MXene Coupled with CRISPR-Cas12a for Analysis of Endotoxin and Bacteria

Sheng, Anzhi; Wang, Pei; Yang, Jingyi; Tang, Longfei; Chen, Feng; Zhang, Juan

doi:10.1021/acs.analchem.1c00371

Cited by 93 publications

(40 citation statements)

References 43 publications

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“…As a result, the designed fluorescence biosensor can efficiently determine DNA and other biomolecules 1381 . Similarly, Ti3C2 nanosheets 1382 , MXenes 1383 , BP 1384 With the help of catalyzed hairpin assembly (CHA) reaction, the detection limit of this sensing platform was down to 75.6 amol•L −1 , which is much lower than other similar detection strategies 1387 .…”

Section: Fluorescence Sensing Platformsmentioning

confidence: 90%

Recent Progress on Two-Dimensional Materials

Chang¹,

Chen²,

Chen³

et al. 2021

Acta Physico Chimica Sinica

311

119

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Research on two-dimensional (2D) materials has been explosively increasing in last seventeen years in varying subjects including condensed matter physics, electronic engineering, materials science, and chemistry since the mechanical exfoliation of graphene in 2004. Starting from graphene, 2D materials now have become a big family with numerous members and diverse categories. The unique structural features and physicochemical properties of 2D materials make them one class of the most appealing candidates for a wide range of potential applications.In particular, we have seen some major breakthroughs made in the field of 2D materials in last five years not only in developing novel synthetic methods and exploring new structures/properties but also in identifying innovative applications and pushing forward commercialisation. In this review, we provide a critical summary on the recent progress made in the field of 2D materials with a particular focus on last five years. After a brief background 物理化学学报 Acta Phys. -Chim. Sin. 2021, 37 (12), 2108017 (3 of 151) introduction, we first discuss the major synthetic methods for 2D materials, including the mechanical exfoliation, liquid exfoliation, vapor phase deposition, and wet-chemical synthesis as well as phase engineering of 2D materials belonging to the field of phase engineering of nanomaterials (PEN). We then introduce the superconducting/optical/magnetic properties and chirality of 2D materials along with newly emerging magic angle 2D superlattices. Following that, the promising applications of 2D materials in electronics, optoelectronics, catalysis, energy storage, solar cells, biomedicine, sensors, environments, etc. are described sequentially. Thereafter, we present the theoretic calculations and simulations of 2D materials. Finally, after concluding the current progress, we provide some personal discussions on the existing challenges and future outlooks in this rapidly developing field.

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Section: Fluorescence Sensing Platformsmentioning

confidence: 90%

Recent Progress on Two-Dimensional Materials

Chang¹,

Chen²,

Chen³

et al. 2021

Acta Physico Chimica Sinica

311

119

View full text Add to dashboard Cite

show abstract

“…2 F). This method can quantify the selectivity and sensitivity of endotoxin and gram-negative bacteria in different samples, and the detection limits are 11 pg/ml and 23 CFU/mL, respectively ( Sheng et al, 2021 ).…”

Section: Biosensing Technology Based On Crispr-cas12amentioning

confidence: 99%

“…(D) Detection mechanism of functional DNA-regulated CRISPR-Cas12a sensor for detecting non-nucleic acid targets ( Xiong et al, 2020 ). (E) The principle of citrinin analysis based on CRISPR-Cas12a ( Zhang et al, 2021a ) (F) Principle of endotoxin analysis based on CRISPR-Cas12a and MXene ( Sheng et al, 2021 ). …”

Section: Introductionmentioning

confidence: 99%

CRISPR/Cas12a-based technology: A powerful tool for biosensing in food safety

Mao

Chen

Wang

et al. 2022

Trends in Food Science & Technology

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“…Along with foodborne pathogens, contaminants such as heavy metals, pesticides, and other undesirable compounds can be added to food before it reaches the consumer. As we have seen, CRISPR‐based sensors’ targets are not restricted to nucleic acid (Abnous et al., 2021; Shen et al., 2020; Sheng et al., 2021), so all food contaminants have the potential to be their detection targets. A few studies have already tested this potential.…”

Section: Crispr‐based Sensing Systems In the Field Of Food Science: C...mentioning

confidence: 99%

Recent advances in CRISPR‐based systems for the detection of foodborne pathogens

Shin

Miller

Wang

2022

Comp Rev Food Sci Food Safe

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There has long been a need for more advanced forms of pathogen detection in the food industry. Though in its infancy, biosensing based on clustered regularly interspaced short palindromic repeats (CRISPR) has the potential to solve many problems that cannot be addressed using conventional methods. In this review, we briefly introduce and classify the various CRISPR/Cas protein effectors that have thus far been used in biosensors. We then assess the current state of CRISPR technology in food-safety contexts; describe how each Cas effector is utilized in foodborne-pathogen detection; and discuss the limitations of the current technology, as well as how it might usefully be applied in other areas of the food industry. We conclude that, if the limitations of existing CRISPR/Cas-based detection methods are overcome, they can be deployed on a wide scale and produce a range of positive food-safety outcomes.

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MXene Coupled with CRISPR-Cas12a for Analysis of Endotoxin and Bacteria

Cited by 93 publications

References 43 publications

Recent Progress on Two-Dimensional Materials

Recent Progress on Two-Dimensional Materials

CRISPR/Cas12a-based technology: A powerful tool for biosensing in food safety

Recent advances in CRISPR‐based systems for the detection of foodborne pathogens

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