Mutual Exclusion of Sensory Bristles and Tendons on the Notum of Dipteran Flies

Usui, Keisuke; Pistillo, Daniela; Simpson, Patricia

doi:10.1016/j.cub.2004.06.026

Cited by 35 publications

(54 citation statements)

References 39 publications

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“…The following strains were used for the study of CG2446 (Amun): eyeless (ey)-Gal4 (Bose et al 2006) (Bloomington Drosophila Stock Center); decapentaplegic (dpp)-Gal4/TM6B (StaehlingHampton et al 1994); pannier (pnr)-Gal4/TM3 (Heitzler et al 1996), a gift from Gines Morata, Centro de Biología Molecular Severo Ochoa (Madrid); patched (ptc)-Gal4 (Speicher et al 1994) (Bloomington Drosophila Stock Center); scabrous (sca)-Gal4/CyO (Mlodzik et al 1990), a gift from Andrea Brand, University of Cambridge (Cambridge, UK); UAS-myr-mRFP/ TM6B (Bloomington Drosophila Stock Center); stripe MD710 (sr)-Gal4/TM6B (Calleja et al 2002;Usui et al 2004), a gift from Pat Simpson, University of Cambridge (Cambridge, UK); UASAmunRNAi and UAS-Dicer2 (Dietzl et al 2007), obtained from the Vienna Drosophila RNAi Center; and P[lArB]A101.IF3 (neur A101 -LacZ)/TM3 (Bellen et al 1989), a gift of Hugo Bellen, Baylor College of Medicine (Houston).…”

Section: Methodsmentioning

confidence: 99%

A Screen for Modifiers of Notch Signaling Uncovers Amun, a Protein With a Critical Role in Sensory Organ Development

et al. 2009

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Notch signaling is an evolutionarily conserved pathway essential for many cell fate specification events during metazoan development. We conducted a large-scale transposon-based screen in the developing Drosophila eye to identify genes involved in Notch signaling. We screened 10,447 transposon lines from the Exelixis collection for modifiers of cell fate alterations caused by overexpression of the Notch ligand Delta and identified 170 distinct modifier lines that may affect up to 274 genes. These include genes known to function in Notch signaling, as well as a large group of characterized and uncharacterized genes that have not been implicated in Notch pathway function. We further analyze a gene that we have named Amun and show that it encodes a protein that localizes to the nucleus and contains a putative DNA glycosylase domain. Genetic and molecular analyses of Amun show that altered levels of Amun function interfere with cell fate specification during eye and sensory organ development. Overexpression of Amun decreases expression of the proneural transcription factor Achaete, and sensory organ loss caused by Amun overexpression can be rescued by coexpression of Achaete. Taken together, our data suggest that Amun acts as a transcriptional regulator that can affect cell fate specification by controlling Achaete levels.

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Section: Methodsmentioning

confidence: 99%

A Screen for Modifiers of Notch Signaling Uncovers Amun, a Protein With a Critical Role in Sensory Organ Development

et al. 2009

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“…Alternatively, developmental constraints could restrict the emergence of macrochaetes to particular sites. Indeed the additional bristles resulting from selection are not located over the sites of attachment of the flight muscles (Usui et al, 2004). There appears to be a constraint in the form of the indirect flight muscles that lie just below the surface of the scutum (Figure 6).…”

Section: Origin Of Stereotyped Patternsmentioning

confidence: 99%

“…These attach to the cuticle through tendons whose precursors are selected from the same epithelium as the bristle precursors (Lee et al, 1995;Fernandes et al, 1996;Frommer et al, 1996;Volk, 1999). In contrast to the bristles, the pattern of flight muscles and their attachment sites is highly conserved throughout the Diptera (Tiegs, 1955;Usui et al, 2004). Remarkably the flight muscles and the leg jump muscle attach in longitudinal domains that are between the rows of macrochaetes (Usui et al, 2004).…”

