Mutual Influences of<i>Pseudomonas aeruginosa</i>and<i>Desulfovibrio desulfuricans</i>on their Adhesion to Stainless Steel

Medilanski, Edi; Wick, Lukas Y.; Wanner, Oskar; Harms, Hauke

doi:10.1080/0892701021000030133

Cited by 8 publications

(3 citation statements)

References 18 publications

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“…Upon comparison of bacterial retention behavior between the batch and column assays, we found that retention of the cells was much greater in the batch assays, with ␣ values two to three times higher in the batch compared to the column studies. This is in contrast to prior research, which has shown that bacterial transport is much greater under convective (column) conditions, compared to the quiescent conditions of a batch experiment (Medilanski et al, 2003;Rijnaarts et al, 1993). A possible explanation for the discrepancy is that the bacteria have a greater affinity for glass than for the sand grains, so hydrodynamics alone cannot account for the differences in retention behavior.…”

Section: Bacterial Retention In Batch and Column Assayscontrasting

confidence: 79%

“…This simplification was made because this reaction time resulted in a moderate number of bacterial cells per field, which could be easily and reproducibly counted under the microscope. In addition, the 120-min reaction time was modeled after similar experiments with Pseudomonas aeruginosa PAO1 and Desulfovibrio desulfuricans (ATCC 29577; Medilanski et al, 2003).…”

Section: Microscopic Studiesmentioning

confidence: 99%

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Adhesion ofPseudomonas putidaKT2442 Is Mediated by Surface Polymers at the Nano- and Microscale

Bell

Arora

Camesano

2005

Environmental Engineering Science

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Pseudomonas putida KT2442 is a bacterium with potential for use in bioremediation of chlorinated hydrocarbons in soils, and its behavior in the subsurface is believed to be controlled by surface polymers. The role of surface macromolecules on the retention and adhesion behavior of this bacterium was examined by bacterial treatment with cellulase, an enzyme that breaks down the ␤(1 Ǟ 4) linkages of cellulose and similar macromolecules. Enzymatic treatment involved centrifugation to separate bacteria from suspending media. Two types of control systems were evaluated, namely, cells separated from growth media via filtration and centrifugation. Bacterial retention was quantified by the collision efficiency, ␣, the fraction of collisions that result in attachment. In batch retention studies (to glass), both controls had the same ␣ values (1.19 Ϯ 0.25, 1.20 Ϯ 0.23, for filtered and centrifuged cells, respectively). In column transport/retention assays (to quartz), ␣s for the control groups were not statistically different from one another (0.34 Ϯ 0.06, 0.45 Ϯ 0.07, for filtered and centrifuged cells, respectively; data fails Mann-Whitney Rank Sum test). Treatment with cellulase decreased cell retention in both systems. The ␣ values were decreased by 40% for cellulase-treated cells in batch tests, to 0.69 Ϯ 0.13, and in column tests, cells treated with cellulase had ␣ values below those from either control group (0.21 Ϯ 0.05). Retention was correlated with nanoscopic adhesion forces measured with an atomic force microscope (AFM), as treatment with cellulase decreased adhesion forces from 1.05 Ϯ 0.07 to 0.51 Ϯ 0.03 nN. These results suggest that surface modification of P. putida KT2442 with cellulase alters adhesion/retention properties at the batch, column, and nanoscale, due to removal of polysaccharide material.

