Mutations altering the specificity of the sensor RNA encoded by the Bacillus subtilis pbuE gene

Lobanov, K. V.; Королькова, Н. В.; Eremina, Svetlana; Lopes, Liubov Errais; Proshkin, Sergey; Mironov, A. S.

doi:10.1134/s1022795407060178

Cited by 2 publications

(1 citation statement)

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“…The deduced RNA sequences differ from previously used truncated versions and new numbering nomenclature taking into account these variations were thus employed ( Figure 1A and 1B ). The newly determined transcription start site of the pbuE riboswitch differs from a previous report, which may result from different cellular genetic background [29] .…”

Section: Resultscontrasting

confidence: 72%

Comparative Study between Transcriptionally- and Translationally-Acting Adenine Riboswitches Reveals Key Differences in Riboswitch Regulatory Mechanisms

et al. 2011

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Many bacterial mRNAs are regulated at the transcriptional or translational level by ligand-binding elements called riboswitches. Although they both bind adenine, the adenine riboswitches of Bacillus subtilis and Vibrio vulnificus differ by controlling transcription and translation, respectively. Here, we demonstrate that, beyond the obvious difference in transcriptional and translational modulation, both adenine riboswitches exhibit different ligand binding properties and appear to operate under different regulation regimes (kinetic versus thermodynamic). While the B. subtilis pbuE riboswitch fully depends on co-transcriptional binding of adenine to function, the V. vulnificus add riboswitch can bind to adenine after transcription is completed and still perform translation regulation. Further investigation demonstrates that the rate of transcription is critical for the B. subtilis pbuE riboswitch to perform efficiently, which is in agreement with a co-transcriptional regulation. Our results suggest that the nature of gene regulation control, that is transcription or translation, may have a high importance in riboswitch regulatory mechanisms.

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Section: Resultscontrasting

confidence: 72%