Myb -related genes of the Kyoho grape ( Vitis labruscana ) regulate anthocyanin biosynthesis

Kobayashi, Shozo; Ishimaru, Megumi; Hiraoka, Kentaro; Honda, C.

doi:10.1007/s00425-002-0830-5

Cited by 417 publications

(125 citation statements)

References 38 publications

(57 reference statements)

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“…In grapes, VvMYBA1 is widely considered to be involved in the regulation of VvUFGT [56]. VvMYBA1 was down-regulated fivefold in sunlight-excluded ‘Jingxiu’ berry skin (and undetectable with qRT-PCR), which is consistent with the finding for ‘Cabernet Sauvignon’ grapes [11].…”

Section: Resultssupporting

confidence: 84%

Genome-Wide Transcriptional Profiles of the Berry Skin of Two Red Grape Cultivars (Vitis vinifera) in Which Anthocyanin Synthesis Is Sunlight-Dependent or -Independent

Cao

Guan

et al. 2014

PLoS ONE

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Global gene expression was analyzed in the berry skin of two red grape cultivars, which can (‘Jingyan’) or cannot (‘Jingxiu’) synthesize anthocyanins after sunlight exclusion from fruit set until maturity. Gene transcripts responding to sunlight exclusion in ‘Jingyan’ were less complex than in ‘Jingxiu’; 528 genes were induced and 383 repressed in the former, whereas 2655 genes were induced and 205 suppressed in ‘Jingxiu’. They were regulated either in the same or opposing manner in the two cultivars, or in only one cultivar. In addition to VvUFGT and VvMYBA1, some candidate genes (e.g. AOMT, GST, and ANP) were identified which are probably involved in the differential responses of ‘Jingxiu’ and ‘Jingyan’ to sunlight exclusion. In addition, 26 MYB, 14 bHLH and 23 WD40 genes responded differently to sunlight exclusion in the two cultivars. Interestingly, all of the 189 genes classified as being relevant to ubiquitin-dependent protein degradation were down-regulated by sunlight exclusion in ‘Jingxiu’, but the majority (162) remained unchanged in ‘Jingyan’ berry skin. It would be of interest to determine the precise role of the ubiquitin pathway following sunlight exclusion, particularly the role of COP9 signalosome, cullins, RING-Box 1, and COP1-interacting proteins. Only a few genes in the light signal system were found to be regulated by sunlight exclusion in either or both cultivars. This study provides a valuable overview of the transcriptome changes and gives insight into the genetic background that may be responsible for sunlight-dependent versus -independent anthocyanin biosynthesis in berry skin.

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Section: Resultssupporting

confidence: 84%

Genome-Wide Transcriptional Profiles of the Berry Skin of Two Red Grape Cultivars (Vitis vinifera) in Which Anthocyanin Synthesis Is Sunlight-Dependent or -Independent

Cao

Guan

et al. 2014

PLoS ONE

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“…UDP-glucose:flavonol-3-O-glucosyltransferasae (UFGT, SSP:7411, AF000371) mRNA was also expressed specifically in the skin [51]. This enzyme plays a critical role in anthocyanin accumulation as retrotransposon-induced mutation of the MYB transcription factor (VvmybA1) gene [52] leading to its expression impairs anthocyanin accumulation in red grape varieties [53] with this mutation being common to many white grape varieties [5]. The enzymatic activity of recombinant UFGT has been documented in vitro [54].…”

Section: Discussionmentioning

confidence: 99%

Proteomic and selected metabolite analysis of grape berry tissues under well‐watered and water‐deficit stress conditions

et al. 2009

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In order to investigate the unique contribution of individual wine grape (Vitis vinifera) berry tissues and water-deficit to wine quality traits, a survey of tissue-specific differences in protein and selected metabolites was conducted using pericarp (skin and pulp) and seeds of berries from vines grown under well watered and water-deficit stress conditions. Of 1,047 proteins surveyed from pericarp by 2D-PAGE, 90 identified proteins showed differential expression between the skin and pulp. Of 695 proteins surveyed from seed tissue, 163 were identified and revealed that the seed and pericarp proteomes were nearly completely distinct from one another. Water-deficit stress altered the abundance of approximately 7% of pericarp proteins, but had little effect on seed protein expression. Comparison of protein and available mRNA expression patterns showed that 32% pericarp and 69% seed proteins exhibited similar quantitative expression patterns indicating that protein accumulation patterns are strongly influenced by post-transcriptional processes. About half of the 32 metabolites surveyed showed tissue-specific differences in abundance with water-deficit stress affecting the accumulation seven of these compounds. These results provide novel insights into the likely tissue-specific origins and the influence of water deficit stress on the accumulation of key flavor and aroma compounds in wine.

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“…In grapevine, most of the structural genes encoding the enzymes of the anthocyanins biosynthetic pathway have been identified (Sparvoli et al 1994;Boss et al 1996). Two adjacent transcription factors isolated from V. vinifera, VvmybA1 and VvmybA2, are involved in the control of the anthocyanins pathway (Kobayashi et al 2002;Kobayashi et al 2004;Walker et al 2007;Azuma et al 2008). The presence or absence of anthocyanins in grape berries segregates as a monogenic trait determined by a locus in linkage group 2 (Doligez et al 2002;Fischer et al 2004;Salmaso et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Phenotypic segregation and relationships of agronomic traits in Monastrell × Syrah wine grape progeny

et al. 2012

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The aim of this work was to study the phenotypic segregation of different agronomic and fruit quality traits, and their relationships, in Monastrell 9 Syrah wine grape progeny. Twenty-two agronomic traits were evaluated and compared for three consecutive years in this progeny. The results show the phenotypic diversity existing in a cross between two different wine grape cultivars. Most of the phenological, productive, morphological, and enological parameters evaluated displayed continuous variation within the progeny, suggesting a polygenic inheritance. Some correlations between traits were detected by the Spearman correlation test, although high coefficients were not found for most of them. Cluster analysis of the progeny grouped the hybrids based on criteria with significance for wine grape breeding. Also, we investigated the relationship between the skin color and total content of anthocyanins with the VvmybA genotype, using the CAPS (Cleaved Amplified Polymorphic Sequence) marker 20D18CB9. The results show that hybrids with two copies of the functional color allele tend to have increased anthocyanins content. Based on this study, 14 genotypes were pre-selected from the breeding population for additional quality studies.

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Myb -related genes of the Kyoho grape ( Vitis labruscana ) regulate anthocyanin biosynthesis

Cited by 417 publications

References 38 publications

Genome-Wide Transcriptional Profiles of the Berry Skin of Two Red Grape Cultivars (Vitis vinifera) in Which Anthocyanin Synthesis Is Sunlight-Dependent or -Independent

Genome-Wide Transcriptional Profiles of the Berry Skin of Two Red Grape Cultivars (Vitis vinifera) in Which Anthocyanin Synthesis Is Sunlight-Dependent or -Independent

Proteomic and selected metabolite analysis of grape berry tissues under well‐watered and water‐deficit stress conditions

Phenotypic segregation and relationships of agronomic traits in Monastrell × Syrah wine grape progeny

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