Mutations adjacent to the dimple of the canine parvovirus capsid structure affect sialic acid binding

Barbis, Dina P.; Chang, Shwu Fen; Parrish, Colin R.

doi:10.1016/0042-6822(92)90192-r

Cited by 60 publications

(62 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Strain 40/07-1 showed the change R377K that was due to a first-base substitution. This change has been associated with the inability to bind erythrocytes in a non-haemaglutinating CPV mutant, as a consequence of the shortness of the 377-K side chain or different pH with respect to R (Barbis et al, 1992).…”

Section: Discussionmentioning

confidence: 99%

Genetic analysis of feline panleukopenia viruses from cats with gastroenteritis

Decaro¹,

Desario²,

Miccolupo

et al. 2008

Journal of General Virology

View full text Add to dashboard Cite

Thirty-nine parvovirus strains contained in faecal samples collected in Italy (n534) and UK (n55) from cats with feline panleukopenia were characterized at the molecular level. All viruses were proven to be true feline panleukopenia virus (FPLV) strains by a minor groove binder probe assay, which is able to discriminate between FPLV and the closely related canine parvovirus type 2. By using sequence analysis of the VP2 gene, it was found that the FPLV strains detected in Italy and UK were highly related to each other, with a nucleotide identity of 99.1-100 and 99.4-99.8 % among Italian and British strains, respectively, whereas the similarities between all the sequences analysed were 98.6-100 %. Eighty-eight variable positions were detected in the VP2 gene of the field and reference FPLV strains, most of which were singletons. Synonymous substitutions (n557) predominated over non-synonymous substitutions (n531), and the ratio between synonymous and non-synonymous substitutions (dN/dS) was 0.10, thus confirming that evolution of FPLV is driven by random genetic drift rather than by positive selection pressure. Some amino acid mutations in the VP2 protein affected sites that are thought to be responsible for antigenic and biological properties of the virus, but no clear patterns of segregation and genetic markers, were identified, confirming that FPLV is in evolutionary stasis.

show abstract

Section: Discussionmentioning

confidence: 99%

Genetic analysis of feline panleukopenia viruses from cats with gastroenteritis

Decaro¹,

Desario²,

Miccolupo

et al. 2008

Journal of General Virology

View full text Add to dashboard Cite

show abstract

“…Interestingly, not all parvoviruses that require sialic acid for hemagglutination also require sialic acid for binding and infection of host cells (39,41). For instance, canine parvovirus requires sialic acid to hemagglutinate, but it does not require sialic acid to infect cells.…”

Section: Receptor-mediated Binding Of Aav5-tomentioning

confidence: 99%

Binding of Adeno-associated Virus Type 5 to 2,3-Linked Sialic Acid Is Required for Gene Transfer

Walters

Keshavjee

et al. 2001

Journal of Biological Chemistry

315

223

View full text Add to dashboard Cite

Recombinant adeno-associated viruses (AAV) are promising gene therapy vectors. Whereas AAV serotype 2-mediated gene transfer to muscle has partially replaced factor IX deficiency in hemophilia patients, its ability to mediate gene transfer to the lungs for cystic fibrosis is hindered by lack of apical receptors. However, AAV serotype 5 infects human airway epithelia from the lumenal surface. We found that in contrast to AAV2, the apical membrane of airway epithelia contains abundant high affinity receptors for AAV5. Binding and gene transfer with AAV5 was abolished by genetic or enzymatic removal of sialic acid from the cell surface. Furthermore, binding and gene transfer to airway epithelia was competed by lectins that specifically bind 2,3-linked sialic acid. These observations suggest that 2,3-linked sialic acid is either a receptor for AAV5 or it is a necessary component of a receptor complex. Further elucidation of the receptor for this virus should enhance understanding of parvovirus biology and expand the therapeutic targets for AAV vectors.

show abstract

“…These plasmids, which express both VP1 and VP2, are replicationdefective owing to deletion of one or both terminal palindromes and " 0n8-1n0 kb of the NS protein coding region. pC-VP was derived from the CPV non-haemagglutinating mutant BI307 (Parrish et al, 1988 ;Barbis et al, 1992). Plasmids expressing VP1 (pC-VP1 and pF-VP1) were constructed by PCR site-directed mutagenesis (Innis et al, 1990) …”

mentioning

confidence: 99%

Tropic determinant for canine parvovirus and feline panleukopenia virus functions through the capsid protein VP2.

