MYB3Rs, plant homologs of Myb oncoproteins, control cell cycle-regulated transcription and form DREAM-like complexes

Kobayashi, Kunikazu; Suzuki, Toshiya; Iwata, Eriko; Magyar, Zoltán; Bögre, László; Ito, Masaki

doi:10.1080/21541264.2015.1109746

Cited by 26 publications

(26 citation statements)

References 30 publications

(76 reference statements)

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“…The MYB3R family members, MYB3R1 and MYB3R4 transcriptionally activate many G2/M promoting genes (Haga et al, 2011). MYB3R3 and MYB3R5 negatively regulate transcription of the same genes (Kobayashi et al, 2015). However, MYB3R1 can also negatively regulate transcription, redundantly with MYB3R3 and MYB3R5 at G2/M (Kobayashi et al, 2015).…”

Section: Resultsmentioning

confidence: 99%

“…MYB3R3 and MYB3R5 negatively regulate transcription of the same genes (Kobayashi et al, 2015). However, MYB3R1 can also negatively regulate transcription, redundantly with MYB3R3 and MYB3R5 at G2/M (Kobayashi et al, 2015).…”

Section: Resultsmentioning

confidence: 99%

“…The high expression of two repressive MYB3R5 homologs ( MYB3R5_3 and MYB3R5_4 ) and three Wee1 homologs ( Wee1_1 , Wee1_2 and Wee1_3 ) in the EC (Fig. 7, Supplemental Table 16) suggests that it is unlikely the EC can progress through the G2/M transition (Kobayashi et al, 2015).…”

Section: Resultsmentioning

confidence: 99%

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Gene expression in isolated cowpea (Vigna unguiculata L. Walp) cells from meiosis to seed initiation

Gursanscky

Mazurkiewicz

Juranić

et al. 2020

Preprint

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Molecular knowledge of pathways regulating seed formation in legumes, remains scarce. Thirteen isolated cell-type transcriptomes were developed, spanning temporal events of male and female gametogenesis and seed initiation, to examine pathways involved in cowpea seed formation. In situ hybridization confirmed localization of in silico identified cell-specific genes, verifying transcriptome utility. Cowpea and Arabidopsis reproductive cells showed some conservation in regulators enabling cell-type expression as some cowpea cell-specific genes promoters and their Arabidopsis homologs directed expression to identical reproductive cell-types in transgenic plants. In silico analyses revealed gene expression similarities and differences with genes in pathways regulating reproductive events in other plants. Meiosis-related genes were expressed at mitotic stages of gametogenesis and during sporophytic development in cowpea. Plant hormone pathways showing preferential expression at particular reproductive stages were identified. Expression of epigenetic pathways, resembling those found in Arabidopsis, including microRNA mediated gene silencing, RNA directed DNA methylation and histone modification were associated with particular stages of male and female gametophyte development, suggesting roles in gametogenic cell specification and elaboration. Analyses of cell-cycle related gene expression in mature cowpea female gametophytes, indicated that the egg and central cell were arrested at the G1/S and G2/M cell cycle phases, respectively, prior to fertilization. Pre-fertilization female gametophyte arrest was characterized by barely detectable auxin biosynthesis gene expression levels, and elevated expression of genes involved in RNA-mediated gene silencing and histone modification. These transcriptomes provide a useful resource for additional interrogation to support functional analyses for development of higher yielding cowpea and syntenic legume crops. One sentence summary Transcriptomes from cowpea reproductive cell-types isolated in temporal sequence from meiosis to seed initiation revealed cell-type gene expression profiles enabling comparison with gene expression pathways known to function in cell specification and reproductive development in other plants.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Gene expression in isolated cowpea (Vigna unguiculata L. Walp) cells from meiosis to seed initiation

Gursanscky

Mazurkiewicz

Juranić

et al. 2020

Preprint

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“…To investigate the effect of E2FB loss of function during leaf development, we analyzed two e2fb T-DNA insertion mutant alleles, e2fb-1 (SALK_103138) and e2fb-2 (SALK_120959; Berckmans et al, 2011a;Kobayashi et al, 2015aKobayashi et al, , 2015b. The T-DNA insertions in these mutants are located just behind and within the E2FB dimerization domain, respectively (Supplemental Fig.…”