Section: Origin Of Stereotyped Patternsmentioning

confidence: 99%

“…In contrast to the bristles, the pattern of flight muscles and their attachment sites is highly conserved throughout the Diptera (Tiegs, 1955;Usui et al, 2004). Remarkably the flight muscles and the leg jump muscle attach in longitudinal domains that are between the rows of macrochaetes (Usui et al, 2004). From an examination of several hundred species, none were found in which the macrochaetes were located over the sites of muscle attachment.…”

Section: Origin Of Stereotyped Patternsmentioning

confidence: 99%

“…Bristle precursors develop in areas of ac-sc expression; tendon precursors develop in areas of expression of stripe (sr), a gene encoding a transcription factor with zinc finger motifs (Lee et al, 1995;Frommer et al, 1996). The domains of expression of ac-sc and sr are spatially segregated in Drosophila and Calliphora vicina (Usui et al, 2004;Richardson and Simpson, 2006). Experimentally contrived mis-expression of sr at the sites where bristle precursors develop, results in a loss of bristles in Drosophila; similarly mis-expression of ac-sc at the sites of tendon development interferes with muscle attachment (Usui et al, 2004).…”

Section: Origin Of Stereotyped Patternsmentioning

confidence: 99%

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The origin and evolution of stereotyped patterns of macrochaetes on the nota of cyclorraphous Diptera

Simpson

Marcellini

2006

Heredity

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A long-standing problem in evolutionary biology is how genetic variation arises within populations and evolves to make species anatomically different. Many of the morphological differences in body plans between animal groups are thought to result from changes in gene expression during development. The rules governing the structure and evolution of cis-regulatory gene sequences are unknown, however, and the evolution of traits between closely related species remains relatively unexplored at a molecular level. To study the evolution of gene regulation, it is necessary to find a tractable trait that varies between species and for which the genetic regulation is well known in at least one of the species. The stereotyped, two-dimensional pattern of bristles on the thorax of Drosophila has been intensively investigated and is due to a precise spatial expression of proneural genes. Other species of flies have different bristle patterns and so comparisons between them provide a good paradigm for the study of changes in gene regulation. Here, we review the current state of understanding of these changes.

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Gene expression patterns determine the differential numbers of dorsocentral macrochaetes between Musca domestica and Drosophila melanogaster

Liang

Peng

Sun

et al. 2018

Genesis

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The evolutionary differences in sensory bristle patterns on the thorax of dipterans are an excellent model for studying the patterns of evolutionary development. We observed that Drosophila melanogaster has two pairs of the large bristles, called macrochaetes, in the dorsocentral (DC) region of the notum, while Musca domestica retains six DC macrochaetes. To explore possible mechanism by which these two dipteran species have different numbers of DC bristles, we compared the corresponding protein sequences, the gene expression levels and the spatial expression patterns of five genes (scute, pnr, ush, hairy, and emc) for bristle development between two species. We also checked the overexpression of scute and emc in transgenic flies. The results demonstrated a strong conservation of five protein sequences between these two species. The mRNA expression of the five genes differed significantly between D. melanogaster and M. domestica. The gene expression patterns exhibited a species‐specific pattern during the larval development stage. It suggests that the function of these genes has been conserved in regulating the development of macrocheates between housefly and fruit fly, whereas the gene expression levels, especially spatial expression patterns lead to species‐specificity in DC bristles.

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Mutual Exclusion of Sensory Bristles and Tendons on the Notum of Dipteran Flies

Abstract: The pattern of flight muscles has changed little throughout the Diptera and we argue that the sites of muscle attachment may have constrained the positioning of bristles during the course of evolution. This could account for the pattern of four bristle rows on the scutum.

Cited by 35 publications

References 39 publications

A Screen for Modifiers of Notch Signaling Uncovers Amun, a Protein With a Critical Role in Sensory Organ Development

A Screen for Modifiers of Notch Signaling Uncovers Amun, a Protein With a Critical Role in Sensory Organ Development

The origin and evolution of stereotyped patterns of macrochaetes on the nota of cyclorraphous Diptera

Gene expression patterns determine the differential numbers of dorsocentral macrochaetes between Musca domestica and Drosophila melanogaster

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