Spitzer

Parrish

Maxwell

1997

Journal of General Virology

View full text Add to dashboard Cite

Canine parvovirus (CPV) can productively infect canine and feline cell lines whereas feline panleukopenia virus (FPV) is restricted to the latter. The major determinants of tropism are two amino acids in the sequence shared by the capsid proteins, VP1 and VP2. We have shown that a rodent parvovirus-derived transducing genome, containing the luciferase reporter, can be packaged by VP1 and VP2 from separate helper sources. Canine A72 cells and feline CFK cells were transduced with recombinant virions generated using VP1 and VP2 combinations from CPV and FPV. Both VP1 and VP2 were necessary for production of transducing virions. Efficient transduction of A72 cells required VP2 of CPV. Therefore, the capsid determinants of tropism for CPV and FPV are in VP2, although a source of VP1 is also necessary to produce infectious particles. The results extend similar observations on the tropic determinants of different strains of minute virus of mice.The feline group of autonomous parvoviruses includes feline panleukopenia virus (FPV) and canine parvovirus (CPV), which have greater than 98 % sequence identity but a distinct host range (Truyen et al., 1992). In cell culture, CPV productively infects both canine and feline cell lines whereas FPV is restricted to feline cells. Studies with CPV-FPV recombinant genomes have shown that the canine host range is determined by the sequence between map units 59 and 73, within the overlapping coding sequence for VP1 and VP2 (Parrish & Carmichael, 1986). Site-directed mutagenesis has implicated amino acids 93 and 323 (numbered from the N terminus of VP2) as the major determinants of host range .We previously described the production of transducing recombinant virions derived from the rodent parvovirus LuIII, Author for correspondence : Ian Maxwell.Fax j1 303 315 8272. e-mail Ian.Maxwell!uchsc.edu with substitution of reporter genes for the viral coding sequences (Maxwell et al., 1993 a). The recombinant genome could be packaged by capsid proteins either from LuIII or from related parvoviruses, including CPV or FPV (Maxwell et al., 1993 b ;Spitzer et al., 1996). In particular, packaging of the recombinant with capsid proteins supplied by a CPV helper, but not by a FPV helper, conferred the ability to transduce canine A72 cells efficiently (Spitzer et al., 1996). By packaging a heterologous genome the results confirmed that the tropic determinants for CPV and FPV were specified by the capsid and direct involvement of the encoding DNA was excluded.The capsids of CPV and FPV are composed of 60 capsomers, which consist of VP1 (" 15 % of the mass of the capsid) and VP2 (Tsao et al., 1991 ; Agbandje et al., 1993). These proteins are translated from alternately spliced transcripts from the P38 promoter. However, since the amino acid residues that determine tropism are present in both VP1 and VP2, this function might be mediated by either protein.Knowledge of which protein is involved would help to clarify their roles in establishing infection. Here, we have investigated this question using th...

show abstract

Mutations adjacent to the dimple of the canine parvovirus capsid structure affect sialic acid binding

Cited by 60 publications

References 41 publications

Genetic analysis of feline panleukopenia viruses from cats with gastroenteritis

Genetic analysis of feline panleukopenia viruses from cats with gastroenteritis

Binding of Adeno-associated Virus Type 5 to 2,3-Linked Sialic Acid Is Required for Gene Transfer

Tropic determinant for canine parvovirus and feline panleukopenia virus functions through the capsid protein VP2.

Contact Info

Product

Resources

About