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Section: Bacterial Retention In Batch and Column Assayscontrasting

confidence: 79%

Section: Microscopic Studiesmentioning

confidence: 99%

Adhesion ofPseudomonas putidaKT2442 Is Mediated by Surface Polymers at the Nano- and Microscale

Bell

Arora

Camesano

2005

Environmental Engineering Science

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“…In this study, the cathodic protection categorized as the impressed current method type was applied to an artificial biofilm containing Pseudomonas aeruginosa (PAO1) (Medilanski et al, 2003) as a representative constituent of biofilm on carbon steel coupon. The effect of cathodic protection on bacterial viability in the biofilm was also investigated to clarify the biocidal mechanisms of cathodic protection.…”

Section: Introductionmentioning

confidence: 99%

Biocidal effect of cathodic protection on bacterial viability in biofilm attached to carbon steel

Miyanaga

Terashi

Kawai

et al. 2007

Biotech & Bioengineering

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Biofilm formed on carbon steel by various species of bacterial cells causes serious problems such as corrosion of steel, choking of flow in the pipe, deterioration of the heat-transfer efficiency, and so on. Cathodic protection is known to be a reliable method for protecting carbon steel from corrosion. However, the initial attachment of bacteria to the surface and the effects of cathodic protection on bacterial viability in the biofilm have not been clarified. In this study, cathodic protection was applied to an artificial biofilm containing Pseudomonas aeruginosa (PAO1), a biofilm constituent, on carbon steel. The aims of this study were to evaluate the inhibition effect of cathodic protection on biofilm formation and to reveal the inhibition mechanisms. The viability of PAO1 in artificial biofilm of 5 mm thickness on cathodically protected steel decreased to 1% of the initial cell concentration. Analysis of pH distribution in the artificial biofilm by pH microelectrode revealed that pH in proximity to carbon steel increased to approximately 11 after cathodic protection for 5 h. Moreover, 99% of region in the artificial biofilm was under the pH conditions of over nine. A simulation of pH profile was shown to correspond to experimental values. These results indicate cells in the artificial biofilm were killed or damaged by cathodic protection due to pH increase.

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Potential of the adhesion of bacteria isolated from drinking water to materials

Simões

Oliveira

et al. 2007

J. Basic Microbiol.

117

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Heterotrophic bacteria (11 genera, 14 species, 25 putative strains) were isolated from drinking water, identified either biochemically or by partial 16s rDNA gene sequencing and their adherence characteristics were determined by two methods: i. thermodynamic prediction of adhesion potential by measuring hydrophobicity (contact angle measurements) and ii. by measuring adherence to eight different substrata (ASI 304 and 316 stainless steel, copper, polyvinyl chloride, polypropylene, polyethylene, silicone and glass). All the test organisms were hydrophilic and inter-species variation in hydrophobicity occurred only for Comamonas acidovorans. Stainless steel 304 (SS 304), copper, polypropylene (PP), polyethylene (PE) and silicone thermodynamically favoured adhesion for the majority of test strains (>18/25), whilst adhesion was generally less thermodynamically favorable for stainless steel 316 (SS 316), polyvinyl chloride (PVC) and glass. The predictability of thermodynamic adhesion test methods was validated by comparison with 24-well microtiter plate assays using nine reference strains and three adhesion surfaces (SS 316, PVC and PE). Results for Acinetobacter calcoaceticus, Burkolderia cepacia and Stenotrophomonas maltophilia sp. 2 were congruent between both methods whilst they differed for the other bacteria to at least one material. Only A. calcoaceticus had strongly adherent properties to the three tested surfaces. Strain variation in adhesion ability was detected only for Sphingomonas capsulata. Analysis of adhesion demonstrated that in addition to physicochemical surface properties of bacterium and substratum, biological factors are involved in early adhesion processes, suggesting that reliance on thermodynamic approaches alone may not accurately predict adhesion capacity.

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Mutual Influences ofPseudomonas aeruginosaandDesulfovibrio desulfuricanson their Adhesion to Stainless Steel

Cited by 8 publications

References 18 publications

Adhesion ofPseudomonas putidaKT2442 Is Mediated by Surface Polymers at the Nano- and Microscale

Adhesion ofPseudomonas putidaKT2442 Is Mediated by Surface Polymers at the Nano- and Microscale

Biocidal effect of cathodic protection on bacterial viability in biofilm attached to carbon steel

Potential of the adhesion of bacteria isolated from drinking water to materials

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