MYB118 Represses Endosperm Maturation in Seeds of <i>Arabidopsis</i>

Barthole, Guillaume; To, Alexandra; Marchive, Chloé; Brunaud, Véronique; Soubigou‐Taconnat, Ludivine; Berger, Nathalie; Dubreucq, Bertrand; Lepiniec, Loïc; Baud, Sébastien

doi:10.1105/tpc.114.130021

Cited by 63 publications

(96 citation statements)

References 56 publications

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“…In the LCM dataset, however, all four genes were called present and at least weakly expressed within the suspensor. We also compared the different suspensor data sets for presence of previously described endosperm-specific genes (Kinoshita et al, 1999(Kinoshita et al, , 2004Luo et al, 2000;Kang et al, 2008;Li et al, 2013;Barthole et al, 2014). The LCM results show all six genes tested as present, whereas our data indicate that only two out of six are also at least weakly expressed in the suspensor (supplementary material Table S10) and those two were also detected in a RNA-seq transcriptome analysis (Nodine and Bartel, 2012).…”

Section: Nuclear Transcriptomic Data As Proxy For Gene Expression Promentioning

confidence: 81%

Cell type-specific transcriptome analysis in the early Arabidopsis thaliana embryo

et al. 2014

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In multicellular organisms, cellular differences in gene activity are a prerequisite for differentiation and establishment of cell types. In order to study transcriptome profiles, specific cell types have to be isolated from a given tissue or even the whole organism. However, wholetranscriptome analysis of early embryos in flowering plants has been hampered by their size and inaccessibility. Here, we describe the purification of nuclear RNA from early stage Arabidopsis thaliana embryos using fluorescence-activated nuclear sorting (FANS) to generate expression profiles of early stages of the whole embryo, the proembryo and the suspensor. We validated our datasets of differentially expressed candidate genes by promoter-reporter gene fusions and in situ hybridization. Our study revealed that different classes of genes with respect to biological processes and molecular functions are preferentially expressed either in the proembryo or in the suspensor. This method can be used especially for tissues with a limited cell population and inaccessible tissue types. Furthermore, we provide a valuable resource for research on Arabidopsis early embryogenesis.

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Section: Nuclear Transcriptomic Data As Proxy For Gene Expression Promentioning

confidence: 81%

Cell type-specific transcriptome analysis in the early Arabidopsis thaliana embryo

et al. 2014

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“…For example, JcMYB017, JcMYB019, and JcMYB020, were specifically expressed in seed. These genes were assigned to the MIII subgroup (C41) along with AtMYB115 (AT5G40360) and AtMYB118/PGA37 (AT3G27785), AtMYB115 was thought to play roles in embryogenesis (Wang et al, 2009), and AtMYB118 repressed endosperm maturation in Arabidopsis (Barthole et al, 2014). Several members of C34 (subgroup 20) in Arabidopsis responded to the abiotic stressors: salt, dehydration, and phosphate-starving (Feller et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Genome-wide analysis of the MYB gene family in physic nut ( Jatropha curcas L.)

Zhou

Chen

et al. 2015

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The MYB proteins comprise one of the largest transcription factor families in plants, and play key roles in regulatory networks controlling development, metabolism, and stress responses. A total of 125 MYB genes (JcMYB) have been identified in the physic nut (Jatropha curcas L.) genome, including 120 2R-type MYB, 4 3R-MYB, and 1 4R-MYB genes. Based on exon-intron arrangement of MYBs from both lower (Physcomitrella patens) and higher (physic nut, Arabidopsis, and rice) plants, we can classify plant MYB genes into ten groups (MI-X), except for MIX genes which are nonexistent in higher plants. We also observed that MVIII genes may be one of the most ancient MYB types which consist of both R2R3- and 3R-MYB genes. Most MYB genes (76.8% in physic nut) belong to the MI group which can be divided into 34 subgroups. The JcMYB genes were nonrandomly distributed on its 11 linkage groups (LGs). The expansion of MYB genes across several subgroups was observed and resulted from genome triplication of ancient dicotyledons and from both ancient and recent tandem duplication events in the physic nut genome. The expression patterns of several MYB duplicates in the physic nut showed differences in four tissues (root, stem, leaf, and seed), and 34 MYB genes responded to at least one abiotic stressor (drought, salinity, phosphate starvation, and nitrogen starvation) in leaves and/or roots based on the data analysis of digital gene expression tags. Overexpression of the JcMYB001 gene in Arabidopsis increased its sensitivity to drought and salinity stresses.

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“…However, the most efficient way to engineer high vaccenic acid levels into a high-yielding crop like oilseed rape may be to redirect the endogenous biosynthetic pathway from the seed coat/aleurone layer into the embryonic tissue. This could be achieved by engineering increased expression of AAD2 and AAD3 in the embryo or manipulating the MYB118 regulatory network (Barthole et al, 2014).…”

Section: The Seed Coat/aleurone Layer Has a Distinct Lipid Compositionmentioning

confidence: 99%

Spatial and Temporal Mapping of Key Lipid Species in Brassica napus Seeds

et al. 2017

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The regulation of lipid synthesis in oil seeds is still not fully understood. Oilseed rape (Brassica napus) is the third most productive vegetable oil crop on the global market; therefore, increasing our understanding of lipid accumulation in oilseed rape seeds is of great economic, as well as intellectual, importance. Matrix-assisted laser/desorption ionization-mass spectrometry imaging (MALDI-MSI) is a technique that allows the mapping of metabolites directly onto intact biological tissues, giving a spatial context to metabolism. We have used MALDI-MSI to study the spatial distribution of two major lipid species, triacylglycerols and phosphatidylcholines. A dramatic, heterogenous landscape of molecular species was revealed, demonstrating significantly different lipid compositions between the various tissue types within the seed. The embryonic axis was found to be particularly enriched in palmitic acid, while the seed coat/aleurone layer accumulated vaccenic, linoleic, and a-linoleic acids. Furthermore, the lipid composition of the inner and outer cotyledons differed from each other, a remarkable discovery given the supposed identical functionality of these two tissues. Triacylglycerol and phosphatidylcholine molecular species distribution was analyzed through a developmental time series covering early seed lipid accumulation to seed maturity. The spatial patterning of lipid molecular species did not vary significantly during seed development. Data gathered using MALDI-MSI was verified through gas chromatography analysis of dissected seeds. The distinct lipid distribution profiles observed imply differential regulation of lipid metabolism between the different tissue types of the seed. Further understanding of this differential regulation will enhance efforts to improve oilseed rape productivity and quality.

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MYB118 Represses Endosperm Maturation in Seeds of Arabidopsis

Cited by 63 publications

References 56 publications

Cell type-specific transcriptome analysis in the early Arabidopsis thaliana embryo

Cell type-specific transcriptome analysis in the early Arabidopsis thaliana embryo

Genome-wide analysis of the MYB gene family in physic nut ( Jatropha curcas L.)

Spatial and Temporal Mapping of Key Lipid Species in Brassica napus Seeds

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