Mutational analysis of the capsid protein of Leishmania RNA virus LRV1-4

Cadd, Tamarra L.; MacBeth, Kyle J.; Furlong, Dierdre

doi:10.1128/jvi.68.12.7738-7745.1994

Cited by 7 publications

(8 citation statements)

References 23 publications

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“…The finding that the CP N-terminal deletion CP-N71 which lacked 71 amino acid residues was incapable of portant in the packaging of the RNA-dependent RNA polymerase (RDRP) and viral genome into Hv190SV cap-assembly was not surprising in view of the results of a similar study with the totivirus LRV1-4 (Cadd et al, 1994). sids as has been reported for some other viruses (Lazinski and Taylor, 1993).…”

Section: Assembly Of Expressed P78 and P88 Into Virus-like Particlesmentioning

confidence: 57%

Expression, Assembly, and Proteolytic Processing of Helminthosporium victoriae 190S Totivirus Capsid Protein in Insect Cells

et al. 1997

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The dsRNA genome (5.2 kbp) of Helminthosporium victoriae 190S totivirus (Hv190SV) consists of two large overlapping open reading frames (ORFs). The 5' proximal ORF codes for the capsid protein (CP) and the 3' ORF codes for an RNA-dependent RNA polymerase. Although the capsid of Hv190SV is encoded by a single gene, it is composed of two major closely related polypeptides, either p88 and p83 or p88 and p78. Whereas p88 and p83 are phosphoproteins, p78 is nonphosphorylated. Expression of the CP ORF in insect cells generated both p78 and p88 which assembled into virus-like particles. The finding that p78, p83, and p88 share a common N-terminal amino acid sequence is consistent with the determination that N-terminal, but not C-terminal, CP deletions were incompetent for assembly. Evidence was obtained that p78 is derived from p88 via proteolytic cleavage at the C-terminus. Proteolytic processing may play a regulatory role in the virus life cycle since it leads to dephosphorylation of CP and a subsequent decrease in virion transcriptional activity.

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Section: Assembly Of Expressed P78 and P88 Into Virus-like Particlesmentioning

confidence: 57%

Expression, Assembly, and Proteolytic Processing of Helminthosporium victoriae 190S Totivirus Capsid Protein in Insect Cells

et al. 1997

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“…Both LR1 and LR2 were thought to contain single-stranded DNA [ 8 ], but soon after, they were demonstrated to contain circular double-stranded RNAs (dsRNAs) and were renamed Leishmania RNA virus (LRV) [ 9 ]. In the following years, LRV was described in 12 isolates of L. braziliensis and L. guyanensis from the Amazon region [ 10 ], and LRV was identified in one Leishmania ( Leishmania ) major isolate from a human patient in the former Soviet Union [ 11 ]. To the best of our knowledge, there are no studies that have searched for viruses in L .…”

Section: Introductionmentioning

confidence: 99%

The Maze Pathway of Coevolution: A Critical Review over the Leishmania and Its Endosymbiotic History

Cantanhêde

Mata-Somarribas

Chourabi

et al. 2021

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The description of the genus Leishmania as the causative agent of leishmaniasis occurred in the modern age. However, evolutionary studies suggest that the origin of Leishmania can be traced back to the Mesozoic era. Subsequently, during its evolutionary process, it achieved worldwide dispersion predating the breakup of the Gondwana supercontinent. It is assumed that this parasite evolved from monoxenic Trypanosomatidae. Phylogenetic studies locate dixenous Leishmania in a well-supported clade, in the recently named subfamily Leishmaniinae, which also includes monoxenous trypanosomatids. Virus-like particles have been reported in many species of this family. To date, several Leishmania species have been reported to be infected by Leishmania RNA virus (LRV) and Leishbunyavirus (LBV). Since the first descriptions of LRVs decades ago, differences in their genomic structures have been highlighted, leading to the designation of LRV1 in L. (Viannia) species and LRV2 in L. (Leishmania) species. There are strong indications that viruses that infect Leishmania spp. have the ability to enhance parasitic survival in humans as well as in experimental infections, through highly complex and specialized mechanisms. Phylogenetic analyses of these viruses have shown that their genomic differences correlate with the parasite species infected, suggesting a coevolutionary process. Herein, we will explore what has been described in the literature regarding the relationship between Leishmania and endosymbiotic Leishmania viruses and what is known about this association that could contribute to discussions about the worldwide dispersion of Leishmania.

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“…Viruses found in some strains of the protozoan parasite Leishmania consist of spherical capsids containing a dsRNA genome of -5280 nucleotides (nt) (1-3). The two overlapping open reading frames, ORF2 and ORF3, encode the 82 kDa capsid protein (4) and the RNA-dependent RNA polymerase (RDRP) (5), respectively. A comparison of the LRV1 RDRP sequence with homologous sequences from other viruses (5) showed that the yeast L-A/ScV virus RDRP gene is the sequence most similar to LRV.…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic analysis ofLeishmaniaRNA virus andLeishmaniasuggests ancient virus-parasite association

Widmer¹,

Dooley²

1995

Nucl Acids Res

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Some strains of the protozoan parasite Leishmania belonging to the new world species guyanensis and braziliensis are infected with persistent, single-segmented, non-enveloped dsRNA viruses termed LRV1. A single old world strain classified as L. major was recently found to harbor a similar virus, designated LRV2-1. The genomic nucleotide sequences of two LRV1 types (1-1 and 1-4) isolated from two L. guyanensis strains have been determined and found to be highly conserved. In contrast, LRV1-specific cDNA probes derived from the conserved genomic 5' region failed to recognize LRV2 RNA on Northern blots, suggesting a greater degree of divergence between LRV1 and LRV2 than among LRV1 types. This observation suggests a long-term association and coevolution of LRV within each parasite strain. We tested this concept by comparing nucleotide sequences of seven LRV types and PCR fingerprints of the parasite strains from which these viruses were derived. In support of the idea of virus-parasite co-evolution, we find that genetic distances between LRV types mirror the heterogeneity between parasite fingerprints and are clustered according to the geographical origin of the strains. In agreement with the postulated common origin of persistent dsRNA viruses of protozoa and fungi, we conclude that the infection of Leishmania with LRV pre-dates the divergence of Leishmania into different lineages.

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Mutational analysis of the capsid protein of Leishmania RNA virus LRV1-4

Cited by 7 publications

References 23 publications

Expression, Assembly, and Proteolytic Processing of Helminthosporium victoriae 190S Totivirus Capsid Protein in Insect Cells

Expression, Assembly, and Proteolytic Processing of Helminthosporium victoriae 190S Totivirus Capsid Protein in Insect Cells

The Maze Pathway of Coevolution: A Critical Review over the Leishmania and Its Endosymbiotic History

Phylogenetic analysis ofLeishmaniaRNA virus andLeishmaniasuggests ancient virus-parasite association

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