Mutual Phenotypic Changes between Connective Tissue Type and Mucosal Mast Cells

Kitamura, Yukihiko; Kanakura, Yuzuru; Sonoda, S; Asai, Hidekazu; Nakano, Toru

doi:10.1159/000234198

Cited by 47 publications

(30 citation statements)

References 19 publications

(33 reference statements)

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“…However, BMMCs only marginally produce IL-1␤ mRNA and no TNF-␣ mRNA upon treatment with imiquimod in vitro. To explain this discrepancy, it is reasonable to assume that mast cells are able to change their characteristics following transfer in vivo, determined by the local microenvironment (30).…”

Section: Nfatc2 Expressed In Mast Cells Is Critical To Initiate Inflamentioning

confidence: 99%

Impaired Mast Cell-Driven Immune Responses in Mice Lacking the Transcription Factor NFATc2

Becker

Heib

Klein

et al. 2009

The Journal of Immunology

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The three calcium-dependent factors NFATc1, c2, and c3 are expressed in cells of the immune system and play pivotal roles in modulating cellular activation. With regard to NFATc2, it was reported that NFATc2-deficient mice display increased immune responses in several models for infection and allergy in vivo. This led to the assumption that NFATc2 is involved in the maintenance of immune homeostasis. Using the synthetic TLR7 agonist imiquimod as an adjuvant in epicutaneous peptide immunization, we observed that both the inflammatory reaction and the peptide-specific CTL response are severely impaired in NFATc2-deficient mice. Detailed analyses revealed that early production of proinflammatory cytokines, lymph node hypertrophy, and migration of Langerhans cells are strongly reduced in NFATc2-deficient animals. With the aid of mast cell-deficient mice and reconstitution experiments using mast cells derived from either NFATc2-deficient mice or wild-type controls, we were able to show that NFATc2 expressed in mast cells is critical for the initiation of inflammation, migration of Langerhans cells, and the development of full-blown CTL responses following epicutaneous immunization. Thus, NFATc2 is an important factor controlling mast cell accessory function at the interface of innate and adaptive immunity.

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Section: Nfatc2 Expressed In Mast Cells Is Critical To Initiate Inflamentioning

confidence: 99%

Impaired Mast Cell-Driven Immune Responses in Mice Lacking the Transcription Factor NFATc2

Becker

Heib

Klein

et al. 2009

The Journal of Immunology

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“…Mast hücreleri bulundukları yere ve granüllerindeki kimyasal içeriklerine göre Mukozal Mast Hücreleri (MMC) ve Konnektif Doku Mast Hücreleri (CTMC) olmak üzere ikiye ayrılmaktadır 5 .…”

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Morfological and Histometric Studies on Mast Cell Distribution and Heterogeneity, Present in the Lower Respiratory Tract and in the Lung of Local Duck (Anas platyrhnchase) and Goose (Anser anser)

Uslu¹,

Yörük²

2013

Kafkas Univ Vet Fak Derg

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ÖzetBu çalışma ördek ve kazlarda alt solunum yolları ve akciğerde bulunan mast hücrelerinin dağılımı ve heterojenitesini belirlemek amacıyla yapıldı. Çalışmada 6 adet kaz ve 6 adet ördek kullanıldı. Kanatlı hayvanların solunum sisteminde bulunan trakeya, syrinks, bronş ve akciğerden uygun büyüklükte parçalar alındı. BLA (Basic Kurşun Asetat -Mota), Carnoy, IFAA (Izotonik Formaldehit Asetik Asit)'da tespit edildikten sonra rutin doku takibi yapılarak paraplast ile bloklandı. 6-7 µm kalınlığında alınan kesitler %0.5'lik toluidine blue ve alcian blue-safranin O (AB/SO) kombine boyalarında boyandılar. Toluidine blue ile boyanan kesitlerde 40'lık objektifte 1mm 2 'lik alandaki mast hücre sayıları belirlendi. Her iki hayvan türünde de tespitlere göre değişmekle beraber incelenen organlardan akciğerde en fazla sayıda mast hücresine rastlandı. Sayısal veriler için en uygun tespitin IFAA olduğu, granül yapısını belirlemek için de en uygun tespitin BLA olduğu saptandı. Alcian blue-safranin O boyamasında ise kazlarda trakeya, syrinks, bronş ve akciğerde SO (+), AB (+) ve miks granüllü mast hücrelerine rastlanırken, ördeklerde trakeyada AB (+) hücreler, syrinks, bronş ve akciğerde de AB (+), SO (+), miks özellikte mast hücreleri görüldü. SummaryThis study was performed to determine mast cell distribution and heterogeneity present in the lower respiratory tract and in the lung of duck and goose. In this study, 6 ducks and 6 goose were used. Sufficient amount of tissue pieces from trachea, syrinks, bronches and lungs were taken from the choosen poultries. The samples were fixed in BLA (Basic Lead Acetate -Mota), Carnoy, IFAA (Isotonic Formaldehyde Acetic Acid), then, after routin tissue follow up, the samples were blocked with paraplast. Obtained sections (6-7 µm) were stained with 0.5% toluidine blue and alcian blue -safranin O (AB/SO). Sections stained with toloudine blue were examined under microscope (40 objective) and the number of mast cell in 1 mm 2 were determined. In both animal species; number of mast cells were changable according to fixation technique, with the highest number in lung samples. For numerical results; the most appropriate fixation technique was IFAA and BLA respectively. In AB/SO staining in trachea, syrinks bronches and lungs of geese, mast cells with SO (+), AB (+) and mix granules were obserwed. On the other hand; with the same staining in ducks; AB (+) cells in trachea, AB (+), SO (+) and mix charecteristic mast cells in syrinks, bronch and lungs, were obserwed. Keywords

