Mutations in Acetylcholinesterase2 (ace2) increase the insensitivity of acetylcholinesterase to fosthiazate in the root-knot nematode Meloidogyne incognita

Huang, Wenkun; Wu, Qin-Song; Peng, Huan; Kong, Lingan; Liu, Shiming; Yin, Huaqun; Cui, Ruqiang; Zhan, Linlin; Cui, Jiangkuan; Peng, Deliang

doi:10.1038/srep38102

Cited by 21 publications

(13 citation statements)

References 45 publications

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“…At present, chemical control is the main control measure, but many nematicides are banned because of safety, ecological, and environmental concerns [3]. However, there are many shortcomings in the limited control categories, such as fosthiazate, which can lead to high toxicity to non-target organisms [4] and drug resistance [5,6] and avermectin, which has difficulty to stay structurally unstable [7] and poor mobility in soil. Therefore, it is critical to develop novel types of eco-friendly and highly efficient nematicides.…”

Section: Introductionmentioning

confidence: 99%

Chitosan Oligosaccharide Fluorinated Derivative Control Root-Knot Nematode (Meloidogyne incognita) Disease Based on the Multi-Efficacy Strategy

et al. 2020

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Plant root-knot nematode disease is a great agricultural problem and commercially available nematicides have the disadvantages of high toxicity and limited usage; thus, it is urgent to develop new nematicides derived from nature substances. In this study, a novel fluorinated derivative was synthesized by modifying chitosan oligosaccharide (COS) using the strategy of multiple functions. The derivatives were characterized by FTIR, NMR, elemental analysis, and TG/DTG. The activity assays show that the derivatives can effectively kill the second instar larvae of Meloidogyne incognita in vitro, among them, chitosan-thiadiazole-trifluorobutene (COSSZFB) perform high eggs hatching inhibitory activity. The derivatives can regulate plant growth (photosynthetic pigment), improve immunity (chitinase and β-1,3-glucanase), and show low cytotoxicity and phytotoxicity. According to the multi-functional activity, the derivatives exhibit a good control effect on plant root-knot nematode disease in vivo. The results demonstrate that the COS derivatives (especially fluorinated derivative) perform multiple activities and show the potential to be further evaluated as nematicides.

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Section: Introductionmentioning

confidence: 99%

Chitosan Oligosaccharide Fluorinated Derivative Control Root-Knot Nematode (Meloidogyne incognita) Disease Based on the Multi-Efficacy Strategy

et al. 2020

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“…Resistance to benzimidazoles in the animal parasitic nematode Haemonchus contortus is the result of point mutations (F200Y with F167Y or E198A) within β-tubulin 19 . The presence of 18 point mutations in AChE2 has also been shown to be associated with the fosthiazate resistance in the plant-parasitic nematode, M. incognita 20 .…”

Section: Introductionmentioning

confidence: 99%

The ACE genes in Aphelenchoides besseyi isolates and their expression correlation to the fenamiphos treatment

Hsu

Weng

Chen

et al. 2022

Sci Rep

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Aphelenchoides besseyi could cause great yield losses of rice and many economically important crops. Acetylcholinesterase (AChE) inhibitors were commonly used to manage plant-parasitic nematodes. However, nematodes resistant to AChE inhibitors have been increasingly reported due to the extensive use of these chemicals. The current study was aimed to establish the correlation between fenamiphos (an AChE-inhibitor) sensitivities and acetylcholinesterase genes (ace) by analyzing two isolates of A. besseyi (designated Rl and HSF), which displayed differential sensitivities to fenamiphos. The concentrations of fenamiphos that led to the death of 50% (LD50) of Rl and HSF were 572.2 ppm and 129.4 ppm, respectively. Three ace genes were cloned from A. besseyi and sequenced. Sequence searching and phylogenic analyses revealed that AChEs of R1 and HSF shared strong similarities with those of various vertebrate and invertebrate species. Molecular docking analysis indicated that AChEs-HSF had much higher affinities to fenamiphos than AChEs-R1. Quantitative reverse transcriptase-PCR analyses revealed that expression of three ace genes were downregulated in HSF but were upregulated in Rl after exposure to 100 ppm fenamiphos for 12 h. The results indicated that the expression of the ace genes was modulated in response to fenamiphos in different nematode strains. An increased expression of the ace genes might contribute to fenamiphos-insensitivity as seen in the Rl isolate.

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“…Animal parasitic nematode Haemonchus contortus displaying resistance to benzimidazoles has also been proved to be the result of point mutations on the target site protein β-tubulin (F200Y with F167Y or E198A) 19 . The plant-parasitic nematode, M. incognita has been reported that 18 point mutations in AChE2 are associated with the fosthiazate resistance 20 .…”

Section: Introductionmentioning

confidence: 99%

The ACE Genes in Aphelenchoides Besseyi Isolates and Their Expression Correlation to the Fenamiphos Treatment

Weng

et al. 2021

Preprint

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Aphelenchoides besseyi could cause great yield loss on rice and many economically important crops. Acetylcholinesterase inhibitors were commonly used to mitigate plant parasitic nematodes. However, increasing nematicide-resistance has been reported due to the extensive use of these chemicals. The correlation between the AChE-inhibitor (fenamiphos) sensitivities and acetylcholinesterase (ace) genes in two isolates of A. besseyi (designated Rl and HSF) was established. The LD50 of fenamiphos to Rl and HSF were 572.2 ppm and 129.4 ppm, respectively, indicating that two nematode isolates had different sensitivities to fenamiphos. Three ace genes were cloned and sequenced in A. besseyi, and their homology was supported by phylogenic analysis with AChEs protein sequences from various vertebrate and invertebrate species. Molecular docking showed that the affinities of each AChEs to fenamiphos were higher in HSF isolate, indicating that there should be point mutations in Rl isolate AChEs. Treating the two isolates with 100 ppm fenamiphos for 12 h, three ace genes of HSF isolate were down-regulated but were up-regulated in Rl isolate. The results suggest that fenamiphos can transcriptionally modulate the expression of ace genes, as well as the variants in AChEs and increased expression of ace genes might be associated with fenamiphos-insensitivity in Rl isolate.

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Mutations in Acetylcholinesterase2 (ace2) increase the insensitivity of acetylcholinesterase to fosthiazate in the root-knot nematode Meloidogyne incognita

Cited by 21 publications

References 45 publications

Chitosan Oligosaccharide Fluorinated Derivative Control Root-Knot Nematode (Meloidogyne incognita) Disease Based on the Multi-Efficacy Strategy

Chitosan Oligosaccharide Fluorinated Derivative Control Root-Knot Nematode (Meloidogyne incognita) Disease Based on the Multi-Efficacy Strategy

The ACE genes in Aphelenchoides besseyi isolates and their expression correlation to the fenamiphos treatment

The ACE Genes in Aphelenchoides Besseyi Isolates and Their Expression Correlation to the Fenamiphos Treatment

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