Abstract:The dI component of Rhodospirillum rubrum transhydrogenase has a single Trp residue (Trp 72 ), which has distinctive optical properties, including short-wavelength fluorescence emission with clear vibrational fine structure, and long-lived, wellresolved phosphorescence emission. We have made a set of mutant dI proteins in which residues contacting Trp 72 are conservatively substituted. The room-temperature fluorescence-emission spectra of our three Met 97 mutants are blue shifted by ;4 nm, giving them a shorte… Show more
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