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“…A number of other factors can act on mast cells to cause the release of these mediators, including neurotransmitters and steroid hormones (Johnson and Krenger, 1992;Arvy, 1955;Befus, 1990;Vliagoftis et al, 1990). Mast cells are highly "plastic" and can change secretory properties depending on their environment (Kitamura et al, 1987a(Kitamura et al, , 1987b, making them ideal for delivering biologically active substances to discrete regions in the brain and body on demand. The observation that mast cells are mobile immune cells located within both the CNS and periphery, coupled with evidence that steroid hormones and neurotransmitters can act on mast cells to modulate the release of their contents, suggests that mast cells may represent an important locus for neuroendocrine-immune interactions .…”

Section: Introductionmentioning

confidence: 99%

Brain mast cells are influenced by chemosensory cues associated with estrus induction in female prairie voles (Microtus ochrogaster)

Kriegsfeld

Hotchkiss

Demas

et al. 2003

Hormones and Behavior

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Historically, the brain has been viewed as protected from the infiltration of peripheral hematopoietic cells by the blood-brain barrier. However, numerous immune cell types have been found in the central nervous system (CNS). Mast cells, granulocytic immune cells, are found in the CNS of birds and mammals and their numbers and location are influenced by both extrinsic and intrinsic factors, including reproductive behavior and endocrine status. The present study used female prairie voles (Microtus ochrogaster) to investigate the interactions between brain mast cells and stimuli associated with estrus induction. Unlike spontaneous ovulators such as rats and mice, female prairie voles are induced into estrus by chemosensory stimuli present in conspecific male urine. Prior to estrus induction, female voles have undetectable concentrations of estrogen that rise rapidly following exposure to a male or male urine. In the first experiment, we examined whether mast cells may be influenced by estrus induction. Female voles exposed to conspecific male urine had increased numbers of mast cells in the main olfactory bulbs and epithalamus (medial habenula), but not the thalamus or median eminence, relative to control groups. Next, to determine if this mast cell increase was the result of elevated estrogen concentrations, female voles were injected with estradiol or vehicle and brain mast cell numbers analyzed. No differences in brain mast cell numbers were observed between estradiol-injected and control females in any brain area investigated. Together, these results lend further support to the contention that mast cell numbers and/or distribution can be influenced by reproductively relevant stimuli and underscore the utility of this vole model for delineating the function of brain mast cells.

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Mutual Phenotypic Changes between Connective Tissue Type and Mucosal Mast Cells

Cited by 47 publications

References 19 publications

Impaired Mast Cell-Driven Immune Responses in Mice Lacking the Transcription Factor NFATc2

Impaired Mast Cell-Driven Immune Responses in Mice Lacking the Transcription Factor NFATc2

Morfological and Histometric Studies on Mast Cell Distribution and Heterogeneity, Present in the Lower Respiratory Tract and in the Lung of Local Duck (Anas platyrhnchase) and Goose (Anser anser)

Brain mast cells are influenced by chemosensory cues associated with estrus induction in female prairie voles (Microtus ochrogaster)

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