Mutations in AcrR and RNA Polymerase Confer High-Level Resistance to Psoralen-UVA Irradiation

Worley, Travis K; Weber, Emma A; Acott, Jedidiah D; Shimpi, Rahul; Cole, Jessica; Courcelle, Charmain T.; Courcelle, Justin

doi:10.1128/jb.00126-23

Cited by 1 publication

(1 citation statement)

References 79 publications

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“…NfxB belongs to the TFRs ( 21 ), which have been widely associated with bacterial physiology aspects ( 26 ). In clinical isolates, TFR mutations lead to the de-repression of their targets ( 27 ), some of which are efflux transporters, thus contributing to AMR and adjusting to changing environments ( 28 ). NfxB mutants are commonly found in ciprofloxacin-resistant P. aeruginosa clinical strains ( 29 , 30 ).…”

Section: Discussionmentioning

confidence: 99%

Promoter regulatory mode evolution enhances the high multidrug resistance of tmexCD1-toprJ1

Wang,

Yang,

et al. 2024

mBio

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Antibiotic resistance could rapidly emerge from acquiring the mobile antibiotic resistance genes, which are commonly evolved from an intrinsic gene. The emergence of the plasmid-borne mobilized efflux pump gene cluster tmexCD1-toprJ1 renders the last-resort antibiotic tigecycline ineffective, although its evolutionary mechanism remains unclear. In this study, we investigate the regulatory mechanisms of the progenitor NfxB-MexCD-OprJ, a chromosomally encoded operon that does not mediate antibiotic resistance in the wild-type version, and its homologs, TNfxB1-TMexCD1-TOprJ1 mediating high-level tigecycline resistance, and TNfxB3-TMexCD3-TOprJ1. Mechanistic studies demonstrated that in nfxB-mexCD-oprJ , MexCD expression was under a weaker promoter, P mexC and inhibited by a strong repressor NfxB. For tmexCD1-toprJ1 , TMexCD1 was highly expressed owing to the presence of a strong promoter, P tmexC1 , and an inactive suppressor, TNfxB1, with a T39R mutation that rendered it unable to bind to promoter DNA. In tnfxB3-tmexCD3-toprJ1b , TMexCD3 expression was intermediate because of the local regulator TNfxB3, which binds to two inverted repeat sequences of P tmexC . Additionally, TNfxB3 exhibited lower protein expression and weaker DNA binding affinity than its ancestor NfxB, together with their promoter activities difference explaining the different expression levels of tmexCD-toprJ homologs. Distinct fitness burdens on these homologs-carrying bacteria were observed due to the corresponding expression level, which might be associated with their global prevalence. In summary, our data depict the mechanisms underlying the evolution and dissemination of an important mobile antibiotic resistance gene from an intrinsic chromosomal gene. IMPORTANCE As antibiotic resistance seriously challenges global health, tigecycline is one of the few effective drugs in the pipeline against infections caused by multidrug-resistant pathogens. Our previous work identified a novel tigecycline resistance efflux pump gene cluster tmexCD1-toprJ1 in animals and humans, together with its various variants, a rising clinical concern. Herein, this study focused on how the local regulation modes of tmexCD1-toprJ1 evolved to a highly expressed efflux pump. Through comparative analysis between three tnfxB-tmexCD-toprJ homologs and their progenitor nfxB-mexCD-oprJ , modes, we demonstrated the evolutionary dynamics from a chromosomal silent gene to an active state. We found the de-repression of the local regulator and an increase of the promoter activity work together to promote a high production of drug efflux machines and enhance multidrug resistance. Our findings revealed that TMexCD1-TOprJ1 adopts a distinct evolutionary path to achieve higher multidrug resistance, urgently needing tight surveillance.

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Section: Discussionmentioning

confidence: 99%

Promoter regulatory mode evolution enhances the high multidrug resistance of tmexCD1-toprJ1

Wang,

Yang,

et al. 2024

mBio

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Mutations in AcrR and RNA Polymerase Confer High-Level Resistance to Psoralen-UVA Irradiation

Abstract: Psoralen DNA interstrand cross-links are highly toxic lesions with antimicrobial and anticancer properties. Despite the lack of effective mechanisms for repair, cells can become resistant to cross-linking agents through mechanisms that remain poorly defined.

Cited by 1 publication

References 79 publications

Promoter regulatory mode evolution enhances the high multidrug resistance of tmexCD1-toprJ1

Promoter regulatory mode evolution enhances the high multidrug resistance of tmexCD1-toprJ1

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