volume 66, issue 7, P4258-4264 1992
DOI: 10.1128/jvi.66.7.4258-4264.1992
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R H Felkner, M J Roth

Abstract: The role of the N-linked glycosylation sites in the major envelope glycoprotein, SU (gp7O), of Moloney murine leukemia virus has been examined. By using site-specific oligonucleotide-directed mutagenesis, each of the seven glycan addition sites has been individually eliminated. Mutations resulting in the loss of a single glycosylation site produced, intracellularly, stable precursor SU-TM proteins which were 4 to 5 kDa smaller than the wild-type virus SU-TM protein. Mutant A1,4,7, a trimutant lacking three N-l…

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