Myasthenia gravis: the specificities of skeletal muscle and thymus antibodies

Abstract

Antibodies against skeletal muscle antigens and against thymic myoid cells were examined in sera from 40 patients with myasthenia gravis (MG). Using an indirect immunofluorescence technique, antibodies against the surface of muscle cells were found in 23 sera, and antibodies against muscle cell cross-striations in 28 sera. Antibodies against thymic myoid cells were found in 27 sera, stained cells also occurring in fetal thymus from 14 weeks of gestation and in hyperplastic thymus from MG patients. Sera which stained myoid cells also stained muscle cell cross-striations. Sera from all the 20 patients with thymoma contained antibodies to a citric acid extract of skeletal muscle (CAE) as detected by indirect haemagglutination, whereas sera from 20 comparable patients without thymoma did not contain CAE antibodies. The myoid cell antibodies could be absorbed by homogenized muscle, but not by CAE-coated sheep erythrocytes. Different antibody patterns are seen among different MG patients; close associations were found between cross-striational and myoid cell antibodies, and between CAE-antibodies and a thymoma.

Muscle autoantibodies in myasthenia gravis: beyond diagnosis?

“…Virtually 100% of patients with thymomatous MG have detectable serum AChR antibodies [19]. The antibodies and their epitopes differ in their fine specificities from those in MG patients without thymoma [20,21]. A number of other muscle autoantibodies are also associated with thymoma and will be discussed below.…”