Mutual interaction of red blood cells influenced by nanoparticles

Avsievich, Tatiana; Попов, А. П.; Bykov, Alexander; Meglinski, Igor

doi:10.1038/s41598-019-41643-x

Cited by 46 publications

(51 citation statements)

References 27 publications

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“…This dependence is typical for the so called "cross-bridges" model, assuming the accumulation of "cross-bridges" formed by plasma proteins between the interacting RBCs. RBCs interaction energy always increases upon cell disaggregation, as it was confirmed before in our studies [50,55,56]. Here, RBCs in presence of all the types of NCs demonstrate the same dependency, suggesting no difference in the interaction mechanism between the cells.…”

Section: Resultssupporting

confidence: 88%

Impact of Nanocapsules on Red Blood Cells Interplay Jointly Assessed by Optical Tweezers and Microscopy

et al. 2019

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In the framework of novel medical paradigm the red blood cells (RBCs) have a great potential to be used as drug delivery carriers. This approach requires an ultimate understanding of the peculiarities of mutual interaction of RBC influenced by nano-materials composed the drugs. Optical tweezers (OT) is widely used to explore mechanisms of cells’ interaction with the ability to trap non-invasively, manipulate and displace living cells with a notably high accuracy. In the current study, the mutual interaction of RBC with polymeric nano-capsules (NCs) is investigated utilizing a two-channel OT system. The obtained results suggest that, in the presence of NCs, the RBC aggregation in plasma satisfies the ‘cross-bridges’ model. Complementarily, the allocation of NCs on the RBC membrane was observed by scanning electron microscopy (SEM), while for assessment of NCs-induced morphological changes the tests with the human mesenchymal stem cells (hMSC) was performed. The combined application of OT and advanced microscopy approaches brings new insights into the conception of direct observation of cells interaction influenced by NCs for the estimation of possible cytotoxic effects.

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Section: Resultssupporting

confidence: 88%

Impact of Nanocapsules on Red Blood Cells Interplay Jointly Assessed by Optical Tweezers and Microscopy

et al. 2019

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“…The total surface area of a single RBC is denoted by S0. Force calibration based on force equilibrium between the optical trapping force, which is proportional to the trapping power, and the viscous friction force exerted on a trapped RBC by a counterflow with a known velocity is necessary prior to experiments 10 . In our case, the linear relationship between the optical trapping force and the trapping power was obtained as Ftrap = 0.2359*P -0.6684.…”

Section: Methodsmentioning

confidence: 99%

Influence of interaction time on the red blood cell (dis)aggregation dynamics in vitro studied by optical tweezers

Zhu

Avsievich

Попов

et al. 2019

Novel Biophotonics Techniques and Applications V

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Optical tweezers (OT) is a unique Nobel Prize winning technology that has been widely used in studying cell interaction dynamics at a single-cell level with highly accurate manipulating of living cells and the ability to detect ultra-low intercellular forces. The reversible aggregation process of red blood cells (RBCs) that strongly influences the blood rheological properties thus being critical for blood microcirculation has long been research studied. However, in spite of plentiful researches dealing with RBC aggregation behavior based on an average response of a large number of cells, the detailed mechanism and the applicability of the two coexistent yet mutually opposed models to this reversible process require additional information. In this study, a two-channel optical tweezers system is utilized to reveal the influence of the cell interaction time (0-300 sec) on the RBC (dis)aggregation dynamics. The results show that for RBC enforced disaggregation in autologous plasma, the longer the two RBCs adhere to each other, the stronger the intercellular interaction is, and the lower the degree that a certain optical pulling force can separate two cells, whereas no significant effect of cell interaction time on RBC aggregation process was observed. This observation indicates that the RBC aggregation and disaggregation in autologous plasma are governed by different mechanisms and that the hysteresis effect, namely the dependence of the disaggregation force on RBC interaction history, is a significant feature that needs special consideration in researches related to the RBC disaggregation process.

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“…A recent study from our group has examined the influence of different inorganic and polymeric nanoparticles on the RBC interaction dynamics. Among all of the tested nanoparticles, only the nanodiamonds induced an increase in the size of RBC aggregates and the aggregation force [127]. Such tests have practical significance for ensuring the safe use of novel nanoparticles in biomedical applications, including nanomedicine and photodynamic therapy (PDT).…”

Section: Applications Of Conventional and Polarized Otsmentioning

confidence: 96%

Optical Tweezers in Studies of Red Blood Cells

Zhu

Avsievich

Попов

et al. 2020

Cells

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Optical tweezers (OTs) are innovative instruments utilized for the manipulation of microscopic biological objects of interest. Rapid improvements in precision and degree of freedom of multichannel and multifunctional OTs have ushered in a new era of studies in basic physical and chemical properties of living tissues and unknown biomechanics in biological processes. Nowadays, OTs are used extensively for studying living cells and have initiated far-reaching influence in various fundamental studies in life sciences. There is also a high potential for using OTs in haemorheology, investigations of blood microcirculation and the mutual interplay of blood cells. In fact, in spite of their great promise in the application of OTs-based approaches for the study of blood, cell formation and maturation in erythropoiesis have not been fully explored. In this review, the background of OTs, their state-of-the-art applications in exploring single-cell level characteristics and bio-rheological properties of mature red blood cells (RBCs) as well as the OTs-assisted studies on erythropoiesis are summarized and presented. The advance developments and future perspectives of the OTs’ application in haemorheology both for fundamental and practical in-depth studies of RBCs formation, functional diagnostics and therapeutic needs are highlighted.

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Mutual interaction of red blood cells influenced by nanoparticles

Cited by 46 publications

References 27 publications

Impact of Nanocapsules on Red Blood Cells Interplay Jointly Assessed by Optical Tweezers and Microscopy

Impact of Nanocapsules on Red Blood Cells Interplay Jointly Assessed by Optical Tweezers and Microscopy

Influence of interaction time on the red blood cell (dis)aggregation dynamics in vitro studied by optical tweezers

Optical Tweezers in Studies of Red Blood Cells

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