Mutations in the rice liguleless gene result in a complete loss of the auricle, ligule, and laminar joint

Lee, Jinwon; Kim, Song Lim; Yim, Jieun; An, Gynheung

doi:10.1007/s11103-007-9196-1

Cited by 128 publications

(106 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Preferential expression of previously reported genes regulating leaf angle, including LG1 (Lee et al, 2007), BU1 (Tanaka et al, 2009), LPA1 , and CYCU4:1 (Sun et al, 2015), at the lamina joint , which can be divided into six stages (S1-S6). The lamina joint of the eighth leaf was observed by cross section, and the adaxial side (middle; bars = 100 mm) and the abaxial side (right; bars = 100 mm) were enlarged.…”

Section: Cytological Observation Reveals the Distinct Characteristicsmentioning

confidence: 98%

“…Deficiency of lamina joint structure (Lee et al, 2007), suppressed longitudinal elongation of adaxial parenchyma cells, and increased division of abaxial sclerenchyma cells of the lamina joint (Zhang et al, 2009a;Sun et al, 2015) result in leaf erectness. Conversely, increased expansion or proliferation of adaxial parenchyma cells leads to increased leaf inclination (Zhao et al, 2010(Zhao et al, , 2013Zhang et al, 2015).…”

mentioning

confidence: 99%

“…OsLIGULELESS1 (OsLG1) encodes an SBP domain-containing TF, and mutant oslg1 abolishes the ligule, auricle, and lamina joint (Lee et al, 2007). LAX PANICLE (LAX) encodes a basic helix-loop-helix TF the ectopic expression of which caused excessive bending of rice lamina joint (Komatsu et al, 2003).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Dynamic Cytology and Transcriptional Regulation of Rice Lamina Joint Development

2017

View full text Add to dashboard Cite

Rice (Oryza sativa) leaf angle is determined by lamina joint and is an important agricultural trait determining leaf erectness and, hence, the photosynthesis efficiency and grain yield. Genetic studies reveal a complex regulatory network of lamina joint development; however, the morphological changes, cytological transitions, and underlying transcriptional programming remain to be elucidated. A systemic morphological and cytological study reveals a dynamic developmental process and suggests a common but distinct regulation of the lamina joint. Successive and sequential cell division and expansion, cell wall thickening, and programmed cell death at the adaxial or abaxial sides form the cytological basis of the lamina joint, and the increased leaf angle results from the asymmetric cell proliferation and elongation. Analysis of the gene expression profiles at four distinct developmental stages ranging from initiation to senescence showed that genes related to cell division and growth, hormone synthesis and signaling, transcription (transcription factors), and protein phosphorylation (protein kinases) exhibit distinct spatiotemporal patterns during lamina joint development. Phytohormones play crucial roles by promoting cell differentiation and growth at early stages or regulating the maturation and senescence at later stages, which is consistent with the quantitative analysis of hormones at different stages. Further comparison with the gene expression profile of leaf inclination1, a mutant with decreased auxin and increased leaf angle, indicates the coordinated effects of hormones in regulating lamina joint. These results reveal a dynamic cytology of rice lamina joint that is fine-regulated by multiple factors, providing informative clues for illustrating the regulatory mechanisms of leaf angle and plant architecture.

show abstract

Section: Cytological Observation Reveals the Distinct Characteristicsmentioning

confidence: 98%

mentioning

confidence: 99%

See 1 more Smart Citation

Dynamic Cytology and Transcriptional Regulation of Rice Lamina Joint Development

2017

View full text Add to dashboard Cite

show abstract

“…Two genes, liguleless1 and 2 (lg1 and lg2, respectively) are important for the leaf angle in maize and rice (Lee et al, 2007;Moreno et al, 1997;Walsh et al, 1998). lg1 encodes a squamosa promoter-binding protein and lg2 encodes a bZIP transcription factor (Moreno et al, 1997;Walsh et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Gene regulatory interactions at lateral organ boundaries in maize

et al. 2014

View full text Add to dashboard Cite

Maize leaves have distinct tissues that serve specific purposes. The blade tilts back to photosynthesize and the sheath wraps around the stem to provide structural support and protect young leaves. At the junction between blade and sheath are the ligule and auricles, both of which are absent in the recessive liguleless1 (lg1) mutant. Using an antibody against LG1, we reveal LG1 accumulation at the site of ligule formation and in the axil of developing tassel branches. The dominant mutant Wavy auricle in blade1 (Wab1-R) produces ectopic auricle tissue in the blade and increases the domain of LG1 accumulation. We determined that wab1 encodes a TCP transcription factor by positional cloning and revertant analysis. Tassel branches are few and upright in the wab1 revertant tassel and have an increased branch angle in the dominant mutant. wab1 mRNA is expressed at the base of branches in the inflorescence and is necessary for LG1 expression. wab1 is not expressed in leaves, except in the dominant mutant. The domain of wab1 expression in the Wab1-R leaf closely mirrors the accumulation of LG1. Although wab1 is not needed to induce lg1 expression in the leaf, LG1 is needed to counteract the severe phenotype of the dominant Wab1-R mutant. The regulatory interaction of LG1 and WAB1 reveals a link between leaf shape and tassel architecture, and suggests the ligule is a boundary similar to that at the base of lateral organs.

show abstract

“…Overexpression of BRASSINOSTEROID UPREGULATED 1 (BU1), a helix-loop-helix transcription factor involved in BR signaling in rice, results in the enhanced lamina joint bending whereas the RNAi plants with suppressed BU1 expression showed the erected leaf phenotype [20]. Os-LIGULELESS1 (OsLG1) encodes a transcription factor containing an SQUAMOSA promoter binding protein domain, and its deficiency mutant, oslg1, shows defect in the ligule, auricle, and laminar joint [21]. Analysis of the leaf angle mutant large leaf angles (lla), a T-DNA insertion mutant, showed that OsWRKY11, a WRKY transcription factor, also regulates leaf inclination [22].…”

Section: Introductionmentioning

confidence: 99%

Rice leaf inclination2, a VIN3-like protein, regulates leaf angle through modulating cell division of the collar

et al. 2010

View full text Add to dashboard Cite

As an important agronomic trait, inclination of leaves is crucial for crop architecture and grain yields. To understand the molecular mechanism controlling rice leaf angles, one rice leaf inclination2 (lc2, three alleles) mutant was identified and functionally characterized. Compared to wild-type plants, lc2 mutants have enlarged leaf angles due to increased cell division in the adaxial epidermis of lamina joint. The LC2 gene was isolated through positional cloning, and encodes a vernalization insensitive 3-like protein. Complementary expression of LC2 reversed the enlarged leaf angles of lc2 plants, confirming its role in controlling leaf inclination. LC2 is mainly expressed in the lamina joint during leaf development, and particularly, is induced by the phytohormones abscisic acid, gibberellic acid, auxin, and brassinosteroids. LC2 is localized in the nucleus and defects of LC2 result in altered expression of cell division and hormone-responsive genes, indicating an important role of LC2 in regulating leaf inclination and mediating hormone effects.

show abstract

Mutations in the rice liguleless gene result in a complete loss of the auricle, ligule, and laminar joint

Cited by 128 publications

References 48 publications

Dynamic Cytology and Transcriptional Regulation of Rice Lamina Joint Development

Dynamic Cytology and Transcriptional Regulation of Rice Lamina Joint Development

Gene regulatory interactions at lateral organ boundaries in maize

Rice leaf inclination2, a VIN3-like protein, regulates leaf angle through modulating cell division of the collar

Contact Info

Product

Resources

About