Mutational analysis of the rhodopsin gene in Chinese ADRP families by conformation sensitive gel electrophoresis

Intravitreous Delivery of the Corticosteroid Fluocinolone Acetonide Attenuates Retinal Degeneration in S334ter-4 Rats

“…Rhodopsin gene mutations are prevalent in autosomal dominant RP 45,46 in humans. A high degree of similarity between the transgenic S334ter-4 rat and human RP genotypes and phenotypes makes the S334ter rat a valuable model in examining the pathogenesis of RP and in testing possible treatments.…”

Rhodopsin mutations are scarcely implicated in autosomal recessive retinitis pigmentosa: A preliminary study of Egyptian retinitis pigmentosa patients

“…Exons of the RHO gene were amplified for each study subject using 5 primer pairs (primer sequences are listed in Table 1) [8]. PCR was performed in a reaction volume of 25 ll containing 1 U Taq polymerase, 0.2 ll of each dNTP, 2.5 ll of PCR buffer and 0.4 pmol/ll of each primer.…”

A novel nonsense mutation in Rhodopsin gene in two Indonesian Families with Autosomal Recessive Retinitis Pigmentosa

“…7 PCR was performed in 20µl reaction volumes containing 20ng DNA using the QIAGEN ® Multiplex PCR Kit (QIAGEN, GmbH Hilden, Germany), and in an automated thermo-cycler (GeneAmp, PCR system 9700, Applied Biosystems, Foster City CA, USA). The PCR conditions were an initial denaturation at 95°C for 15 min, followed by 94°C for 45 s, 60°C for 90 s and 72°C for 60 s for 35 cycles, and a final extension at 72°C for 10 min.…”