MYB75 Functions in Regulation of Secondary Cell Wall Formation in the Arabidopsis Inflorescence Stem

Bhargava, Apurva; Mansfield, Shawn D.; Hall, Hardy; Douglas, Carl J.; Ellis, Brian E.

doi:10.1104/pp.110.162735

Cited by 184 publications

(175 citation statements)

References 56 publications

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“…Lines segregating for fil-8, yab3-2, fil-8 yab3-2, yab2-1 yab3-2 yab5-1, and fil-8 yab2-1 yab3-2 yab5-1 have been described previously (Stahle et al, 2009). The myb75-1 mutant has been previously described (Bhargava et al, 2010). The 35S:MYC2-GFP and 35S:JAZ3ΔC-GFP lines were previously described (Lorenzo et al, 2004;Chini et al, 2007), as was the 35S: FIL-GR line (Bonaccorso et al, 2012).…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

FILAMENTOUS FLOWER Is a Direct Target of JAZ3 and Modulates Responses to Jasmonate

et al. 2015

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The plant hormone jasmonate (JA) plays an important role in regulating growth, development, and immunity. Activation of the JA-signaling pathway is based on the hormone-triggered ubiquitination and removal of transcriptional repressors (JASMONATE-ZIM DOMAIN [JAZ] proteins) by an SCF receptor complex (SCF COI1 /JAZ). This removal allows the rapid activation of transcription factors (TFs) triggering a multitude of downstream responses. Identification of TFs bound by the JAZ proteins is essential to better understand how the JA-signaling pathway modulates and integrates different responses. In this study, we found that the JAZ3 repressor physically interacts with the YABBY (YAB) family transcription factor FILAMENTOUS FLOWER (FIL)/YAB1. In Arabidopsis thaliana, FIL regulates developmental processes such as axial patterning and growth of lateral organs, shoot apical meristem activity, and inflorescence phyllotaxy. Phenotypic analysis of JA-regulated responses in loss-and gain-offunction FIL lines suggested that YABs function as transcriptional activators of JA-triggered responses. Moreover, we show that MYB75, a component of the WD-repeat/bHLH/MYB complex regulating anthocyanin production, is a direct transcriptional target of FIL. We propose that JAZ3 interacts with YABs to attenuate their transcriptional function. Upon perception of JA signal, degradation of JAZ3 by the SCF COI1 complex releases YABs to activate a subset of JA-regulated genes in leaves leading to anthocyanin accumulation, chlorophyll loss, and reduced bacterial defense.

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Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

FILAMENTOUS FLOWER Is a Direct Target of JAZ3 and Modulates Responses to Jasmonate

et al. 2015

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“…KNAT7 functions as a transcriptional repressor in protoplast transfection assays (Bhargava et al, 2010;Li et al, 2011). To test whether BLH6 has a similar function, we employed a protoplast transactivation system ( Figure 3A; Supplemental Figure 3A; Wang et al, 2007).…”

Section: Blh6 Is a Transcriptional Repressormentioning

confidence: 99%

“…Differences in gene expression, expressed as the fold change relative to the control, were calculated as described previously (Bhargava et al, 2010). Each measurement was performed as three replicates and was repeated in three biological replicates (independent pools of inflorescence stems from the appropriate genotypes).…”

Section: Total Rna Isolation and Quantitative Rt-pcr Analysismentioning

confidence: 99%

BEL1-LIKE HOMEODOMAIN6 and KNOTTED ARABIDOPSIS THALIANA7 Interact and Regulate Secondary Cell Wall Formation via Repression of REVOLUTA

et al. 2014

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The TALE homeodomain transcription factor KNOTTED ARABIDOPSIS THALIANA7 (KNAT7) is part of a regulatory network governing the commitment to secondary cell wall biosynthesis of Arabidopsis thaliana, where it contributes to negative regulation of this process. Here, we report that BLH6, a BELL1-LIKE HOMEODOMAIN protein, specifically interacts with KNAT7, and this interaction influences secondary cell wall development. BLH6 is a transcriptional repressor, and BLH6-KNAT7 physical interaction enhances KNAT7 and BLH6 repression activities. The overlapping expression patterns of BLH6 and KNAT7 and phenotypes of blh6, knat7, and blh6 knat7 loss-of-function mutants are consistent with the existence of a BLH6-KNAT7 heterodimer that represses commitment to secondary cell wall biosynthesis in interfascicular fibers. BLH6 and KNAT7 overexpression results in thinner interfascicular fiber secondary cell walls, phenotypes that are dependent on the interacting partner. A major impact of the loss of BLH6 and KNAT7 function is enhanced expression of the homeodomainleucine zipper transcription factor REVOLUTA/INTERFASCICULAR FIBERLESS1 (REV/IFL1). BLH6 and KNAT7 bind to the REV promoter and repress REV expression, while blh6 and knat7 interfascicular fiber secondary cell wall phenotypes are suppressed in blh6 rev and knat7 rev double mutants, suggesting that BLH6/KNAT7 signaling acts through REV as a direct target.

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“…2). The combination of pathway-specific transcriptional regulators (Ko et al 2009) e Genes reported to be involved in the secondary wall biosynthesis (Ko and Han, unpublished results;Bhargava et al 2010;Zhong et al 2008;Zhou et al 2009) Plant Biotechnol Rep (2011) 5:1-7 5…”

Section: Resultsmentioning

confidence: 98%

Biotechnological improvement of lignocellulosic feedstock for enhanced biofuel productivity and processing

Kim

Han

2010

Plant Biotechnol Rep

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Secondary walls have recently drawn research interest as a primary source of sugars for liquid biofuel production. Secondary walls are composed of a complex mixture of the structural polymers cellulose, hemicellulose, and lignin. A matrix of hemicellulose and lignin surrounds the cellulose component of the plant's cell wall in order to protect the cell from enzymatic attacks. Such resistance, along with the variability seen in the proportions of the major components of the mixture, presents process design and operating challenges to the bioconversion of lignocellulosic biomass to fuel. Expanding bioenergy production to the commercial scale will require a significant improvement in the growth of feedstock as well as in its quality. Plant biotechnology offers an efficient means to create ''targeted'' changes in the chemical and physical properties of the resulting biomass through pathway-specific manipulation of metabolisms. The successful use of the genetic engineering approach largely depends on the development of two enabling tools: (1) the discovery of regulatory genes involved in key pathways that determine the quantity and quality of the biomass, and (2) utility promoters that can drive the expression of the introduced genes in a highly controlled manner spatially and/or temporally. In this review, we summarize the current understanding of the transcriptional regulatory network that controls secondary wall biosynthesis and discuss experimental approaches to developing-xylem-specific utility promoters.

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MYB75 Functions in Regulation of Secondary Cell Wall Formation in the Arabidopsis Inflorescence Stem

Cited by 184 publications

References 56 publications

FILAMENTOUS FLOWER Is a Direct Target of JAZ3 and Modulates Responses to Jasmonate

FILAMENTOUS FLOWER Is a Direct Target of JAZ3 and Modulates Responses to Jasmonate

BEL1-LIKE HOMEODOMAIN6 and KNOTTED ARABIDOPSIS THALIANA7 Interact and Regulate Secondary Cell Wall Formation via Repression of REVOLUTA

Biotechnological improvement of lignocellulosic feedstock for enhanced biofuel productivity and processing

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