Section: The E2fb Mutant Has Increased Number Of Cells In Developing mentioning

confidence: 99%

“…In Arabidopsis, E2FC, RBR, and MYB3R3 (a repressortype MYB3R or Rep-MYB3R) are part of the DREAM complex with a repressive function that establishes quiescence (Kobayashi et al, 2015b). However, unique to plants is that the activator-type E2FB partners the mitosis-specific activator MYB3R4 (an Act-MYB3R) in another DREAM complex (Kobayashi et al, 2015a(Kobayashi et al, , 2015bHarashima and Sugimoto, 2016). This provides additional support for the mitotic role of E2FB.…”

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confidence: 97%

E2FB Interacts with RETINOBLASTOMA RELATED and Regulates Cell Proliferation during Leaf Development

et al. 2019

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0000-0001-8376-7220 (Z.M.).Cell cycle entry and quiescence are regulated by the E2F transcription factors in association with RETINOBLASTOMA-RELATED (RBR). E2FB is considered to be a transcriptional activator of cell cycle genes, but its function during development remains poorly understood. Here, by studying E2FB-RBR interaction, E2F target gene expression, and epidermal cell number and shape in e2fb mutant and overexpression lines during leaf development in Arabidopsis (Arabidopsis thaliana), we show that E2FB in association with RBR plays a role in the inhibition of cell proliferation to establish quiescence. In young leaves, both RBR and E2FB are abundant and form a repressor complex that is reinforced by an autoregulatory loop. Increased E2FB levels, either by expression driven by its own promoter or ectopically together with DIMERIZATION PARTNER A, further elevate the amount of this repressor complex, leading to reduced leaf cell number. Cell overproliferation in e2fb mutants and in plants overexpressing a truncated form of E2FB lacking the RBR binding domain strongly suggested that RBR repression specifically acts through E2FB. The increased number of small cells below the guard cells and of fully developed stomata indicated that meristemoids preferentially hyperproliferate. As leaf development progresses and cells differentiate, the amount of RBR and E2FB gradually declined. At this stage, elevation of E2FB level can overcome RBR repression, leading to reactivation of cell division in pavement cells. In summary, E2FB in association with RBR is central to regulating cell proliferation during organ development to determine final leaf cell number. 518

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Biosynthesis of riccionidins and marchantins is regulated by R2R3-MYB transcription factors in Marchantia polymorpha

et al. 2018

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R2R3-MYB transcription factors constitute the largest gene family among plant transcription factor families. They became largely divergent during the evolution of land plants and regulate various biological processes. The functions of R2R3-MYBs are mostly characterized in seed plants but are poorly understood in non-seed plants. Here, we examined the function of two R2R3-MYB genes of Marchantia polymorpha (Mapoly0073s0038 and Mapoly0006s0226) that are closely related to subgroup 4 of the R2R3-MYB family. We performed LC/MS/MS metabolomics, RNA-seq analysis and expression analysis in overexpressors and knockout mutants of MpMYB14 and MpMYB02. Overexpression of MpMYB14 remarkably increased the amount of riccionidins, which are specific anthocyanins in liverworts and a few flowering plants. In contrast, overexpression of MpMYB02 increased the amount of several marchantins, which are characteristic cyclic bis (bibenzyl ether) compounds in M. polymorpha and related liverworts. Knockouts of MpMYB14 and MpMYB02 abolished the accumulation of riccionidins and marchantins, respectively. The expression of MpMYB14 was up-regulated by UV-B irradiation, N deficiency, and NaCl treatment, whereas the expression of MpMYB02 was down-regulated by NaCl treatment. Our results suggest that the regulatory framework of phenolic metabolism by R2R3-MYB was already established in early land plants.

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MYB3Rs, plant homologs of Myb oncoproteins, control cell cycle-regulated transcription and form DREAM-like complexes

Cited by 26 publications

References 30 publications

Gene expression in isolated cowpea (Vigna unguiculata L. Walp) cells from meiosis to seed initiation

Gene expression in isolated cowpea (Vigna unguiculata L. Walp) cells from meiosis to seed initiation

E2FB Interacts with RETINOBLASTOMA RELATED and Regulates Cell Proliferation during Leaf Development

Biosynthesis of riccionidins and marchantins is regulated by R2R3-MYB transcription factors in Marchantia polymorpha

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