MVA recombinants expressing the fusion and hemagglutinin genes of PPRV protects goats against virulent challenge

Chandran, Dev; Reddy, Kolli Bhaktavatsala; Vijayan, Shahana Pallichera; Sugumar, Parthasarthy; Rani, Gudavalli Sudha; Kumar, Ponsekaran Santha; Lingala, Rajendra; Srinivasan, V.A.

doi:10.1007/s12088-010-0026-9

Cited by 19 publications

(14 citation statements)

References 32 publications

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“…Unlike for NDV, goats might exhibit a pre-existing CPV immunity that interfered with the vaccine virus and required a booster vaccination to maintain protection from PPR [ 28 , 29 ]. Furthermore, vaccination of goats with a replication-defective human adenovirus or a recombinant vaccinia virus (MVA) that expresses PPRV H protected them from a lethal PPRV infection [ 30 , 35 ]. Two other vaccination studies demonstrated the induction of antibodies after a prime-boost vaccination i.m.…”

Section: Discussionmentioning

confidence: 99%

“…This can be achieved by the commercially available competitive PPR-N-ELISA [ 25 ]. Vaccination and challenge infection studies in sheep or goats report about the use of either capripoxviruses [ 26 , 27 , 28 , 29 ], adenoviruses [ 30 , 31 , 32 , 33 , 34 ], or vaccinia viruses [ 35 ] as vectors. However, none has been licensed for use so far.…”

Section: Introductionmentioning

confidence: 99%

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A Novel Recombinant Newcastle Disease Virus Vectored DIVA Vaccine against Peste des Petits Ruminants in Goats

et al. 2020

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Peste des petits ruminants virus (PPRV, species: small ruminant morbillivirus) is the causative agent of the eponymous notifiable disease, the peste des petits ruminants (PPR) in wild and domestic sheep and goats. Mortality rates vary between 50% and 100%, causing significant losses of estimated 1.5 to 2 billion US Dollars per year. Live-attenuated PPRV vaccine strains are used in the field for disease prevention, but the application of a more thermostable vaccine enabling differentiation between infected and vaccinated animals (DIVA) would be highly desirable to achieve the goal of global disease eradication. We generated a recombinant Newcastle disease virus (rNDV) based on the live-attenuated NDV Clone 30 that expresses the surface protein hemagglutinin (H) of PPRV strain Kurdistan/11 (rNDV_HKur). In vitro analyses confirmed transgene expression as well as virus replication in avian, caprine, and ovine cells. Two consecutive subcutaneous vaccinations of German domestic goats with rNDV_HKur prevented clinical signs and hematogenic dissemination after an intranasal challenge with virulent PPRV Kurdistan/11. Virus shedding by different routes was reduced to a similar extent as after vaccination with the live-attenuated PPRV strain Nigeria 75/1. Goats that were either not vaccinated or inoculated with parental rNDV were used as controls. In summary, we demonstrate in a proof-of-concept study that an NDV vectored vaccine can protect against PPR. Furthermore, it provides DIVA-applicability and a high thermal tolerance.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Novel Recombinant Newcastle Disease Virus Vectored DIVA Vaccine against Peste des Petits Ruminants in Goats

et al. 2020

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“…The vaccinia virus vector (Modified vaccinia Ankara) expressing Peste des petits ruminants virus F and H proteins was shown to protect goats against the disease after the administration of two doses of the vaccine ( Chandran et al, 2010 ), which might decrease its utility as a vaccine candidate. Goats immunised with a vaccine containing recombinant fowl pox virus expressing the H or F proteins of Peste des petits ruminants virus had a poor antibody response to the heterologous proteins ( Herbert et al, 2014 ).…”

Section: Controlmentioning

confidence: 99%

Peste des petits ruminants

Parida

Muniraju

Mahapatra

et al. 2015

Veterinary Microbiology

192

198

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Peste des petits ruminants virus causes a highly infectious disease of small ruminants that is endemic across Africa, the Middle East and large regions of Asia. The virus is considered to be a major obstacle to the development of sustainable agriculture across the developing world and has recently been targeted by the World Organisation for Animal Health (OIE) and the Food and Agriculture Organisation (FAO) for eradication with the aim of global elimination of the disease by 2030. Fundamentally, the vaccines required to successfully achieve this goal are currently available, but the availability of novel vaccine preparations to also fulfill the requisite for differentiation between infected and vaccinated animals (DIVA) may reduce the time taken and the financial costs of serological surveillance in the later stages of any eradication campaign. Here, we overview what is currently known about the virus, with reference to its origin, updated global circulation, molecular evolution, diagnostic tools and vaccines currently available to combat the disease. Further, we comment on recent developments in our knowledge of various recombinant vaccines and on the potential for the development of novel multivalent vaccines for small ruminants.

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“…Developing genetically marked vaccines would be advantageous during the eradication phase. Subunit vaccines prepared by expressing the PPRV F and/or H gene in pox and adeno viral vectors found to be experimentally protective (Caufour et al, 2014;Chandran et al, 2010;Chen et al, 2010;Rojas et al, 2014). Attempt was made to develop a subunit vaccine by expressing the H protein of PPRV in peanut plants (Arachis hypogea) and found to elicit neutralizing antibody responses in sheep (Khandelwal et al, 2011).…”

Section: Vaccinementioning

confidence: 99%

Peste-Des-Petits-Ruminants: An Indian Perspective

Muthuchelvan¹

2015

Adv. Anim. Vet. Sci.

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MVA recombinants expressing the fusion and hemagglutinin genes of PPRV protects goats against virulent challenge

Cited by 19 publications

References 32 publications

A Novel Recombinant Newcastle Disease Virus Vectored DIVA Vaccine against Peste des Petits Ruminants in Goats

A Novel Recombinant Newcastle Disease Virus Vectored DIVA Vaccine against Peste des Petits Ruminants in Goats

Peste des petits ruminants

Peste-Des-Petits-Ruminants: An Indian Perspective

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