 235 identified circRNAs from five know plant species. Functional annotation revealed that host genes of DEcircRNAs were predicted to be involved in carotenoid biosynthesis, lipid synthesis and plant hormone signal transduction. Additionally, 53 DEcircRNAs were predicted as the corresponding nine miRNAs sponges in sea buckthorn. Divergent reverse-transcription PCR and RT-qPCR were used for validate the differential expression and back-splicing sites of six DEcircRNAs. These results revealed the role of circRNAs in sea buckthorn fruit ripening process and promoted the noncoding RNA researches in plants.

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Section: Disscusionmentioning

confidence: 73%

Identification and characterization of circular RNAs during the sea buckthorn fruit development

Zhang

Chen

et al. 2019

RNA Biology

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“…However, the overexpression of apple MdHY5 results in the overaccumulation of anthocyanin in apple calli (An et al ., ). In addition, ERF, NAC and BBX proteins are involved in the transcriptional regulation of MYB proteins (Allan and Espley, ).…”

Section: Introductionmentioning

confidence: 99%

BBX16, a B‐box protein, positively regulates light‐induced anthocyanin accumulation by activating MYB10 in red pear

Bai

Tao

Tang

et al. 2019

Plant Biotechnology Journal

225

180

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Summary The red coloration of pear (Pyrus pyrifolia) results from anthocyanin accumulation in the fruit peel. Light is required for anthocyanin biosynthesis in pear. A pear homolog of Arabidopsis thaliana BBX22, PpBBX16, was differentially expressed after fruits were removed from bags and may be involved in anthocyanin biosynthesis. Here, the expression and function of PpBBX16 were analysed. PpBBX16's expression was highly induced by white‐light irradiation, as was anthocyanin accumulation. PpBBX16's ectopic expression in Arabidopsis increased anthocyanin biosynthesis in the hypocotyls and tops of flower stalks. PpBBX16 was localized in the nucleus and showed trans‐activity in yeast cells. Although PpBBX16 could not directly bind to the promoter of PpMYB10 or PpCHS in yeast one‐hybrid assays, the complex of PpBBX16/PpHY5 strongly trans‐activated anthocyanin pathway genes in tobacco. PpBBX16's overexpression in pear calli enhanced the red coloration during light treatments. Additionally, PpBBX16's transient overexpression in pear peel increased anthocyanin accumulation, while virus‐induced gene silencing of PpBBX16 decreased anthocyanin accumulation. The expression patterns of pear BBX family members were analysed, and six additional BBX genes, which were differentially expressed during light‐induced anthocyanin biosynthesis, were identified. Thus, PpBBX16 is a positive regulator of light‐induced anthocyanin accumulation, but it could not directly induce the expression of the anthocyanin biosynthesis‐related genes by itself but needed PpHY5 to gain full function. Our work uncovered regulatory modes for PpBBX16 and suggested the potential functions of other pear BBX genes in the regulation of anthocyanin accumulation, thereby providing target genes for further studies on anthocyanin biosynthesis.

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“…Consumers demand healthy and novel fruits with good flavour (Allan & Espley ; Klee & Tieman ). Flavour, a complex perception of taste and aroma (Small ), is mainly driven by sugars, acids, and volatile compounds (Wang & Seymour ).…”

Section: Introductionmentioning

confidence: 99%

Carbon starvation reduces carbohydrate and anthocyanin accumulation in red‐fleshed fruit via trehalose 6‐phosphate and MYB27

Nardozza

Boldingh

Kashuba

et al. 2019

Plant Cell & Environment

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Kiwifruit (Actinidia spp.) is a recently domesticated fruit crop with several novel‐coloured cultivars being developed. Achieving uniform fruit flesh pigmentation in red genotypes is challenging. To investigate the cause of colour variation between fruits, we focused on a red‐fleshed Actinidia chinensis var. chinensis genotype. It was hypothesized that carbohydrate supply could be responsible for this variation. Early in fruit development, we imposed high or low (carbon starvation) carbohydrate supplies treatments; carbohydrate import or redistribution was controlled by applying a girdle at the shoot base. Carbon starvation affected fruit development as well as anthocyanin and carbohydrate metabolite concentrations, including the signalling molecule trehalose 6‐phosphate. RNA‐Seq analysis showed down‐regulation of both gene‐encoding enzymes in the anthocyanin and carbohydrate biosynthetic pathways. The catalytic trehalose 6‐phosphate synthase gene TPS1.1a was down‐regulated, whereas putative regulatory TPS7 and TPS11 were strongly up‐regulated. Unexpectedly, under carbon starvation MYB10, the anthocyanin pathway regulatory activator was slightly up‐regulated, whereas MYB27 was also up‐regulated and acts as a repressor. To link these two metabolic pathways, we propose a model where trehalose 6‐phosphate and the active repressor MYB27 are involved in sensing the carbon starvation status. This signals the plant to save resources and reduce the production of anthocyanin in fruits.

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MYBs Drive Novel Consumer Traits in Fruits and Vegetables

Cited by 125 publications

References 105 publications

Identification and characterization of circular RNAs during the sea buckthorn fruit development

Identification and characterization of circular RNAs during the sea buckthorn fruit development

BBX16, a B‐box protein, positively regulates light‐induced anthocyanin accumulation by activating MYB10 in red pear

Carbon starvation reduces carbohydrate and anthocyanin accumulation in red‐fleshed fruit via trehalose 6‐phosphate and MYB27

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