MutT Homolog1 has multifaceted role in glioma and is under the apparent orchestration by Hypoxia Inducible factor1 alpha

Bhavya, Bharathan; Easwer, H. V.; Vilanilam, George C; Anand, C.R.; Sreelakshmi, K.; Urulangodi, Madhusoodanan; Rajalakshmi, P; Neena, Issac; Padmakrishnan, C.J.; Menon, Girish; Krishnakumar, K.; Deepti, Ayswaria; Gopala, Srinivas

doi:10.1016/j.lfs.2020.118673

Cited by 5 publications

(6 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The patients selected for the study were in the age range of 7-65 years with a median age of 42. Informed written consent was obtained from the patients or relatives prior to tissue collection and processing, which has been previously described (9). For patients who were <18 years of age, consent was obtained by the parents of those patients.…”

Section: Methodsmentioning

confidence: 99%

“…MTH1 belongs to a superfamily of enzymes known as the nucleoside diphosphates linked to moiety-X (NUDIX) hydrolases and is potentially the only enzyme involved in preventing mutations in DNA (8). In a previous study, the authors reported that the silencing of MTH1 affected glioma cell migration and invasion, and inhibited the regulators of angiogenesis (9).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The mutant isocitrate dehydrogenase 1 product, 2‑hydroxyglutarate, activates MutT homolog 1 in glioma cells via the augmentation of reactive oxygen species levels

et al. 2022

Self Cite

View full text Add to dashboard Cite

MutT homolog1 (MTH1) is an enzyme responsible for removing oxidized nucleotides from cells. The activation of MTH1 has been reported in a number of cancer cell types and is considered to be responsible for imparting resistance towards anticancer drugs. While there are several known mechanisms for the activation of MTH1 in cancer cells, the present study aimed to evaluate the role of mutant isocitrate dehydrogenase1 (mIDH1)-mediated reactive oxygen species (ROS) production in the activation of MTH1 in glioma cells. MTH1 was found to be upregulated in both mIDH1-expressing cells and 2-hydroxyglutarate (2-HG)-treated cells. mIDH1 and its product, 2-HG, increased the levels of ROS in cultured glioblastoma cells. Furthermore, the increased expression and activity of MTH1 were observed in glioma tissues harboring mIDH1 compared to tissues with wild-type IDH1. On the whole, the findings of the present study unveil a novel mechanism of activation of MTH1 in glioma cells harboring mutant IDH1.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The mutant isocitrate dehydrogenase 1 product, 2‑hydroxyglutarate, activates MutT homolog 1 in glioma cells via the augmentation of reactive oxygen species levels

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…: SCT/IEC/932/AUGUST-2016). Informed written consents were obtained from the patients or relatives prior to tissue collection and further processing were cited elsewhere (Bhavya et al 2020b).…”

Section: Collection Of Tissue Biopsymentioning

confidence: 99%

“…Glioblastoma cells and glioma tissues were processed for protein expression analysis and probed for desired proteins along with loading controls as described elsewhere (Bhavya et al 2020b). The primary antibodies used were: MTH1 (1:500; Novus Biologicals, Centennial, Colorado, United States), vinculin, βactin (1:1000; Sigma-Aldrich, Gillingham, UK), OGG1 and mIDH1 (1:1000, ImmunoTag, St. Louis, MO, USA), GPx4 and MnSOD (1:1000; Abcam, Cambridge, United Kingdom).…”

Section: Immunoblotmentioning

confidence: 99%

“…MTH1 belongs to a superfamily of enzymes called the nucleoside diphosphates linked to moiety-X (NUDIX) hydrolases and is potentially the only one enzyme involved in preventing mutations in DNA (Gad et al 2014). In our previous study, we had reported that silencing of MTH1 can affect glioma cell migration, invasion and inhibits the regulators of angiogenesis (Bhavya et al 2020b). In the present study, we hypothesized that mutant-IDH1 (mIDH1) and its product, 2-HG, could be responsible for the activation of MTH1, via the production of ROS.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Mutant Isocitrate Dehydrogenase 1 Product, 2-Hydroxy Glutarate, Activates MutT Homolog 1 in Glioma Cells via Augmentation of Reactive Oxygen Species

Bhavya

Anand

Urulangodi

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

MutT Homolog1 (MTH1) is an enzyme responsible for removing oxidized nucleotides from cells. Activation of MTH1 is reported in many cancer cells and is thought to be responsible for imparting resistance towards anticancer drugs. While there are several mechanisms for the activation of MTH1 in cancer cells, this study aimed to evaluate the role of mutant Isocitrate Dehydrogenase1 (mIDH1) - mediated reactive oxygen species (ROS) in the activation of MutT Homolog1 in glioma cells. MTH1 was found to be upregulated in both mIDH1 expressed and 2- HG treated cells. mIDH1 and its product, 2-HG, increased the ROS levels in the cultured glioblastoma cells. Further, increased expression and activity of MTH1 was observed in glioma tissues harboring mIDH1 compared to tissues with wild-type IDH1. Our study unveils a novel mechanism of activation of MTH1 in cells harboring mutant IDH1.

show abstract

Specific 8-oxo-dGTPase activity of MTH1 (NUDT1) protein as a quantitative marker and prognostic factor in human colorectal cancer

Białkowski

Szpila

2021

Free Radical Biology and Medicine

View full text Add to dashboard Cite

MutT Homolog1 has multifaceted role in glioma and is under the apparent orchestration by Hypoxia Inducible factor1 alpha

Cited by 5 publications

References 35 publications

The mutant isocitrate dehydrogenase 1 product, 2‑hydroxyglutarate, activates MutT homolog 1 in glioma cells via the augmentation of reactive oxygen species levels

The mutant isocitrate dehydrogenase 1 product, 2‑hydroxyglutarate, activates MutT homolog 1 in glioma cells via the augmentation of reactive oxygen species levels

Mutant Isocitrate Dehydrogenase 1 Product, 2-Hydroxy Glutarate, Activates MutT Homolog 1 in Glioma Cells via Augmentation of Reactive Oxygen Species

Specific 8-oxo-dGTPase activity of MTH1 (NUDT1) protein as a quantitative marker and prognostic factor in human colorectal cancer

Contact Info

Product

Resources

About