Mutations in rpoB and katG genes and the inhA operon in multidrug-resistant Mycobacterium tuberculosis isolates from Zambia

Solo, Eddie Samuneti; Nakajima, Chie; Kaile, Trevor; Bwalya, Precious; Mbulo, Grace; Fukushima, Yukari; Chila, Sylvia; Kapata, Nathan; Shah, Yogendra; Suzuki, Yasuhiko

doi:10.1016/j.jgar.2020.02.026

Cited by 16 publications

(27 citation statements)

References 33 publications

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“…This practice could have led to the undetected emergence and silent spread of drug-resistant TB. In a previous study, it was demonstrated that the increase in MDR-TB in Zambia is not the result of transborder transmission but local emergence and possible spread within Zambia [4,5]. The mismanagement of MDR-TB could further the evolution of resistance to other anti-TB drugs.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Mutations Associated with Streptomycin Resistance in Multidrug-Resistant Mycobacterium tuberculosis in Zambia

et al. 2021

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Streptomycin (STR) is recommended for the management of multidrug-resistant tuberculosis (MDR-TB). Streptomycin resistance-conferring mutation types and frequency are shown to be influenced by genotypes of circulating strains in a population. This study aimed to characterize the mutations in MDR-TB isolates and examine their relationship with the genotypes in Zambia. A total of 138 MDR-TB isolates stored at the University Teaching Hospital Tuberculosis Reference Laboratory in Zambia were analyzed using spoligotyping and sequencing of STR resistance-associated genes. Streptomycin resistance was observed in 65.9% (91/138) of MDR-TB isolates. Mutations in rpsL, rrs, and gidB accounted for 33%, 12.1%, and 49.5%, respectively. Amino acid substitution K43R in rpsL was strongly associated with the CAS1_Kili genotype (p < 0.0001). The combination of three genes could predict 91.2% of STR resistance. Clustering of isolates based on resistance-conferring mutations and spoligotyping was observed. The clustering of isolates suggests that the increase in STR-resistant MDR-TB in Zambia is largely due to the spread of resistant strains from inadequate treatment. Therefore, rapid detection of STR resistance genetically is recommended before its use in MDR-TB treatment in Zambia.

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Section: Introductionmentioning

confidence: 99%

Characterization of Mutations Associated with Streptomycin Resistance in Multidrug-Resistant Mycobacterium tuberculosis in Zambia

et al. 2021

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“…resistant MTB strains that are often resistant even to other drugs (5,21). Furthermore, studies have indicated a high prevalence of MDR-MTB carrying this mutation of katG 315 (AGC > ACC) from some high TB burden countries such as Bangladesh (80%) (22), Russian Federation (93%) (23), South Africa (87%) (24), and Zambia (91%) (25).…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Rapid and Simple Detection of Isoniazid-Resistant <i>Mycobacterium tuberculosis</i> Utilizing a DNA Chromatography-Based Technique

Kodera¹,

Yamaguchi

Fukushima

et al. 2021

Jpn J Infect Dis

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“…Fifty-one isolates grown on Ogawa medium (Kyokuto Pharmaceutical Industrial Co., Ltd., Tokyo, Japan) and identified as M. bovis by a MTC-discrimination multiplex PCR [25] and spoligotyping [26] were collected from cattle and wild lechwe antelope from 2004 to 2009 in Zambia [27]. Twenty [28]. Four of those were confirmed as M. bovis and 16 were M. tuberculosis by Capilia TB (Tauns Laboratories Inc., Shizuoka, Japan) and spoligotyping.…”

Section: Ethics Statementmentioning

confidence: 99%

Development of a loop-mediated isothermal amplification (LAMP) method for specific detection of Mycobacterium bovis

et al. 2021

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Bovine tuberculosis (TB) caused by Mycobacterium bovis is a significant health threat to cattle and a zoonotic threat for humans in many developing countries. Rapid and accurate detection of M. bovis is fundamental for controlling the disease in animals and humans, and for the proper treatment of patients as one of the first-line anti-TB drug, pyrazinamide, is ineffective against M. bovis. Currently, there are no rapid, simplified and low-cost diagnostic methods that can be easily integrated for use in many developing countries. Here, we report the development of a loop-mediated isothermal amplification (LAMP) assay for specific identification of M. bovis by targeting the region of difference 4 (RD4), a 12.7 kb genomic region that is deleted solely in M. bovis. The assay's specificity was evaluated using 139 isolates comprising 65 M. bovis isolates, 40 M. tuberculosis isolates, seven M. tuberculosis complex reference strains, 22 non-tuberculous mycobacteria and five other bacteria. The established LAMP detected only M. bovis isolates as positive and no false positives were observed using the other mycobacteria and non-mycobacteria tested. Our LAMP assay detected as low as 10 copies of M. bovis genomic DNA within 40 minutes. The procedure of LAMP is simple with an incubation at a constant temperature. Results are observed with the naked eye by a color change, and there is no need for expensive equipment. The established LAMP can be used for the detection of M. bovis infections in cattle and humans in resource-limited areas.

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Mutations in rpoB and katG genes and the inhA operon in multidrug-resistant Mycobacterium tuberculosis isolates from Zambia

Cited by 16 publications

References 33 publications

Characterization of Mutations Associated with Streptomycin Resistance in Multidrug-Resistant Mycobacterium tuberculosis in Zambia

Characterization of Mutations Associated with Streptomycin Resistance in Multidrug-Resistant Mycobacterium tuberculosis in Zambia

Rapid and Simple Detection of Isoniazid-Resistant <i>Mycobacterium tuberculosis</i> Utilizing a DNA Chromatography-Based Technique

Development of a loop-mediated isothermal amplification (LAMP) method for specific detection of Mycobacterium bovis

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