Mutual Exclusion of
            <i>Methanobrevibacter</i>
            Species in the Human Gut Microbiota Facilitates Directed Cultivation of a
            <i>Candidatus</i>
            Methanobrevibacter Intestini Representative

Low, Adrian; Lee, Jolie Kar Yi; Gounot, Jean-Sébastien; Ravikrishnan, Aarthi; Ding, Yichen; Saw, Woei-Yuh; Tan, Linda Wei Lin; Moong, Don Kyin Nwe; Teo, Yik Ying; Nagarajan, Niranjan; Seedorf, Henning

doi:10.1128/spectrum.00849-22

Cited by 8 publications

(5 citation statements)

References 48 publications

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“…Upon closer examination of these networks, we found that M. smithii or Ca. M. intestini consistently co-occurred regardless of the age group, which was in contrast to previous findings based on Sanger sequencing (73). Additionally, the family Christensenellaceae was consistently associated with these archaeal species across all age groups.…”

Section: Discussioncontrasting

confidence: 99%

Age-Related Dynamics of Methanogenic Archaea in the Human Gut Microbiome: Implications for Longevity and Health

Mohammadzadeh,

Mahnert,

Shinde

et al. 2024

Preprint

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The reciprocal relationship between aging and alterations in the gut microbiota is a subject of ongoing research. While the role of bacteria in the gut microbiome is well-documented, specific changes in the composition of methanogens during extreme aging and the impact of high methane production in general on health remain unclear. To address these questions, we analyzed metagenomic data from the stool samples of young adults (n=127, Age: 19-59 y), older adults (n=86), and centenarians (n=34, age: 100-109 years). Our findings reveal a compelling link between age and the prevalence of high methanogen phenotype, while overall archaeal diversity diminishes. Surprisingly, the archaeal composition of methanogens in the microbiome of centenarians appears more akin to that of younger adults, showing an increase in Methanobrevibacter smithii, rather than Ca. M. intestini. Remarkably, Ca. M. intestini emerged as a central player in the network stability of adults, paving the way for M. smithii in older adults and centenarians. Notably, centenarians exhibit a highly complex and stable network of these two methanogens with other bacteria. Furthermore, the mutual exclusion between Lachnospiraceae and these methanogens throughout all age groups suggests that these archaeal communities may compensate for the age-related drop in Lachnospiraceae by co-occurring with butyrate-producing Oscillospiraceae. This study underscores the crucial role of the archaeal microbiome in human physiology and aging. It highlights age-related shifts in methanogen composition, emphasizing the significance of Ca. M. intestini and the partnership between methanogens and specific butyrate-producing bacteria for enhanced health and potential longevity.

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Section: Discussioncontrasting

confidence: 99%

Age-Related Dynamics of Methanogenic Archaea in the Human Gut Microbiome: Implications for Longevity and Health

Mohammadzadeh,

Mahnert,

Shinde

et al. 2024

Preprint

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“…Also, there is growing evidence showing the Erysipelatoclostridium plays in immune response, inflammation, and response to cancer immune therapy 44 . Methanobacteriaceae is the predominant methanogen in the human gut, and its presence or absence in the gastrointestinal tract has been linked to a variety of health characteristics, including changes in body weight and cardiovascular disease 45 .…”

Section: Discussionmentioning

confidence: 99%

Associations between gut microbiota and three prostate diseases: a bidirectional two-sample Mendelian randomization study

Liu,

Dong

2024

Sci Rep

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According to previous observational researches and clinical trials, the gut microbiota is related to prostate diseases. However, the potential association between gut microbiota and prostate disorders is still uncertain. We first identified groups of gut microbiota based on the phylum, class, order, family, and genus levels from consortium MiBioGen. And we acquired prostate diseases statistics from the FINNGEN study and PRACTICAL consortium. Next, two-sample Mendelian randomization was used to investigate the potential associations between three prevalent prostate disease and gut microbiota. In addition, we performed a reverse MR analysis and Benjamini-Hochberg (BH) test for further research. We investigated the connection between 196 gut microbiota and three prevalent prostate diseases. We identified 42 nominally significant associations and 2 robust causative links. Upon correction for multiple comparisons using the Benjamini–Hochberg procedure, our analysis revealed a positive correlation between the risk of prostatitis and the presence of the taxonomic order Gastranaerophilales. Conversely, the risk of prostate cancer exhibited an inverse correlation with the presence of the taxonomic class Alphaproteobacteria. Our study revealed the potential association between gut microbiota and prostate diseases. The results may be useful in providing new insights for further mechanistic and clinical studies of prostate diseases.

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“…7, P > 0.05). For detailed proteomic and metabolomic studies, this study focused on vesicles from Methanobrevibacter species, the most abundant archaea in the human gut microbiome, comprising up to 4% of the microbiome 86 . Methanobrevibacter species rely on syntrophic bacterial partners that provide small organic compounds like H 2 (or formate) and CO 2 for methanogenesis 63,87,88 .…”

Section: Discussionmentioning

confidence: 99%

Proteomic and Metabolomic Profiling of Archaeal Extracellular Vesicles from the Human Gut

Weinberger,

Darnhofer,

Mertelj

et al. 2024

Preprint

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One potential mechanism for microbiome-host, and microbiome constituents’ interaction and communication involves extracellular vesicles (EVs). Here, for the first time, we report the capability of two M. smithii strains (ALI and GRAZ-2), Candidatus M. intestini, and Methanosphaera stadtmanae, as underrepresented components of the gut microbiome, to produce EVs. Interesting, size, morphology, and composition of AEVs were comparable to bacterial EVs, as indicated by ultrastructure, composition, proteomic and metabolomic analyses; however, EVs were substantially less prevalent in the studied Archaea. When looking at the proteomics more precisely, although AEVs from M. smithii ALI and M. intestini were found to be carrying unique proteins (n=135 and n=30, respectively), the shared proteins in AEVs within this genus (n=229), were mostly adhesins(/like) proteins, or proteins with IG-like domains. One remarkable observation was the uptake of AEVs obtained from Methanosphaera stadtmanae and the studied Methanobrevibacter species by human monocytes and the subsequent IL-8 secretion.

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Mutual Exclusion of Methanobrevibacter Species in the Human Gut Microbiota Facilitates Directed Cultivation of a Candidatus Methanobrevibacter Intestini Representative

Abstract: Methanogens are important hydrogen consumers in the gut and are associated with differing host health. Here, we determine the prevalence and abundance of archaeal species in the guts of a multi-ethnic cohort of healthy Singapore residents.

Cited by 8 publications

References 48 publications

Age-Related Dynamics of Methanogenic Archaea in the Human Gut Microbiome: Implications for Longevity and Health

Age-Related Dynamics of Methanogenic Archaea in the Human Gut Microbiome: Implications for Longevity and Health

Associations between gut microbiota and three prostate diseases: a bidirectional two-sample Mendelian randomization study

Proteomic and Metabolomic Profiling of Archaeal Extracellular Vesicles from the Human Gut

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