Mutations affecting the pattern of the PNS in drosophila reveal novel aspects of neuronal development

Salzberg, Adi; D'Evelyn, Diana; Schulze, Karen L.; Lee, Ja-Kyeong; Strumpf, D.; Tsai, Linus; Bellen, Hugo J.

doi:10.1016/0896-6273(94)90346-8

Cited by 130 publications

(119 citation statements)

References 62 publications

Supporting

Mentioning

112

Contrasting

Order By: Relevance

“…Interestingly, mutations in pbl have been discovered in a screen for genes required for the development of the peripheral nervous system (Salzberg et al, 1994). These mutants affected the correct migration of the axons in the PNS without obvious defects in cytokinesis.…”

Section: A Novel Function For Pbl In Cell Migrationmentioning

confidence: 99%

The RhoGEF Pebble is required for cell shape changes during cell migration triggered by theDrosophilaFGF receptor Heartless

et al. 2004

View full text Add to dashboard Cite

The FGF receptor Heartless (HTL) is required for mesodermal cell migration in the Drosophila gastrula. We show that mesoderm cells undergo different phases of specific cell shape changes during mesoderm migration. During the migratory phase, the cells adhere to the basal surface of the ectoderm and exhibit extensive protrusive activity. HTL is required for the protrusive activity of the mesoderm cells. Moreover, the early phenotype of htl mutants suggests that HTL is required for the adhesion of mesoderm cells to the ectoderm.In a genetic screen we identified pebble (pbl) as a novel gene required for mesoderm migration. pbl encodes a guanyl nucleotide exchange factor (GEF) for RHO1 and is known as an essential regulator of cytokinesis. We show that the function of PBL in cell migration is independent of the function of PBL in cytokinesis. Although RHO1 acts as a substrate for PBL in cytokinesis, compromising RHO1 function in the mesoderm does not block cell migration. These data suggest that the function of PBL in cell migration might be mediated through a pathway distinct from RHO1. This idea is supported by allele-specific differences in the expressivity of the cytokinesis and cell migration phenotypes of different pbl mutants. We show that PBL is autonomously required in the mesoderm for cell migration. Like HTL, PBL is required for early cell shape changes during mesoderm migration. Expression of a constitutively active form of HTL is unable to rescue the early cellular defects in pbl mutants, suggesting that PBL is required for the ability of HTL to trigger these cell shape changes. These results provide evidence for a novel function of the Rho-GEF PBL in HTL-dependent mesodermal cell migration.

show abstract

Section: A Novel Function For Pbl In Cell Migrationmentioning

confidence: 99%

The RhoGEF Pebble is required for cell shape changes during cell migration triggered by theDrosophilaFGF receptor Heartless

et al. 2004

View full text Add to dashboard Cite

show abstract

“…Among the other 20 transcription factors up-regulated during ectopic eye formation, eight have been described to be involved in other developmental processes. For instance, the roles of lola (12), sequoia (seq) (31), and stich1 (32,33) in embryonic nervous system development were investigated on the basis on their loss-of-function phenotypes. Similarly, loss-of-function mutations in the net gene, which encodes the Drosophila homolog of MATH6, have been described to affect wing vein patterning (34).…”

Section: Genes Detected By Both Arrays As Expressed In the Eye Discs mentioning

confidence: 99%

Analysis of the eye developmental pathway in Drosophila using DNA microarrays

Michaut

Flister

Neeb

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Pax-6 genes encode evolutionarily conserved transcription factors capable of activating the gene-expression program required to build an eye. When ectopically expressed in Drosophila imaginal discs, Pax-6 genes induce the eye formation on the corresponding appendages of the adult fly. We used two different Drosophila full-genome DNA microarrays to compare gene expression in wild-type leg discs versus leg discs where eyeless, one of the two Drosophila Pax-6 genes, was ectopically expressed. We validated these data by analyzing the endogenous expression of selected genes in eye discs and identified 371 genes that are expressed in the eye imaginal discs and up-regulated when an eye morphogenetic field is ectopically induced in the leg discs. These genes mainly encode transcription factors involved in photoreceptor specification, signal transducers, cell adhesion molecules, and proteins involved in cell division. As expected, genes already known to act downstream of eyeless during eye development were identified, together with a group of genes that were not yet associated with eye formation.

show abstract

“…The Drosophila Meis homolog, homothorax (hth), cooperates with extradenticle (Exd), a Drosophila Pbx homolog, to control antenna determination (Casares and Mann, 1998), patterning of the embryonic¯y peripheral nervous system (Salzberg et al, 1994;Kurant et al, 1998), and the suppression of eye development . The interaction between Hth and Exd triggers the nuclear localization of Exd, thus allowing for proper function of the protein complex Kurant et al, 1998;Rieckhof et al, 1997;Abu-Shaar et al, 1999;Berthelsen et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Xmeis1, a protooncogene involved in specifying neural crest cell fate in Xenopus embryos

et al. 2001

View full text Add to dashboard Cite

Mutations affecting the pattern of the PNS in drosophila reveal novel aspects of neuronal development

Cited by 130 publications

References 62 publications

The RhoGEF Pebble is required for cell shape changes during cell migration triggered by theDrosophilaFGF receptor Heartless

The RhoGEF Pebble is required for cell shape changes during cell migration triggered by theDrosophilaFGF receptor Heartless

Analysis of the eye developmental pathway in Drosophila using DNA microarrays

Xmeis1, a protooncogene involved in specifying neural crest cell fate in Xenopus embryos

Contact Info

Product

Resources

About